Invitrogen

IL-6 Monoclonal Antibody (5IL6)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

5 Published Figures

24 References

View all (212) IL-6 antibodies

Datasheet

Protocols

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Datasheet

Protocols

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IL-6 Antibody (M620) in ELISA

Sandwich ELISA analysis of human IL-6 was performed using a Human IL-6 Colorimetric ELISA kit (Product # EH2IL6) by loading 50 µL per well of a mouse anti-human IL-6 biotinylated antibody (Product # M621B) at a concentration of 0.035 µg/mL and 50 µL of Human IL-6 Recombinant Protein (Product # SIL6) at 400, 160, 64, 25.6, 10.24, and 0 pg/mL (both in quadruplicate) across a 4 µg/mL mouse anti-human IL-6 (Product # M620) pre-coated plate. The plate was incubated for 2 hours at room temperature, washed, and incubated with 100 µL per well of S... View More

IL-6 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

IL-6 Antibody — Altered expression of proteins upon cell treatment demonstrates antibody specificity. Western blot using IL-6 Monoclonal Antibody (5IL6) (Product # M620), shows upregulation in expression of IL-6 upon treatement of HUVEC cells with LPS and Brefeldin A. {TM}

Product Details

M620

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

1:100

ELISA (ELISA)

Assay-dependent

View 16 publications 16 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Pig, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

5IL6

Immunogen

Recombinant human IL-6.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_223576

Product Specific Information

M620 was successfully to detect IL-6 in Flow application.

M620 targets IL-6 in ELISA, and Western blot applications and shows reactivity with human samples.

The M620 immunogen is recombinant human IL-6.

M620 detects IL-6 which has a predicted molecular weight of approximately 22 kDa.

The M620 IL6 antibody (clone 5IL6) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody M621B (biotinylated conjugate of clone 7IL6). Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection.

Antibody M620 (clone 5IL6) and biotinylated antibody M621B (clone 7IL6) have successfully been used in combination with recombinant IL6 protein SIL6 in ELISA applications.

This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).

Target Information

IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells. The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections. The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Neuroprotection of cannabidiol in epileptic rats: Gut microbiome and metabolome sequencing.

Frontiers in nutrition

Gong X,Liu L,Li X,Xiong J,Xu J,Mao D,Liu L

M620 was used in Western Blotting to investigate the effects of cannabidiol (CBD) on gut microbiota and the metabolic profile of epileptic rats.

Tue Dec 06 00:00:00 EST 2022

Hepcidin Increases Cytokines in Alzheimer's Disease and Down's Syndrome Dementia: Implication of Impaired Iron Homeostasis in Neuroinflammation.

Frontiers in aging neuroscience

Raha AA,Ghaffari SD,Henderson J,Chakraborty S,Allinson K,Friedland RP,Holland A,Zaman SH,Mukaetova-Ladinska EB,Raha-Chowdhury R

M620 was used in Immunohistochemistry to study levels of ferritin, hepcidin, and IL-16 in patients with Alzheimer's disease and Down's syndrome.

Tue May 18 00:00:00 EDT 2021

Hepcidin Increases Cytokines in Alzheimer's Disease and Down's Syndrome Dementia: Implication of Impaired Iron Homeostasis in Neuroinflammation.

Frontiers in aging neuroscience

Raha AA,Ghaffari SD,Henderson J,Chakraborty S,Allinson K,Friedland RP,Holland A,Zaman SH,Mukaetova-Ladinska EB,Raha-Chowdhury R

M620 was used in Immunohistochemistry to study levels of ferritin, hepcidin, and IL-16 in patients with Alzheimer's disease and Down's syndrome.

Tue May 18 00:00:00 EDT 2021

Impaired Iron Homeostasis and Haematopoiesis Impacts Inflammation in the Ageing Process in Down Syndrome Dementia.

Journal of clinical medicine

Raha-Chowdhury R,Raha AA,Henderson J,Ghaffari SD,Grigorova M,Beresford-Webb J,Allinson K,Chakraborty S,Holland A,Zaman SH

M620 was used in Immunohistochemistry, Western Blot to determine whether genetic variants and inflammatory proteins are involved in haematopoiesis and cellular processes in Down syndrome compared with age-matched control participants, particularly with respect to neuroinflammation and accelerated ageing.

Tue Jun 29 00:00:00 EDT 2021

Impaired Iron Homeostasis and Haematopoiesis Impacts Inflammation in the Ageing Process in Down Syndrome Dementia.

Journal of clinical medicine

Raha-Chowdhury R,Raha AA,Henderson J,Ghaffari SD,Grigorova M,Beresford-Webb J,Allinson K,Chakraborty S,Holland A,Zaman SH

Tue Jun 29 00:00:00 EDT 2021

Impaired Iron Homeostasis and Haematopoiesis Impacts Inflammation in the Ageing Process in Down Syndrome Dementia.

Journal of clinical medicine

Raha-Chowdhury R,Raha AA,Henderson J,Ghaffari SD,Grigorova M,Beresford-Webb J,Allinson K,Chakraborty S,Holland A,Zaman SH

Tue Jun 29 00:00:00 EDT 2021

Pulsed focused ultrasound enhances the therapeutic effect of mesenchymal stromal cell-derived extracellular vesicles in acute kidney injury.

Stem cell research & therapy

Ullah M,Liu DD,Rai S,Razavi M,Concepcion W,Thakor AS

Published figure using IL-6 monoclonal antibody (Product # M620) in Immunohistochemistry

Mon Sep 14 00:00:00 EDT 2020

Pulsed focused ultrasound enhances the therapeutic effect of mesenchymal stromal cell-derived extracellular vesicles in acute kidney injury.

Stem cell research & therapy

Ullah M,Liu DD,Rai S,Razavi M,Concepcion W,Thakor AS

Published figure using IL-6 monoclonal antibody (Product # M620) in Immunohistochemistry

Mon Sep 14 00:00:00 EDT 2020

Neutralization of interleukin-17A alleviates burn-induced intestinal barrier disruption via reducing pro-inflammatory cytokines in a mouse model.

Burns & trauma

Song Y,Li Y,Xiao Y,Hu W,Wang X,Wang P,Zhang X,Yang J,Huang Y,He W,Huang C

Published figure using IL-6 monoclonal antibody (Product # M620) in Western Blot

Tue Apr 12 00:00:00 EDT 2022

Sevoflurane exerts protective effects on liver ischemia/reperfusion injury by regulating NFKB3 expression via miR-9-5p.

Experimental and therapeutic medicine

Liao X,Zhou S,Zong J,Wang Z

M620 was used in an ELISA assay to study the protective effects of sevoflurane on hepatic ischemia/reperfusion liver injury.

Mon Apr 01 00:00:00 EDT 2019

Toll-Interleukin 1 Receptor Domain-Containing Adaptor Protein 180L Single-Nucleotide Polymorphism Is Associated With Susceptibility to Recurrent Pneumococcal Lower Respiratory Tract Infections in Children.

Frontiers in immunology

Siebert JN,Hamann L,Verolet CM,Gameiro C,Grillet S,Siegrist CA,Posfay-Barbe KM

M620 was used in an ELISA assay to asses whether a defect somewhere along this toll-like receptor signally pathways increases susceptibility to recurrent pneumococcal low respiratory tract infections.

Mon Sep 16 00:00:00 EDT 2019

Interleukin-6 is a potential therapeutic target in interleukin-6 dependent, estrogen receptor-α-positive breast cancer.

Breast cancer (Dove Medical Press)

Casneuf T,Axel AE,King P,Alvarez JD,Werbeck JL,Verhulst T,Verstraeten K,Hall BM,Sasser AK

M620 was used to discuss the role of IL-6 in estrogen receptor positive breast cancers

Fri Feb 19 00:00:00 EST 2016

Development and validation of panoptic Meso scale discovery assay to quantify total systemic interleukin-6.

British journal of clinical pharmacology

Chaturvedi S,Siegel D,Wagner CL,Park J,van de Velde H,Vermeulen J,Fung MC,Reddy M,Hall B,Sasser K

M620 was used in ELISA to develop a validated assay to measure complexed and non-complexed IL-6.

Thu Oct 01 00:00:00 EDT 2015

Development of an 8-plex Luminex assay to detect swine cytokines for vaccine development: assessment of immunity after porcine reproductive and respiratory syndrome virus (PRRSV) vaccination.

Vaccine

Lawson S,Lunney J,Zuckermann F,Osorio F,Nelson E,Welbon C,Clement T,Fang Y,Wong S,Kulas K,Christopher-Hennings J

M620 was used in ELISA to develop a multiplex Luminex assay to measure pig cytokines for vaccine development

Mon Jul 19 00:00:00 EDT 2010

Inhibitors of p38 suppress cytokine production in rheumatoid arthritis synovial membranes: does variable inhibition of interleukin-6 production limit effectiveness in vivo?

Arthritis and rheumatism

Page TH,Brown A,Timms EM,Foxwell BM,Ray KP

M620 was used in ELISA to investigate the role of p38 kinase in rheumatoid arthritis synovial membranes

Mon Nov 01 00:00:00 EDT 2010

A component of polysaccharide peptidoglycan complex on Lactobacillus induced an improvement of murine model of inflammatory bowel disease and colitis-associated cancer.

Immunology

Matsumoto S,Hara T,Nagaoka M,Mike A,Mitsuyama K,Sako T,Yamamoto M,Kado S,Takada T

M620 was used in ELISA to investigate the role of Lactobacillus polysaccharide peptidoglycan complex in chronic intestinal inflammatory disorders

Tue Sep 01 00:00:00 EDT 2009

Human mast cell activation with virus-associated stimuli leads to the selective chemotaxis of natural killer cells by a CXCL8-dependent mechanism.

Blood

Burke SM,Issekutz TB,Mohan K,Lee PW,Shmulevitz M,Marshall JS

M620 was used in ELISA to study the role of human mast cells in the recruitment of natural killer cells

Sun Jun 15 00:00:00 EDT 2008

IgE modulates neutrophil survival in asthma: role of mitochondrial pathway.

Journal of immunology (Baltimore, Md. : 1950)

Saffar AS,Alphonse MP,Shan L,Hayglass KT,Simons FE,Gounni AS

M620 was used in ELISA to study the role of mitochondrial pathway in neutrophil survival

Thu Feb 15 00:00:00 EST 2007

Protein microarray platform for the multiplex analysis of biomarkers in human sera.

Journal of immunological methods

Urbanowska T,Mangialaio S,Zickler C,Cheevapruk S,Hasler P,Regenass S,Legay F

M620 was used in ELISA to develop a protein microarray platform for the multiplex analysis of biomarkers in human sera

Fri Oct 20 00:00:00 EDT 2006

Probiotic Lactobacillus-induced improvement in murine chronic inflammatory bowel disease is associated with the down-regulation of pro-inflammatory cytokines in lamina propria mononuclear cells.

Clinical and experimental immunology

Matsumoto S,Hara T,Hori T,Mitsuyama K,Nagaoka M,Tomiyasu N,Suzuki A,Sata M

M620 was used in ELISA to study the effect of Lactobacillus casei strain Shirota on the production of interleukin 6 in LPS-stimulated murine large intestinal lamina propria mononuclear cells

Wed Jun 01 00:00:00 EDT 2005

Long-term treatment with etanercept significantly reduces the number of proinflammatory cytokine-secreting peripheral blood mononuclear cells in patients with rheumatoid arthritis.

Rheumatology (Oxford, England)

Schotte H,Schlüter B,Willeke P,Mickholz E,Schorat MA,Domschke W,Gaubitz M

M620 was used in ELISA to study the effect of etanercept treatment on proinflammatory cytokine-secreting peripheral blood mononuclear cells in rheumatoid arthritic patients

Sun Aug 01 00:00:00 EDT 2004

Serum cytokine levels, cigarette smoking and airway responsiveness among pregnant women.

International archives of allergy and immunology

Tsunoda M,Litonjua AA,Kuniak MP,Weiss ST,Satoh T,Guevarra L,Tollerud DJ

M620 was used in ELISA to investigate the relationship between serum cytokine levels, cigarette smoking and airway responsiveness among pregnant women

Sat Feb 01 00:00:00 EST 2003

A nonsteroidal glucocorticoid receptor antagonist.

Molecular endocrinology (Baltimore, Md.)

Miner JN,Tyree C,Hu J,Berger E,Marschke K,Nakane M,Coghlan MJ,Clemm D,Lane B,Rosen J

M620 was used in immunocytochemistry to characterize a potent nonsteroidal antagonist against glucocorticoid receptor

Wed Jan 01 00:00:00 EST 2003

Ambient air particulates stimulate alveolar macrophages of smokers to promote differentiation of myeloid precursor cells.

Experimental lung research

Suwa T,Hogg JC,Vincent R,Mukae H,Fujii T,van Eeden SF

M620 was used in ELISA to test if factors released from human alveolar macrophages exposed to air pollution particles accelerate the maturation of granulocyte precursors

Tue Jul 09 00:00:00 EDT 2002

Release of vasoactive cytokines by antibody-enhanced dengue virus infection of a human mast cell/basophil line.

Journal of virology

King CA,Marshall JS,Alshurafa H,Anderson R

M620 was used in ELISA to investigate the role of mast cells/basophils in dengue pathogenesis

Tue Aug 01 00:00:00 EDT 2000

Regulation of interleukin-12 by complement receptor 3 signaling.

The Journal of experimental medicine

Marth T,Kelsall BL

M620 was used in ELISA to assess the interferon-alpha production in peripheral blood mononuclear cell

Mon Jun 02 00:00:00 EDT 1997

Sustained high levels of circulating chaperoned interleukin-6 after active specific cancer immunotherapy.

Blood

May LT,Patel K,García D,Ndubuisi MI,Ferrone S,Mittelman A,Mackiewicz A,Sehgal PB

M620 was used in ELISA to investigate the changes of interleukin 6 level in patients with cancer immunotherapy

Thu Sep 15 00:00:00 EDT 1994

Bioinformatics

Protein Aliases: B-cell differentiation factor; B-cell hybridoma growth factor; B-cell stimulatory factor 2; BSF-2; CDF; CTL differentiation factor; H-IL-6; Hybridoma growth factor; IFN-beta-2; IL-6; ILN; Interferon beta-2; Interleukin; interleukin BSF-2; Interleukin HP-1; Interleukin-6; Interleukin6; M-IL-6; R-IL-6

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Gene Aliases: BSF-2; BSF2; CDF; HGF; HSF; IFN-beta-2; IFNB2; IL-6; IL6

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UniProt ID: (Human) P05231, (Mouse) P08505

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Entrez Gene ID: (Human) 3569, (Mouse) 16193

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Function(s)

cytokine activity interleukin-6 receptor binding protein binding growth factor activity receptor binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IL-6 Monoclonal Antibody (5IL6)

IL-6 Antibody (M620) in ELISA

Product Details

M620

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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