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Invitrogen
IL-6 Monoclonal Antibody (6708)
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 3
IL-6 Antibody (MA5-23698) in WB
Western blot was performed using Anti-IL-6 Monoclonal Antibody (6708) (Product # MA5-23698) and a 24 kDa band corresponding to IL-6 was observed across all cell treatments tested. Whole cell extracts (50 µg lysate) of HUVEC (Lane 1), HUVEC treated with LPS and Brefeldin A (0.5 µg/mL of LPS for 24 h and 300 ng/mL of Brefeldin A for last 20 h of LPS stimulation) (Lane 2), HaCaT (Lane 3), HaCaT transfected with Poly(I:C) and treated with PTI (0.67 µg/mL and 1x respectively, for 4 h) (Lane 4), HaCaT (Lane 5) and HaCaT transfected with Poly(dA:... View More
Product Details
MA5-23698
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate
Published species
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
6708
Immunogen
E. coli-derived recombinant human IL-6
Conjugate
Form
Lyophilized
Concentration
0.5 mg/mL
Purification
Protein A/G
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2609708
Product Specific Information
In Western blots, this antibody does not cross-react with recombinant mouse (rm) IL-6, rhOSM, rhLIF, rhIL-11, rhgp130, or rhCNTF.
Reconstitute at 0.5 mg/mL in sterile PBS.
Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.
Target Information
IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells. The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections. The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: B-cell differentiation factor; B-cell hybridoma growth factor; B-cell stimulatory factor 2; BSF-2; CDF; CTL differentiation factor; H-IL-6; Hybridoma growth factor; IFN-beta-2; IL-6; ILN; Interferon beta-2; Interleukin; interleukin BSF-2; Interleukin HP-1; Interleukin-6; Interleukin6; M-IL-6; R-IL-6
Gene Aliases: BSF-2; BSF2; CDF; HGF; HSF; IFN-beta-2; IFNB2; IL-6; IL6
UniProt ID: (Human) P05231, (Mouse) P08505
Entrez Gene ID: (Human) 3569, (Mouse) 16193
neutrophil apoptotic process
myeloid cell homeostasis
hepatic immune response
positive regulation of acute inflammatory response
negative regulation of protein kinase activity
acute-phase response
inflammatory response
immune response
positive regulation of cell proliferation
negative regulation of cell proliferation
regulation of cell shape
response to wounding
regulation of vascular endothelial growth factor production
positive regulation of gene expression
cell growth
cytokine-mediated signaling pathway
endocrine pancreas development
neuron projection development
positive regulation of chemokine production
positive regulation of interleukin-6 production
negative regulation of collagen biosynthetic process
positive regulation of peptidyl-serine phosphorylation
positive regulation of protein import into nucleus, translocation
positive regulation of T cell proliferation
T cell activation
regulation of cell proliferation
positive regulation of tyrosine phosphorylation of Stat3 protein
glucose homeostasis
defense response to protozoan
regulation of apoptotic process
positive regulation of apoptotic process
negative regulation of apoptotic process
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
positive regulation of MAPK cascade
negative regulation of chemokine biosynthetic process
regulation of circadian sleep/wake cycle, non-REM sleep
positive regulation of nitric oxide biosynthetic process
cell redox homeostasis
positive regulation of T-helper 2 cell differentiation
positive regulation of neuron differentiation
negative regulation of gluconeogenesis
positive regulation of translation
positive regulation of DNA replication
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of JAK-STAT cascade
muscle cell cellular homeostasis
negative regulation of hormone secretion
negative regulation of muscle organ development
positive regulation of smooth muscle cell proliferation
positive regulation of epithelial cell proliferation
negative regulation of cytokine secretion
positive regulation of peptidyl-tyrosine phosphorylation
positive regulation of B cell activation
positive regulation of immunoglobulin secretion
positive regulation of sequence-specific DNA binding transcription factor activity
response to glucocorticoid
defense response to virus
positive regulation of protein kinase B signaling
positive regulation of transmission of nerve impulse
branching involved in salivary gland morphogenesis
epithelial cell proliferation involved in salivary gland morphogenesis
glucagon secretion
interleukin-6-mediated signaling pathway
cellular response to hydrogen peroxide
positive regulation of ERK1 and ERK2 cascade
cellular response to lipopolysaccharide
cellular response to interleukin-1
cellular response to tumor necrosis factor
T-helper 17 cell lineage commitment
negative regulation of neuron death
response to yeast
neutrophil mediated immunity
monocyte chemotaxis
positive regulation of leukocyte chemotaxis
humoral immune response
aging
response to heat
response to cold
negative regulation of lipid storage
response to auditory stimulus
platelet activation
response to caffeine
response to nutrient levels
response to peptidoglycan
response to insulin
cellular response to hepatocyte growth factor stimulus
response to drug
response to amino acid
negative regulation of fat cell differentiation
positive regulation of osteoblast differentiation
regulation of angiogenesis
response to antibiotic
bone remodeling
defense response to Gram-negative bacterium
defense response to Gram-positive bacterium
positive regulation of NF-kappaB transcription factor activity
response to calcium ion
response to electrical stimulus
cellular response to dexamethasone stimulus
positive regulation of cell proliferation in bone marrow
positive regulation of STAT protein import into nucleus
positive regulation of type B pancreatic cell apoptotic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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