Invitrogen

IL-6 Monoclonal Antibody (MP5-20F3), FITC, eBioscience™

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

24 Published Figures

29 References

View all (212) IL-6 antibodies

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Protocols

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IL-6 Antibody (11-7061-82) in Flow

Mouse thiogylcolate-induced peritoneal exudate cells were stimulated with LPS in the presence of Brefeldin A (Product # 00-4506-51) overnight. The cells were surface stained with Anti-Mouse F4/80 Antigen APC (Product # 17-4801-82) and intracellularly stained with 0.25 µg of Rat IgG1 K Isotype Control FITC (left) or 0.25 µg of Anti-Mouse IL-6 FITC (right). Total events were used for analysis.

IL-6 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

IL-6 Antibody in Immunocytochemistry (ICC/IF)

IL-6 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

IL-6 Antibody in Immunohistochemistry (IHC)

IL-6 Antibody in Immunohistochemistry, ELISA (IHC, ELISA)

Product Image Gallery

IL-6 Antibody — Figure 6 Local s.c. treatment with 1,25(OH) 2 D during the proinflammatory stage suppressed at fracture sites CD11b + F4/80 + macrophage secretion of M1 macrophage-associated molecules that had been shown to promote osteogenesis in MSCs and to be important for fracture repair. B6 mice were subjected to fracture surgery (Fx), treatments, cell isolation, and analyses as described in Figure 3 . The cells from days 1, 4, and 7 were analyzed. Data from day 1 are shown. ( A ) Representative FACS histograms show the expressions of Oncostatin M (OSM, upper panel), TNF-alpha (middle panel), and IL-6 (lower panel) in CD11b + F4/80 + macrophages. ( B ) Left, cumulative data of MFIs of OSM, TNF-alpha, and IL-6 expressions in CD11b + F4/80 + macrophages. Right, percent of OSM hi , TNF-alpha hi , and IL-6 hi cells among CD11b + F4/80 + macrophages. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, ANOVA, n = 3. {TM}

Product Details

11-7061-82

Applications

Tested Dilution

Publications

Western Blot (WB)

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

View 3 publications 3 publications

Flow Cytometry (Flow)

0.5 µg/test

View 12 publications 12 publications

ELISA (ELISA)

View 6 publications 6 publications

Neutralization (Neu)

View 4 publications 4 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG1, kappa

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MP5-20F3

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_465394

Product Specific Information

Description: The MP5-20F3 antibody reacts with mouse interleukin-6 (IL-6). The MP5-20F3 antibody is a neutralizing antibody. Mouse IL-6 (also known as B Cell Stimulating Factor, BCSF, and BSF-2) is a 21-28 kDa factor produced by a variety of cell types, including monocytes, fibroblasts and endothelial cells. Upon stimulation, macrophages, T, B, mast, and glial cells, eosinophils, keratinocytes and granulocytes also secrete IL-6. This pleiotrophic cytokine influences the antigen-specific immune response, inflammatory response, and the development of neurons, and is a major mediator of the acute phase reaction.

Applications Reported:This MP5-20F3 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: Has been tested by intracellular staining with flow cytometric analysis. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells. The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections. The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Zika Virus Infection Induces Interleukin-1β-Mediated Inflammatory Responses by Macrophages in the Brain of an Adult Mouse Model.

Journal of virology

Jeong GU,Lee S,Kim DY,Lyu J,Yoon GY,Kim KD,Ku KB,Ko J,Kwon YC

Published figure using IL-6 monoclonal antibody (Product # 11-7061-82) in Flow Cytometry

Thu Jun 29 00:00:00 EDT 2023

A low-carbohydrate diet induces hepatic insulin resistance and metabolic associated fatty liver disease in mice.

Molecular metabolism

Long F,Bhatti MR,Kellenberger A,Sun W,Modica S,Höring M,Liebisch G,Krieger JP,Wolfrum C,Challa TD

Published figure using IL-6 monoclonal antibody (Product # 11-7061-82) in Neutralization

Wed Mar 01 00:00:00 EST 2023

Lin28B-high breast cancer cells promote immune suppression in the lung pre-metastatic niche via exosomes and support cancer progression.

Nature communications

Qi M,Xia Y,Wu Y,Zhang Z,Wang X,Lu L,Dai C,Song Y,Xu K,Ji W,Zhan L

Published figure using IL-6 monoclonal antibody (Product # 11-7061-82) in Flow Cytometry

Wed Feb 16 00:00:00 EST 2022

Sox2 overexpression alleviates noise-induced hearing loss by inhibiting inflammation-related hair cell apoptosis.

Journal of neuroinflammation

Chen D,Jia G,Zhang Y,Mao H,Zhao L,Li W,Chen Y,Ni Y

11-7061-82 was used in Immunohistochemistry, Western Blot to demonstrate a novel protective role of Sox2 in mature and terminally differentiated cochlear hair cells by inhibiting inflammation.

Mon Feb 28 00:00:00 EST 2022

Sox2 overexpression alleviates noise-induced hearing loss by inhibiting inflammation-related hair cell apoptosis.

Journal of neuroinflammation

Chen D,Jia G,Zhang Y,Mao H,Zhao L,Li W,Chen Y,Ni Y

11-7061-82 was used in Immunohistochemistry, Western Blot to demonstrate a novel protective role of Sox2 in mature and terminally differentiated cochlear hair cells by inhibiting inflammation.

Mon Feb 28 00:00:00 EST 2022

Sox2 overexpression alleviates noise-induced hearing loss by inhibiting inflammation-related hair cell apoptosis.

Journal of neuroinflammation

Chen D,Jia G,Zhang Y,Mao H,Zhao L,Li W,Chen Y,Ni Y

11-7061-82 was used in Immunohistochemistry, Western Blot to demonstrate a novel protective role of Sox2 in mature and terminally differentiated cochlear hair cells by inhibiting inflammation.

Mon Feb 28 00:00:00 EST 2022

Sox2 overexpression alleviates noise-induced hearing loss by inhibiting inflammation-related hair cell apoptosis.

Journal of neuroinflammation

Chen D,Jia G,Zhang Y,Mao H,Zhao L,Li W,Chen Y,Ni Y

11-7061-82 was used in Immunohistochemistry, Western Blot to demonstrate a novel protective role of Sox2 in mature and terminally differentiated cochlear hair cells by inhibiting inflammation.

Mon Feb 28 00:00:00 EST 2022

SARS-CoV-2 Infection of Microglia Elicits Proinflammatory Activation and Apoptotic Cell Death.

Microbiology spectrum

Jeong GU,Lyu J,Kim KD,Chung YC,Yoon GY,Lee S,Hwang I,Shin WH,Ko J,Lee JY,Kwon YC

11-7061-82 was used in Flow cytometry/Cell sorting to report that SARS-CoV-2 can directly infect human microglia, eliciting M1-like proinflammatory responses, followed by cytopathic effects.

Wed Jun 29 00:00:00 EDT 2022

Tumor-derived IL-6 and trans-signaling among tumor, fat, and muscle mediate pancreatic cancer cachexia.

The Journal of experimental medicine

Rupert JE,Narasimhan A,Jengelley DHA,Jiang Y,Liu J,Au E,Silverman LM,Sandusky G,Bonetto A,Cao S,Lu X,O'Connell TM,Liu Y,Koniaris LG,Zimmers TA

Published figure using IL-6 monoclonal antibody (Product # 11-7061-82) in Neutralization

Mon Jun 07 00:00:00 EDT 2021

Restoration of 5-methoxytryptophan protects against atherosclerotic chondrogenesis and calcification in ApoE^-/- mice fed high fat diet.

Journal of biomedical science

Lee GL,Liao TL,Wu JY,Wu KK,Kuo CC

Published figure using IL-6 monoclonal antibody (Product # 11-7061-82) in ELISA

Mon Nov 08 00:00:00 EST 2021

TNF-Like Ligand 1 Aberrance Aggravates Nonalcoholic Steatohepatitis via M1 Macrophage Polarization.

Oxidative medicine and cellular longevity

Luo Y,Guo J,Jia W,Wu M,Yin F,Niu G,Shih DQ,Targan SR,Zhang X

Published figure using IL-6 monoclonal antibody (Product # 11-7061-82) in Flow Cytometry

Fri Mar 04 00:00:00 EST 2022

Murine Model of Sinusitis Infection for Screening Antimicrobial and Immunomodulatory Therapies.

Frontiers in cellular and infection microbiology

Alford MA,Choi KG,Trimble MJ,Masoudi H,Kalsi P,Pletzer D,Hancock REW

Published figure using IL-6 monoclonal antibody (Product # 11-7061-82) in ELISA

Fri Jul 02 00:00:00 EDT 2021

Betulinic Acid Ameliorates the Severity of Acute Pancreatitis via Inhibition of the NF-κB Signaling Pathway in Mice.

International journal of molecular sciences

Zhou Z,Choi JW,Shin JY,Kim DU,Kweon B,Oh H,Kim YC,Song HJ,Bae GS,Park SJ

11-7061-82 was used in ELISA and Immunocytochemistry to suggest that botulinic acid may have prophylactic and therapeutic effects on AP via inhibition of the NF-κB signaling pathway.

Sat Jun 26 00:00:00 EDT 2021

Betulinic Acid Ameliorates the Severity of Acute Pancreatitis via Inhibition of the NF-κB Signaling Pathway in Mice.

International journal of molecular sciences

Zhou Z,Choi JW,Shin JY,Kim DU,Kweon B,Oh H,Kim YC,Song HJ,Bae GS,Park SJ

11-7061-82 was used in ELISA and Immunocytochemistry to suggest that botulinic acid may have prophylactic and therapeutic effects on AP via inhibition of the NF-κB signaling pathway.

Sat Jun 26 00:00:00 EDT 2021

Elevated type I interferon responses potentiate metabolic dysfunction, inflammation, and accelerated aging in mtDNA mutator mice.

Science advances

Lei Y,Guerra Martinez C,Torres-Odio S,Bell SL,Birdwell CE,Bryant JD,Tong CW,Watson RO,West LC,West AP

11-7061 was used in Flow cytometry/Cell sorting to further advance our knowledge of how mitochondrial dysfunction shapes innate immune responses and provide a framework for understanding mitochondria-driven immunopathology in POLG-related disorders and aging.

Sat May 01 00:00:00 EDT 2021

Spatial proteomics revealed a CX₃CL1-dependent crosstalk between the urothelium and relocated macrophages through IL-6 during an acute bacterial infection in the urinary bladder.

Mucosal immunology

Bottek J,Soun C,Lill JK,Dixit A,Thiebes S,Beerlage AL,Horstmann M,Urbanek A,Heuer H,Uszkoreit J,Eisenacher M,Bracht T,Sitek B,Hoffmann F,Vijitha N,von Eggeling F,Engel DR

Published figure using IL-6 monoclonal antibody (Product # 11-7061-82) in ELISA

Wed Jul 01 00:00:00 EDT 2020

Spatial proteomics revealed a CX₃CL1-dependent crosstalk between the urothelium and relocated macrophages through IL-6 during an acute bacterial infection in the urinary bladder.

Mucosal immunology

Bottek J,Soun C,Lill JK,Dixit A,Thiebes S,Beerlage AL,Horstmann M,Urbanek A,Heuer H,Uszkoreit J,Eisenacher M,Bracht T,Sitek B,Hoffmann F,Vijitha N,von Eggeling F,Engel DR

Published figure using IL-6 monoclonal antibody (Product # 11-7061-82) in ELISA

Wed Jul 01 00:00:00 EDT 2020

Regulating colonic dendritic cells by commensal glycosylated large surface layer protein A to sustain gut homeostasis against pathogenic inflammation.

Mucosal immunology

Ge Y,Gong M,Zadeh M,Li J,Abbott JR,Li W,Morel L,Sonon R,Supekar NT,Azadi P,Wang Y,Jones DP,Li S,Mohamadzadeh M

11-7061 was used in Flow cytometry/Cell sorting to study the molecular mechanisms responsible for regulating gut homeostasis.

Wed Jan 01 00:00:00 EST 2020

Senolytics prevent mt-DNA-induced inflammation and promote the survival of aged organs following transplantation.

Nature communications

Iske J,Seyda M,Heinbokel T,Maenosono R,Minami K,Nian Y,Quante M,Falk CS,Azuma H,Martin F,Passos JF,Niemann CU,Tchkonia T,Kirkland JL,Elkhal A,Tullius SG

11-7061 was used in Flow cytometry/Cell sorting to show that cell-free mitochondrial DNA (cf-mt-DNA) released by senescent cells accumulates with aging and augments immunogenicity.

Thu Aug 27 00:00:00 EDT 2020

Peli1 controls the survival of dopaminergic neurons through modulating microglia-mediated neuroinflammation.

Scientific reports

Dai D,Yuan J,Wang Y,Xu J,Mao C,Xiao Y

Published figure using IL-6 monoclonal antibody (Product # 11-7061-82) in ELISA

Wed May 29 00:00:00 EDT 2019

Vanadium Derivative Exposure Promotes Functional Alterations of VSMCs and Consequent Atherosclerosis via ROS/p38/NF-κB-Mediated IL-6 Production.

International journal of molecular sciences

Yeh CC,Wu JY,Lee GL,Wen HT,Lin P,Kuo CC

Published figure using IL-6 monoclonal antibody (Product # 11-7061-82) in ELISA

Wed Dec 04 00:00:00 EST 2019

1,25-Dihydroxyvitamin D suppresses M1 macrophages and promotes M2 differentiation at bone injury sites.

JCI insight

Wasnik S,Rundle CH,Baylink DJ,Yazdi MS,Carreon EE,Xu Y,Qin X,Lau KW,Tang X

11-7061 was used in Flow cytometry/Cell sorting to define the role of M1 macrophages at bone injury sites via the function of 1,25-Dihydroxyvitamin D in suppressing M1 but promoting M2 differentiation.

Thu Sep 06 00:00:00 EDT 2018

Hepatic inflammation caused by dysregulated bile acid synthesis is reversible by butyrate supplementation.

The Journal of pathology

Sheng L,Jena PK,Hu Y,Liu HX,Nagar N,Kalanetra KM,French SW,French SW,Mills DA,Wan YY

Published figure using IL-6 monoclonal antibody (Product # 11-7061-82) in Western Blot

Fri Dec 01 00:00:00 EST 2017

Depletion of microglia exacerbates postischemic inflammation and brain injury.

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism

Jin WN,Shi SX,Li Z,Li M,Wood K,Gonzales RJ,Liu Q

Published figure using IL-6 monoclonal antibody (Product # 11-7061-82) in Flow Cytometry

Thu Jun 01 00:00:00 EDT 2017

Tumor-associated macrophages promote neuroblastoma via STAT3 phosphorylation and up-regulation of c-MYC.

Oncotarget

Hadjidaniel MD,Muthugounder S,Hung LT,Sheard MA,Shirinbak S,Chan RY,Nakata R,Borriello L,Malvar J,Kennedy RJ,Iwakura H,Akamizu T,Sposto R,Shimada H,DeClerck YA,Asgharzadeh S

11-7061 was used in Flow cytometry/Cell sorting to indicate a novel role of tumour-associated macrophages as inducers of MYC expression in neuroblastomas lacking independent oncogene activation.

Tue Oct 31 00:00:00 EDT 2017

Sprouty1 induces a senescence-associated secretory phenotype by regulating NFκB activity: implications for tumorigenesis.

Cell death and differentiation

Macià A,Vaquero M,Gou-Fàbregas M,Castelblanco E,Valdivielso JM,Anerillas C,Mauricio D,Matias-Guiu X,Ribera J,Encinas M

Published figure using IL-6 monoclonal antibody (Product # 11-7061-82) in Neutralization

Sat Feb 01 00:00:00 EST 2014

Live single cell functional phenotyping in droplet nano-liter reactors.

Scientific reports

Konry T,Golberg A,Yarmush M

Published figure using IL-6 monoclonal antibody (Product # 11-7061-82) in Immunofluorescence

Mon Nov 11 00:00:00 EST 2013

Osteoclast activated FoxP3+ CD8+ T-cells suppress bone resorption in vitro.

PloS one

Buchwald ZS,Kiesel JR,DiPaolo R,Pagadala MS,Aurora R

11-7061 was used in Flow cytometry/Cell sorting to demonstrate the effects of activated CD8 T cell-produced cytokines on osteoclast activity.

Thu Nov 08 00:00:00 EST 2012

CD8 regulates T regulatory cell production of IL-6 and maintains their suppressive phenotype in allergic lung disease.

Journal of immunology (Baltimore, Md. : 1950)

Joetham A,Okamoto M,Takeda K,Schedel M,Ohnishi H,Dakhama A,Gelfand EW

11-7061 was used in Flow cytometry/Cell sorting to identify the critical role of CD8-T regulatory cell interactions in regulating the suppressive phenotype of nTregs through control of IL-6 production.

Sat Jan 01 00:00:00 EST 2011

IL-6 produced by immune complex-activated follicular dendritic cells promotes germinal center reactions, IgG responses and somatic hypermutation.

International immunology

Wu Y,El Shikh ME,El Sayed RM,Best AM,Szakal AK,Tew JG

Published figure using IL-6 monoclonal antibody (Product # 11-7061-82) in Neutralization

Mon Jun 01 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: B-cell hybridoma growth factor; H-IL-6; IL-6; ILN; Interleukin; Interleukin HP-1; Interleukin-6; Interleukin6; M-IL-6; R-IL-6

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Gene Aliases: Il-6; Il6

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UniProt ID: (Mouse) P08505

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Entrez Gene ID: (Mouse) 16193

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Function(s)

receptor binding cytokine activity interleukin-6 receptor binding protein binding growth factor activity

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IL-6 Monoclonal Antibody (MP5-20F3), FITC, eBioscience™

IL-6 Antibody (11-7061-82) in Flow

Product Details

11-7061-82

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Neutralization (Neu)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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