Invitrogen

IL-6 Monoclonal Antibody (MP5-20F3), PE, eBioscience™

FIGURE: 1 / 8

IL-6 Antibody (12-7061-82) in Flow

Mouse thioglycolate-induced peritoneal exudate cells were stimulated with LPS in the presence of Brefeldin A for 6 hours (right) or unstimulated (left). The cells were surface stained with Anti-Mouse F4/80 Antigen FITC (Product # 11-4801-82) and intracellularly stained with Anti-Mouse IL-6 PE.

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Product Details

12-7061-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.25 µg/test

View 17 publications 17 publications

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG1, kappa

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MP5-20F3

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_466165

Product Specific Information

Description: The MP5-20F3 antibody reacts with mouse interleukin-6 (IL-6). The MP5-20F3 antibody is a neutralizing antibody. Mouse IL-6 (also known as B Cell Stimulating Factor, BCSF, and BSF-2) is a 21-28 kDa factor produced by a variety of cell types, including monocytes, fibroblasts and endothelial cells. Upon stimulation, macrophages, T, B, mast, and glial cells, eosinophils, keratinocytes and granulocytes also secrete IL-6. This pleiotrophic cytokine influences the antigen-specific immune response, inflammatory response, and the development of neurons, and is a major mediator of the acute phase reaction.

Applications Reported:This MP5-20F3 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: Has been tested by intracellular staining and flow cytometric analysis of stimulated peritoneal macrophages. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.- test size: has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated peritoneal macrophages. This can be used at test size: 5 µL (0.125 µg) per test.The pre-titrated test size contains BSA and/or gelatin for protein stabilization.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells. The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections. The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Zika Virus Infection Induces Interleukin-1β-Mediated Inflammatory Responses by Macrophages in the Brain of an Adult Mouse Model.

Journal of virology

Jeong GU,Lee S,Kim DY,Lyu J,Yoon GY,Kim KD,Ku KB,Ko J,Kwon YC

Published figure using IL-6 monoclonal antibody (Product # 12-7061-82) in Flow Cytometry

Thu Jun 29 00:00:00 EDT 2023

Lin28B-high breast cancer cells promote immune suppression in the lung pre-metastatic niche via exosomes and support cancer progression.

Nature communications

Qi M,Xia Y,Wu Y,Zhang Z,Wang X,Lu L,Dai C,Song Y,Xu K,Ji W,Zhan L

Published figure using IL-6 monoclonal antibody (Product # 12-7061-82) in Flow Cytometry

Wed Feb 16 00:00:00 EST 2022

TNF-Like Ligand 1 Aberrance Aggravates Nonalcoholic Steatohepatitis via M1 Macrophage Polarization.

Oxidative medicine and cellular longevity

Luo Y,Guo J,Jia W,Wu M,Yin F,Niu G,Shih DQ,Targan SR,Zhang X

12-7061-82 was used in Flow cytometry/Cell sorting to investigate the role of TNF-like ligand 1 aberrance (TL1A) in nonalcoholic steatohepatitis (NASH).

Fri Mar 04 00:00:00 EST 2022

TNF-Like Ligand 1 Aberrance Aggravates Nonalcoholic Steatohepatitis via M1 Macrophage Polarization.

Oxidative medicine and cellular longevity

Luo Y,Guo J,Jia W,Wu M,Yin F,Niu G,Shih DQ,Targan SR,Zhang X

12-7061-82 was used in Flow cytometry/Cell sorting to investigate the role of TNF-like ligand 1 aberrance (TL1A) in nonalcoholic steatohepatitis (NASH).

Fri Mar 04 00:00:00 EST 2022

Osteopontin Expression Identifies a Subset of Recruited Macrophages Distinct from Kupffer Cells in the Fatty Liver.

Immunity

Remmerie A,Martens L,Thoné T,Castoldi A,Seurinck R,Pavie B,Roels J,Vanneste B,De Prijck S,Vanhockerhout M,Binte Abdul Latib M,Devisscher L,Hoorens A,Bonnardel J,Vandamme N,Kremer A,Borghgraef P,Van Vlierberghe H,Lippens S,Pearce E,Saeys Y,Scott CL

12-7061 was used in Flow cytometry/Cell sorting to highlight considerable heterogeneity within the macrophage pool and suggest a need for more specific macrophage targeting strategies in metabolic-associated fatty liver disease (MAFLD).

Tue Sep 15 00:00:00 EDT 2020

Protective role of follicular CXCR5⁺CD8⁺ T cells against dengue virus 2 infection.

International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases

Qiu L,Wang H,Yu Q,Liu J,Chen S,Zhao Z

12-7061-82 was used in Flow Cytometry to show that CXCR5+CD8+ T cells could play a protective role in dengue pathogenesis and may be a novel strategy for controlling DENV2 infection and vaccine development.

Sat Jun 01 00:00:00 EDT 2019

STAT6/Arg1 promotes microglia/macrophage efferocytosis and inflammation resolution in stroke mice.

JCI insight

Cai W,Dai X,Chen J,Zhao J,Xu M,Zhang L,Yang B,Zhang W,Rocha M,Nakao T,Kofler J,Shi Y,Stetler RA,Hu X,Chen J

12-7061 was used in Flow cytometry/Cell sorting to suggest that efferocytosis via STAT6/Arg1 modulates microglia/macrophage phenotype, accelerates inflammation resolution, and improves stroke outcomes.

Thu Oct 17 00:00:00 EDT 2019

Loss of NF-κB1 Causes Gastric Cancer with Aberrant Inflammation and Expression of Immune Checkpoint Regulators in a STAT-1-Dependent Manner.

Immunity

O'Reilly LA,Putoczki TL,Mielke LA,Low JT,Lin A,Preaudet A,Herold MJ,Yaprianto K,Tai L,Kueh A,Pacini G,Ferrero RL,Gugasyan R,Hu Y,Christie M,Wilcox S,Grumont R,Griffin MDW,O'Connor L,Smyth GK,Ernst M,Waring P,Gerondakis S,Strasser A

12-7061-82 was used in Flow Cytometry to provide mechanistic insight into how polymorphisms that attenuate NFKB1 expression predispose humans to epithelial cancers, highlighting the pro-tumorigenic activity of STAT1 and identifying targetable vulnerabilities in gastric cancer.

Tue Mar 20 00:00:00 EDT 2018

1,25-Dihydroxyvitamin D suppresses M1 macrophages and promotes M2 differentiation at bone injury sites.

JCI insight

Wasnik S,Rundle CH,Baylink DJ,Yazdi MS,Carreon EE,Xu Y,Qin X,Lau KW,Tang X

Published figure using IL-6 monoclonal antibody (Product # 12-7061-82) in Flow Cytometry

Thu Sep 06 00:00:00 EDT 2018

Depletion of microglia exacerbates postischemic inflammation and brain injury.

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism

Jin WN,Shi SX,Li Z,Li M,Wood K,Gonzales RJ,Liu Q

Thu Jun 01 00:00:00 EDT 2017

Depletion of microglia exacerbates postischemic inflammation and brain injury.

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism

Jin WN,Shi SX,Li Z,Li M,Wood K,Gonzales RJ,Liu Q

Thu Jun 01 00:00:00 EDT 2017

IL-6 Regulates M2 Polarization and Local Proliferation of Adipose Tissue Macrophages in Obesity.

Journal of immunology (Baltimore, Md. : 1950)

Braune J,Weyer U,Hobusch C,Mauer J,Brüning JC,Bechmann I,Gericke M

12-7061 was used in Flow cytometry/Cell sorting to study how IL-6 acts as a Th2 cytokine in obesity by stimulating M2 polarisation and local adipose tissue macrophages proliferation.

Sat Apr 01 00:00:00 EDT 2017

Identification of 14-dehydroergosterol as a novel anti-inflammatory compound inducing tolerogenic dendritic cells.

Scientific reports

Ano Y,Ikado K,Shindo K,Koizumi H,Fujiwara D

12-7061 was used in Flow cytometry/Cell sorting to investigate if 14-dehydroergosterol induces tolerogenic dendritic cells (DCs) to convert to a tolerogenic type.

Tue Oct 24 00:00:00 EDT 2017

Intestinal barrier dysfunction develops at the onset of experimental autoimmune encephalomyelitis, and can be induced by adoptive transfer of auto-reactive T cells.

PloS one

Nouri M,Bredberg A,Weström B,Lavasani S

12-7061 was used in Flow cytometry/Cell sorting to show that disruption of intestinal homeostasis is an early and immune-mediated event in experimental autoimmune encephalomyelitis.

Thu May 14 00:00:00 EDT 2015

Tumor suppressor p53 inhibits systemic autoimmune diseases by inducing regulatory T cells.

Journal of immunology (Baltimore, Md. : 1950)

Kawashima H,Takatori H,Suzuki K,Iwata A,Yokota M,Suto A,Minamino T,Hirose K,Nakajima H

12-7061 was used in Flow cytometry/Cell sorting to investigate how p53 suppresses autoimmunity.

Tue Oct 01 00:00:00 EDT 2013

Parvovirus evades interferon-dependent viral control in primary mouse embryonic fibroblasts.

Virology

Mattei LM,Cotmore SF,Tattersall P,Iwasaki A

12-7061 was used in Flow cytometry/Cell sorting to suggest that minute virus of mice efficiently evades antiviral immune mechanisms of type I IFNs, explaining their efficient transmission between mice.

Sat Jul 20 00:00:00 EDT 2013

CD8 regulates T regulatory cell production of IL-6 and maintains their suppressive phenotype in allergic lung disease.

Journal of immunology (Baltimore, Md. : 1950)

Joetham A,Okamoto M,Takeda K,Schedel M,Ohnishi H,Dakhama A,Gelfand EW

12-7061 was used in Flow cytometry/Cell sorting to identify the critical role of CD8-T regulatory cell interactions in regulating the suppressive phenotype of nTregs through control of IL-6 production.

Sat Jan 01 00:00:00 EST 2011

Mechanisms by which chronic ethanol feeding limits the ability of dendritic cells to stimulate T-cell proliferation.

Alcoholism, clinical and experimental research

Fan J,Edsen-Moore MR,Turner LE,Cook RT,Legge KL,Waldschmidt TJ,Schlueter AJ

12-7061 was used in Flow cytometry/Cell sorting to assess the functional capability of ethanol-exposed dendritic cells, using a murine model of chronic alcohol abuse.

Sat Jan 01 00:00:00 EST 2011

Mast cells counteract regulatory T-cell suppression through interleukin-6 and OX40/OX40L axis toward Th17-cell differentiation.

Blood

Piconese S,Gri G,Tripodo C,Musio S,Gorzanelli A,Frossi B,Pedotti R,Pucillo CE,Colombo MP

12-7061 was used in Flow cytometry/Cell sorting to show that mast cells, an early source of inflammatory mediators, are able to counteract Treg inhibition over effector T cells.

Thu Sep 24 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: B-cell hybridoma growth factor; H-IL-6; IL-6; ILN; Interleukin; Interleukin HP-1; Interleukin-6; Interleukin6; M-IL-6; R-IL-6

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Gene Aliases: Il-6; Il6

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UniProt ID: (Mouse) P08505

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Entrez Gene ID: (Mouse) 16193

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Function(s)

receptor binding cytokine activity interleukin-6 receptor binding protein binding growth factor activity

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IL-6 Monoclonal Antibody (MP5-20F3), PE, eBioscience™

IL-6 Antibody (12-7061-82) in Flow

Product Details

12-7061-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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