Invitrogen

IL-6 Monoclonal Antibody (MP5-32C11), Biotin, eBioscience™

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

7 Published Figures

23 References

View all (212) IL-6 antibodies

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Datasheet

Protocols

Questions & Answers

FIGURE: 1 / 8

IL-6 Antibody (13-7062-81) in ELISA

Journal of biomedical science 2021 - Fig. 6 5-MTP suppresses HFD-induced IL-6 and HSP60 in the atherosclerotic lesions of ApoE -/- mice. Chow diet-fed or HFD-fed ApoE -/- mice were treated with saline or L-5-MTP twice weekly for 20 wk. ( A , B ) IL-6 and ( C , D ) OPG in paraffin-embedded aortic tissues were analyzed by immunohistochemistry. Negative control represents staining with a 2nd antibody. Representative microphotographs of the immuno... View More

Product Image Gallery

IL-6 Antibody — Figure 4 Peli1 mediates LPS-induce inflammatory responses in microglia. ( a ) QPCR analysis of relative mRNA expression for the indicated inflammatory genes in the primary cultured microglia isolated from WT and Peli1 -KO mice, left untreated (NT) or stimulated with LPS (100 ng/ml). ( b ) ELISA of IL-1beta, IL-6, TNF and nitrites in the supernatant of WT and Peli1 -KO microglia cultures that left untreated (NT) or stimulated with LPS (100 ng/ml) at the indicated time points. ( c , d ) QPCR analysis of relative mRNA expression for the indicated inflammatory genes in the primary cultured microglia ( c ) or astrocytes ( d ) isolated from WT and Peli1 -KO mice, left untreated (NT) or stimulated with IL-1beta (20 ng/ml). The gene expression were normalized to a reference gene Actb (encoding beta-actin) and showed as the relative value to Actb in bar graphs. Data with error bars represent mean +- SD. Each panel is representative of at least three independent experiment. *P < 0.05, **P < 0.01 as determined by two-way ANOVA with a Bonferroni post test. {TM}

Product Details

13-7062-81

Applications

Tested Dilution

Publications

ELISA (ELISA)

0.5-2 µg/mL

View 22 publications 22 publications

Neutralization (Neu)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), Biotin, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MP5-32C11

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_466910

Product Specific Information

Description: The MP5-32C11 antibody reacts with mouse interleukin-6 (IL-6), a 21-28 kDa cytokine secreted by a variety of cell types.

Applications Reported: MP5-32C11 has been reported for use in detection of mouse IL-6 by ELISA and ELISPOT.

Applications Tested: The MP5-32C11 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of mouse Interleukin-6 (IL-6) in combination with the purified MP5-20F3 (14-7061) antibody for capture and recombinant mouse IL-6 (14-8061) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells. The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections. The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Metformin inhibition of mitochondrial ATP and DNA synthesis abrogates NLRP3 inflammasome activation and pulmonary inflammation.

Immunity

Xian H,Liu Y,Rundberg Nilsson A,Gatchalian R,Crother TR,Tourtellotte WG,Zhang Y,Aleman-Muench GR,Lewis G,Chen W,Kang S,Luevanos M,Trudler D,Lipton SA,Soroosh P,Teijaro J,de la Torre JC,Arditi M,Karin M,Sanchez-Lopez E

13-7062-81 was used in ELISA to conclude that inhibition of ATP and mtDNA synthesis is sufficient for ARDS amelioration.

Tue Jul 13 00:00:00 EDT 2021

Restoration of 5-methoxytryptophan protects against atherosclerotic chondrogenesis and calcification in ApoE^-/- mice fed high fat diet.

Journal of biomedical science

Lee GL,Liao TL,Wu JY,Wu KK,Kuo CC

Published figure using IL-6 monoclonal antibody (Product # 13-7062-81) in ELISA

Mon Nov 08 00:00:00 EST 2021

Murine Model of Sinusitis Infection for Screening Antimicrobial and Immunomodulatory Therapies.

Frontiers in cellular and infection microbiology

Alford MA,Choi KG,Trimble MJ,Masoudi H,Kalsi P,Pletzer D,Hancock REW

13-7062 was used in an ELISA assay to describe a simple less virulent, epidemic P. aeruginosa cystic fibrosis clinical isolate LESB65 model allowing non-invasive tracking of bacterial infection, and screening of host-responses to infection and therapies.

Fri Jul 02 00:00:00 EDT 2021

Murine Model of Sinusitis Infection for Screening Antimicrobial and Immunomodulatory Therapies.

Frontiers in cellular and infection microbiology

Alford MA,Choi KG,Trimble MJ,Masoudi H,Kalsi P,Pletzer D,Hancock REW

Fri Jul 02 00:00:00 EDT 2021

Microbiota Sensing by Mincle-Syk Axis in Dendritic Cells Regulates Interleukin-17 and -22 Production and Promotes Intestinal Barrier Integrity.

Immunity

Martínez-López M,Iborra S,Conde-Garrosa R,Mastrangelo A,Danne C,Mann ER,Reid DM,Gaboriau-Routhiau V,Chaparro M,Lorenzo MP,Minnerup L,Saz-Leal P,Slack E,Kemp B,Gisbert JP,Dzionek A,Robinson MJ,Rupérez FJ,Cerf-Bensussan N,Brown GD,Bernardo D,LeibundGut-Landmann S,Sancho D

13-7062-81 was used in ELISA to conclude that sensing of commensals by Mincle and Syk signaling in CD11c+ cells reinforces intestinal immune barrier and promotes host-microbiota mutualism, preventing systemic inflammation.

Tue Feb 19 00:00:00 EST 2019

Choline Uptake and Metabolism Modulate Macrophage IL-1β and IL-18 Production.

Cell metabolism

Sanchez-Lopez E,Zhong Z,Stubelius A,Sweeney SR,Booshehri LM,Antonucci L,Liu-Bryan R,Lodi A,Terkeltaub R,Lacal JC,Murphy AN,Hoffman HM,Tiziani S,Guma M,Karin M

13-7062 was used in ELISA to conclude that choline kinase inhibitors ameliorated acute and chronic models of IL-1β-dependent inflammation.

Tue Jun 04 00:00:00 EDT 2019

TLR2 Promotes Vascular Smooth Muscle Cell Chondrogenic Differentiation and Consequent Calcification via the Concerted Actions of Osteoprotegerin Suppression and IL-6-Mediated RANKL Induction.

Arteriosclerosis, thrombosis, and vascular biology

Lee GL,Yeh CC,Wu JY,Lin HC,Wang YF,Kuo YY,Hsieh YT,Hsu YJ,Kuo CC

13-7062-81 was used in ELISA to study the role of Toll-like receptor 2 in vascular calcification during atherosclerosis.

Fri Mar 01 00:00:00 EST 2019

Peli1 controls the survival of dopaminergic neurons through modulating microglia-mediated neuroinflammation.

Scientific reports

Dai D,Yuan J,Wang Y,Xu J,Mao C,Xiao Y

Published figure using IL-6 monoclonal antibody (Product # 13-7062-81) in ELISA

Wed May 29 00:00:00 EDT 2019

Vanadium Derivative Exposure Promotes Functional Alterations of VSMCs and Consequent Atherosclerosis via ROS/p38/NF-κB-Mediated IL-6 Production.

International journal of molecular sciences

Yeh CC,Wu JY,Lee GL,Wen HT,Lin P,Kuo CC

13-7062-81 was used in ELISA to conclude that NaVO3 exposure activates the ROS-triggering p38 signaling to selectively induce NF-κB-mediated IL-6 production.

Wed Dec 04 00:00:00 EST 2019

Vanadium Derivative Exposure Promotes Functional Alterations of VSMCs and Consequent Atherosclerosis via ROS/p38/NF-κB-Mediated IL-6 Production.

International journal of molecular sciences

Yeh CC,Wu JY,Lee GL,Wen HT,Lin P,Kuo CC

13-7062-81 was used in ELISA to conclude that NaVO3 exposure activates the ROS-triggering p38 signaling to selectively induce NF-κB-mediated IL-6 production.

Wed Dec 04 00:00:00 EST 2019

Peroxisomal β-oxidation regulates whole body metabolism, inflammatory vigor, and pathogenesis of nonalcoholic fatty liver disease.

JCI insight

Moreno-Fernandez ME,Giles DA,Stankiewicz TE,Sheridan R,Karns R,Cappelletti M,Lampe K,Mukherjee R,Sina C,Sallese A,Bridges JP,Hogan SP,Aronow BJ,Hoebe K,Divanovic S

Published figure using IL-6 monoclonal antibody (Product # 13-7062-81) in ELISA

Thu Mar 22 00:00:00 EDT 2018

Modulation of double-stranded RNA pattern recognition receptor signaling in ovarian cancer cells promotes inflammatory queues.

Oncotarget

Muccioli M,Nandigam H,Loftus T,Singh M,Venkatesh A,Wright J,Pate M,McCall K,Benencia F

13-7062 was used in an ELISA assay to investigate the potential of mouse and human ovarian cancer cells to produce inflammatory factors in response to pathogen recognition receptor signalling.

Fri Nov 30 00:00:00 EST 2018

Vitamin A differentially regulates cytokine expression in respiratory epithelial and macrophage cell lines.

Cytokine

Penkert RR,Jones BG,Häcker H,Partridge JF,Hurwitz JL

13-7062 was used in an ELISA assay to evaluate the cell specific effects of retinoic acid on PAMP triggered cytokine expression for LETs and macrophages in mice.

Wed Mar 01 00:00:00 EST 2017

Orally administered sodium 4-phenylbutyrate suppresses the development of dextran sulfate sodium-induced colitis in mice.

Experimental and therapeutic medicine

Ono K,Nimura S,Hideshima Y,Nabeshima K,Nakashima M

13-7062 was used in an ELISA assay to investigate the effects of orally administered sodium 4-phenylbutyrate in mice.

Fri Dec 01 00:00:00 EST 2017

K45A mutation of RIPK1 results in poor necroptosis and cytokine signaling in macrophages, which impacts inflammatory responses in vivo.

Cell death and differentiation

Shutinoski B,Alturki NA,Rijal D,Bertin J,Gough PJ,Schlossmacher MG,Sad S

13-7062 was used in an ELISA assay to investigate the impact of a K45A mutation of RIPK1 on necroptosis of macrophages and the activation of inflammatory response.

Sat Oct 01 00:00:00 EDT 2016

Nlrp12 mutation causes C57BL/6J strain-specific defect in neutrophil recruitment.

Nature communications

Ulland TK,Jain N,Hornick EE,Elliott EI,Clay GM,Sadler JJ,Mills KA,Janowski AM,Volk AP,Wang K,Legge KL,Gakhar L,Bourdi M,Ferguson PJ,Wilson ME,Cassel SL,Sutterwala FS

13-7062 was used in an ELISA assay to demonstrate that C57BL/6J mice have a functional defect in NLRP12, which is required by macrophages for effective neutrophil recruitment to inflammatory sites.

Tue Oct 25 00:00:00 EDT 2016

IL-6 and ICOS Antagonize Bim and Promote Regulatory T Cell Accrual with Age.

Journal of immunology (Baltimore, Md. : 1950)

Raynor J,Karns R,Almanan M,Li KP,Divanovic S,Chougnet CA,Hildeman DA

13706281 was used in ELISA to examine the accumulation of CD25 low T regulatory cells in aged mice

Sat Aug 01 00:00:00 EDT 2015

A new model of LMP1-MYC interaction in B cell lymphoma.

Leukemia & lymphoma

Ontiveros EP,Halwani A,Stunz LL,Kamberos N,Olivier AK,Janz S,Bishop GA

13-7062 was used in an ELISA assay to investigate cooperation between LMP1 and oncogenic MYC in B cell lymphoma.

Mon Dec 01 00:00:00 EST 2014

Immunoproteasome deficiency modifies the alternative pathway of NFκB signaling.

PloS one

Maldonado M,Kapphahn RJ,Terluk MR,Heuss ND,Yuan C,Gregerson DS,Ferrington DA

13-7062 was used in an ELISA assay to investigate whether the classical or alternative pathways are regulated by the immunoproteasome following chronic TNFα exposure.

Mon Aug 26 00:00:00 EDT 2013

The liver X receptor agonist GW3965 improves recovery from mild repetitive traumatic brain injury in mice partly through apolipoprotein E.

PloS one

Namjoshi DR,Martin G,Donkin J,Wilkinson A,Stukas S,Fan J,Carr M,Tabarestani S,Wuerth K,Hancock RE,Wellington CL

13-7062 was used in an ELISA assay to investigate the therapeutic use of Liver X receptor for treatment of TBI.

Tue Jul 09 00:00:00 EDT 2013

Type I IFN receptor and the B cell antigen receptor regulate TLR7 responses via distinct molecular mechanisms.

Journal of immunology (Baltimore, Md. : 1950)

Poovassery JS,Bishop GA

13-7062 was used in an ELISA assay to investigate the mechanisms that regulate TLR7 signals in the innate immune response, showing that Type I IFN receptor and the B cell antigen receptors are involved.

Wed Aug 15 00:00:00 EDT 2012

Nrf2 is essential for cholesterol crystal-induced inflammasome activation and exacerbation of atherosclerosis.

European journal of immunology

Freigang S,Ampenberger F,Spohn G,Heer S,Shamshiev AT,Kisielow J,Hersberger M,Yamamoto M,Bachmann MF,Kopf M

Fri Jul 01 00:00:00 EDT 2011

Berberine inhibits HIV protease inhibitor-induced inflammatory response by modulating ER stress signaling pathways in murine macrophages.

PloS one

Zha W,Liang G,Xiao J,Studer EJ,Hylemon PB,Pandak WM,Wang G,Li X,Zhou H

13-7062 was used in an ELISA assay to identify a key cellular mechanism underlying the protective effect of berberine on HIV PI-induced inflammatory response in macrophages.

Tue Feb 09 00:00:00 EST 2010

GM-CSF mediates autoimmunity by enhancing IL-6-dependent Th17 cell development and survival.

The Journal of experimental medicine

Sonderegger I,Iezzi G,Maier R,Schmitz N,Kurrer M,Kopf M

13-7062 was used in Blocking experiments to investigate how GM-CSF can promote generation and maintenance of Th17 cells by regulation of IL-6 and IL-23 in vivo.

Mon Sep 29 00:00:00 EDT 2008

TLR7 and CD40 cooperate in IL-6 production via enhanced JNK and AP-1 activation.

European journal of immunology

Vanden Bush TJ,Bishop GA

13-7062-81 was used in Enzyme-linked immunosorbent assay to demonstrate that CD40 and TLR7 dual stimulation of B cells enhances cytokine production through increased JNK signalling and AP-1 activity.

Fri Feb 01 00:00:00 EST 2008

Bioinformatics

Protein Aliases: B-cell hybridoma growth factor; H-IL-6; IL-6; ILN; Interleukin; Interleukin HP-1; Interleukin-6; Interleukin6; M-IL-6; R-IL-6

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Gene Aliases: Il-6; Il6

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UniProt ID: (Mouse) P08505

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Entrez Gene ID: (Mouse) 16193

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Function(s)

receptor binding cytokine activity interleukin-6 receptor binding protein binding growth factor activity

Process(es)

Performance Guarantee

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IL-6 Monoclonal Antibody (MP5-32C11), Biotin, eBioscience™

IL-6 Antibody (13-7062-81) in ELISA

Product Details

13-7062-81

ELISA (ELISA)

Neutralization (Neu)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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