Invitrogen

IL-6 Monoclonal Antibody (MQ2-13A5), PE, eBioscience™

5 Published Figures

14 References

View all (212) IL-6 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (5)

FIGURE: 1 / 6

IL-6 Antibody (12-7069-82) in Flow

Intracellular staining of normal human peripheral blood cells unstimulated (left) or stimulated with LPS and monensin (right) with Anti-Human CD14 PerCP-Cy5-5 (Product # 45-0149-42) and Anti-Human IL-6 PE. Cells in the monocyte gate were used for analysis.

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Product Details

12-7069-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.25 µg/test

View 13 publications 13 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Rat / IgG1, kappa

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MQ2-13A5

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_466168

Product Specific Information

Description: The MQ2-13A5 antibody reacts with human interleukin-6 (IL-6), a 21-28 kDa cytokine secreted by a variety of cell types.

Applications Reported:MQ2-13A5 has been reported for use in intracellular flow cytometric analysis.

Applications Tested: Has been tested by intracellular staining and flow cytometric analysis of stimulated human peripheral blood cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells. The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections. The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

BCG revaccination in adults enhances pro-inflammatory markers of trained immunity along with anti-inflammatory pathways.

iScience

Ahmed A,Tripathi H,van Meijgaarden KE,Kumar NC,Adiga V,Rakshit S,Parthiban C,Eveline J S,D'Souza G,Dias M,Ottenhoff THM,Netea MG,Joosten SA,Vyakarnam A

Published figure using IL-6 monoclonal antibody (Product # 12-7069-82) in Flow Cytometry

Fri Oct 20 00:00:00 EDT 2023

Evidence of SARS-CoV-2 infection in postmortem lung, kidney, and liver samples, revealing cellular targets involved in COVID-19 pathogenesis.

Archives of virology

Falcón-Cama V,Montero-González T,Acosta-Medina EF,Guillen-Nieto G,Berlanga-Acosta J,Fernández-Ortega C,Alfonso-Falcón A,Gilva-Rodríguez N,López-Nocedo L,Cremata-García D,Matos-Terrero M,Pentón-Rol G,Valdés I,Oramas-Díaz L,Suarez-Batista A,Noa-Romero E,Cruz-Sui O,Sánchez D,Borrego-Díaz AI,Valdés-Carreras JE,Vizcaino A,Suárez-Alba J,Valdés-Véliz R,Bergado G,González MA,Hernandez T,Alvarez-Arzola R,Ramírez-Suárez AC,Casillas-Casanova D,Lemos-Pérez G,Blanco-Águila OR,Díaz A,González Y,Bequet-Romero M,Marín-Prida J,Hernández-Perera JC,Del Rosario-Cruz L,Marin-Díaz AP,González-Bravo M,Borrajero I,Acosta-Rivero N

12-7069-82 was used in flow cytometry to identify the SARS-CoV-2 nucleocapsid (NC) in a variety of cells, colocalizing with mitochondrial proteins, lipid droplets (LDs), and key host proteins that have been implicated in inflammation, tissue repair, and the SARS-CoV-2 life cycle (vimentin, NLRP3, fibronectin, LC3B, DDX3X, and PPARγ), pointing to vimentin and LDs as platforms involved not only in the viral life cycle but also in inflammation and pathogenesis.

Sun Feb 26 00:00:00 EST 2023

Interleukin 6 regulates the expression of programmed cell death ligand 1 in thyroid cancer.

Cancer science

Zhang GQ,Jiao Q,Shen CT,Song HJ,Zhang HZ,Qiu ZL,Luo QY

Published figure using IL-6 monoclonal antibody (Product # 12-7069-82) in Flow Cytometry

Mon Mar 01 00:00:00 EST 2021

Myeloid cells protect intestinal epithelial barrier integrity through the angiogenin/plexin-B2 axis.

The EMBO journal

Bai R,Sun D,Chen M,Shi X,Luo L,Yao Z,Liu Y,Ge X,Gao X,Hu GF,Zhou W,Sheng J,Xu Z

12-7069-82 was used in Flow Cytometry to report that deficiency of angiogenin (Ang) in mouse myeloid cells caused impairment of epithelial barrier integrity, leading to high susceptibility to DSS-induced colitis.

Wed Jul 01 00:00:00 EDT 2020

CD11c⁺ B Cells Are Mainly Memory Cells, Precursors of Antibody Secreting Cells in Healthy Donors.

Frontiers in immunology

Golinski ML,Demeules M,Derambure C,Riou G,Maho-Vaillant M,Boyer O,Joly P,Calbo S

12-7069-82 was used in Flow Cytometry to characterise CD11c+ B cells from healthy donors by flow cytometry, microarray analysis, and in vitro functional assays.

Tue Feb 23 00:00:00 EST 2021

Plasmacytoid dendritic cell and myeloid dendritic cell function in ageing: A comparison between elderly and young adult women.

PloS one

Splunter MV,Perdijk O,Fick-Brinkhof H,Floris-Vollenbroek EG,Meijer B,Brugman S,Savelkoul HFJ,van Hoffen E,Joost van Neerven RJ

12-7069-82 was used in Flow Cytometry to confirm and extend the knowledge about immunosenescence and inflammageing on innate immunity in elderly women.

Wed Mar 25 00:00:00 EDT 2020

Biallelic hypomorphic mutations in a linear deubiquitinase define otulipenia, an early-onset autoinflammatory disease.

Proceedings of the National Academy of Sciences of the United States of America

Zhou Q,Yu X,Demirkaya E,Deuitch N,Stone D,Tsai WL,Kuehn HS,Wang H,Yang D,Park YH,Ombrello AK,Blake M,Romeo T,Remmers EF,Chae JJ,Mullikin JC,Güzel F,Milner JD,Boehm M,Rosenzweig SD,Gadina M,Welch SB,Özen S,Topaloglu R,Abinun M,Kastner DL,Aksentijevich I

Published figure using IL-6 monoclonal antibody (Product # 12-7069-82) in Flow Cytometry

Tue Sep 06 00:00:00 EDT 2016

Epigenetic regulation of HGK/MAP4K4 in T cells of type 2 diabetes patients.

Oncotarget

Chuang HC,Wang JS,Lee IT,Sheu WH,Tan TH

12-7069 was used in Flow cytometry/Cell sorting to identify HGK methylation/downregulation in T cells as a potential biomarker for non-obese type 2 diabetes.

Tue Mar 08 00:00:00 EST 2016

A microfluidic platform for high-throughput multiplexed protein quantitation.

PloS one

Volpetti F,Garcia-Cordero J,Maerkl SJ

12-7069 was used in Antibody array to test a novel device for quantifying biomarkers in multiple samples by immunoassay.

Fri Apr 29 00:00:00 EDT 2016

M2 Polarization of Monocytes-Macrophages Is a Hallmark of Indian Post Kala-Azar Dermal Leishmaniasis.

PLoS neglected tropical diseases

Mukhopadhyay D,Mukherjee S,Roy S,Dalton JE,Kundu S,Sarkar A,Das NK,Kaye PM,Chatterjee M

12-7069 was used in Flow cytometry/Cell sorting to investigate whether macrophages are deactivated or alternatively activated in Leishmania infection.

Thu Oct 01 00:00:00 EDT 2015

TL1A induces TCR independent IL-6 and TNF-α production and growth of PLZF⁺ leukocytes.

PloS one

Reichwald K,Jørgensen TZ,Tougaard P,Skov S

12-7069 was used in Flow cytometry/Cell sorting to investigate the role of the cytokine TL1A in autoimmune disease, showing that it induces the production of IL-6 and TNF-alpha, suggesting that it could be a possible target for the treatment of autoimmune disease.

Mon Sep 08 00:00:00 EDT 2014

Evaluation of suppressive and pro-resolving effects of EPA and DHA in human primary monocytes and T-helper cells.

Journal of lipid research

Jaudszus A,Gruen M,Watzl B,Ness C,Roth A,Lochner A,Barz D,Gabriel H,Rothe M,Jahreis G

12-7069 was used in Flow cytometry/Cell sorting to demonstrate that EPA and DHA do not generally affect immune cell functions in an inhibitory manner.

Mon Apr 01 00:00:00 EDT 2013

PD-1 negatively regulates interleukin-12 expression by limiting STAT-1 phosphorylation in monocytes/macrophages during chronic hepatitis C virus infection.

Immunology

Ma CJ,Ni L,Zhang Y,Zhang CL,Wu XY,Atia AN,Thayer P,Moorman JP,Yao ZQ

12-7069 was used in Flow cytometry/Cell sorting to show that the programmed death-1 inhibitory pathway may negatively regulate interleukin-12 expression by limiting STAT-1 phosphorylation in monocytes/macrophages during chronic hepatitis C virus infection.

Tue Mar 01 00:00:00 EST 2011

Enhanced neutrophil longevity and recruitment contribute to the severity of oviduct pathology during Chlamydia muridarum infection.

Infection and immunity

Frazer LC,O'Connell CM,Andrews CW,Zurenski MA,Darville T

12-7069 was used in Flow cytometry/Cell sorting to investigate the role of neutrophils in chlamydia-induced pathology.

Sat Oct 01 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: B-cell differentiation factor; B-cell stimulatory factor 2; BSF-2; CDF; CTL differentiation factor; H-IL-6; Hybridoma growth factor; IFN-beta-2; IL-6; ILN; Interferon beta-2; Interleukin; interleukin BSF-2; Interleukin-6; Interleukin6; M-IL-6; R-IL-6

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Gene Aliases: BSF-2; BSF2; CDF; HGF; HSF; IFN-beta-2; IFNB2; IL-6; IL6

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UniProt ID: (Human) P05231

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Entrez Gene ID: (Human) 3569

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Function(s)

cytokine activity interleukin-6 receptor binding protein binding growth factor activity

Process(es)

Performance Guarantee

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IL-6 Monoclonal Antibody (MQ2-13A5), PE, eBioscience™

IL-6 Antibody (12-7069-82) in Flow

Product Details

12-7069-82

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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