Invitrogen

IL-6 Monoclonal Antibody (MQ2-39C3), Biotin, eBioscience™

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

6 Published Figures

8 References

View all (212) IL-6 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Published Figures (6)
Advanced Verification (1)

FIGURE: 1 / 7

IL-6 Antibody (13-7068-81) in ELISA

Frontiers in cellular and infection microbiology 2021 - Figure 4 Host response to P. aeruginosa LESB strains in vitro . (A) Human bronchial epithelial cells (HBE) were infected with P. aeruginosa LESB58 or LESB65 at a multiplicity of infection (MOI) of 1, 5, 10, or 100 for 18 h. Amount of LDH in host-cell supernatants was quantified for determination of cytotoxicity. (B) At MOI = 10, P. aeruginosa LESB65 took 48 h to elicit cytotoxicity similar to that caused in... View More

Product Image Gallery

IL-6 Antibody — FIGURE 5. TLR blocking experiments. 200,000 human PBMC cells were stimulated with GMMA from different strains. After a 4-h incubation, IL-6 release was measured by ELISA and plotted as average of duplicates with standard deviation. A, IL-6 release after stimulation with 0.0001-1000 ng/ml (10-fold steps) of GMMA from different strains. The rectangle highlights the concentration of GMMA used in blocking experiments. B-E, cells were incubated with 25 mug/ml anti-TLR4 ( dark red graphs ), 15 mug/ml anti-TLR2 ( green graphs ), or both ( violet graphs ) for 30 min before exposure to 1 or 10 ng of GMMA from Ss -p - OAg Delta msbB ( B ); Sf 2a -p - OAg Delta msbB ( C ); Ss -p - OAg Delta htrB ( D ); and Sf 2a -p - OAg Delta htrB ( E ). The graphs from experiments without blocking are shown in the same color as in A . A representative result of three independent experiments is shown. {TM}

Product Details

13-7068-81

Applications

Tested Dilution

Publications

ELISA (ELISA)

0.5-2 µg/mL

View 6 publications 6 publications

Peptide Array (Array)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), Biotin, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MQ2-39C3

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_466912

Product Specific Information

Description: The MQ2-39C3 antibody reacts with human interleukin-6 (IL-6), a 21-28 kDa cytokine secreted by a variety of cell types.

Applications Reported: MQ2-39C3 has been reported for use in detection of human IL-6 by ELISA and immunoblotting (WB).

Applications Tested: The MQ2-39C3 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of human Interleukin-6 (IL-6) in combination with the purified MQ2-13A5 (14-7069) antibody for capture and recombinant human IL-6 (14-8069) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IL-6 is a gene that encodes a cytokine that plays a role in inflammation and the maturation of B cells. The protein produced by this gene is an endogenous pyrogen that can induce fever in people with autoimmune diseases or infections. The gene is primarily expressed at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Elevated levels of the encoded protein have been found in virus infections, including COVID-19 (disease caused by SARS-CoV-2). Diseases associated with IL6 include Kaposi Sarcoma and Rheumatoid Arthritis, Systemic Juvenile.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Murine Model of Sinusitis Infection for Screening Antimicrobial and Immunomodulatory Therapies.

Frontiers in cellular and infection microbiology

Alford MA,Choi KG,Trimble MJ,Masoudi H,Kalsi P,Pletzer D,Hancock REW

13-7068 was used in an ELISA assay to describe a simple less virulent, epidemic P. aeruginosa cystic fibrosis clinical isolate LESB65 model allowing non-invasive tracking of bacterial infection, and screening of host-responses to infection and therapies.

Fri Jul 02 00:00:00 EDT 2021

Murine Model of Sinusitis Infection for Screening Antimicrobial and Immunomodulatory Therapies.

Frontiers in cellular and infection microbiology

Alford MA,Choi KG,Trimble MJ,Masoudi H,Kalsi P,Pletzer D,Hancock REW

Fri Jul 02 00:00:00 EDT 2021

TREM2 Alzheimer's variant R47H causes similar transcriptional dysregulation to knockout, yet only subtle functional phenotypes in human iPSC-derived macrophages.

Alzheimer's research & therapy

Hall-Roberts H,Agarwal D,Obst J,Smith TB,Monzón-Sandoval J,Di Daniel E,Webber C,James WS,Mead E,Davis JB,Cowley SA

13-7068 was used in an ELISA assay to investigated cell phenotypes of the R47H mutant and TREM2 KO in a model of human microglia, and compared their transcriptional signatures, to determine the mechanism by which R47H TREM2 disrupts function.

Mon Nov 16 00:00:00 EST 2020

Multiplatform Biomarker Discovery for Bladder Cancer Recurrence Diagnosis.

Disease markers

De Paoli M,Gogalic S,Sauer U,Preininger C,Pandha H,Simpson G,Horvath A,Marquette C

13-7068 was used in Antibody array to select a multiparameter panel, comprising urinary biomarkers and clinical parameters, for nonmuscle invasive bladder cancer recurrence diagnosis.

Wed Nov 20 00:00:00 EST 2019

Toll-Like Receptor Activation by Generalized Modules for Membrane Antigens from Lipid A Mutants of Salmonella enterica Serovars Typhimurium and Enteritidis.

Clinical and vaccine immunology : CVI

Rossi O,Caboni M,Negrea A,Necchi F,Alfini R,Micoli F,Saul A,MacLennan CA,Rondini S,Gerke C

13-7068 was used in an ELISA assay to investigate the differences between Salmonella enterica strains in terms of TLR activation by generalised modules for membrane antigens from lipid A mutants.

Fri Apr 01 00:00:00 EDT 2016

Development and validation of panoptic Meso scale discovery assay to quantify total systemic interleukin-6.

British journal of clinical pharmacology

Chaturvedi S,Siegel D,Wagner CL,Park J,van de Velde H,Vermeulen J,Fung MC,Reddy M,Hall B,Sasser K

Published figure using IL-6 monoclonal antibody (Product # 13-7068-81) in ELISA

Thu Oct 01 00:00:00 EDT 2015

A microfluidic platform for high-throughput multiplexed protein quantitation.

PloS one

Volpetti F,Garcia-Cordero J,Maerkl SJ

13-7068 was used in Antibody array to test a novel device for quantifying biomarkers in multiple samples by immunoassay.

Fri Apr 29 00:00:00 EDT 2016

Modulation of endotoxicity of Shigella generalized modules for membrane antigens (GMMA) by genetic lipid A modifications: relative activation of TLR4 and TLR2 pathways in different mutants.

The Journal of biological chemistry

Rossi O,Pesce I,Giannelli C,Aprea S,Caboni M,Citiulo F,Valentini S,Ferlenghi I,MacLennan CA,D'Oro U,Saul A,Gerke C

13-7068 was used in an ELISA assay to investigate the use of gram-negative bacteria as vaccine candidates.

Fri Sep 05 00:00:00 EDT 2014

Induction of cytokines in a macrophage cell line by proteins of Clostridium difficile.

FEMS immunology and medical microbiology

Vohra P,Poxton IR

13-7068 was used in an ELISA assay to assess the induction of cytokines in a macrophage cell line in response to different antigens prepared from five Clostridium difficile strains.

Fri Jun 01 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: B-cell differentiation factor; B-cell stimulatory factor 2; BSF-2; CDF; CTL differentiation factor; H-IL-6; Hybridoma growth factor; IFN-beta-2; IL-6; ILN; Interferon beta-2; Interleukin; interleukin BSF-2; Interleukin-6; Interleukin6; M-IL-6; R-IL-6

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Gene Aliases: BSF-2; BSF2; CDF; HGF; HSF; IFN-beta-2; IFNB2; IL-6; IL6

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UniProt ID: (Human) P05231

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Entrez Gene ID: (Human) 3569

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Function(s)

cytokine activity interleukin-6 receptor binding protein binding growth factor activity

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IL-6 Monoclonal Antibody (MQ2-39C3), Biotin, eBioscience™

IL-6 Antibody (13-7068-81) in ELISA

Product Details

13-7068-81

ELISA (ELISA)

Peptide Array (Array)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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