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IL-7 Antibody (500-P310BT-25UG) in WB
Western Blot: To detect Rat IL-7 by Western Blot analysis IL-7 Polyclonal Antibody, Biotin (Product # 500-P310BT-1MG) can be used at a concentration of 0.1-0.2 mg/mL. When used in conjunction with compatible development reagents the detection limit for Recombinant Rat IL-7 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P310BT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Rat
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Rat IL-7
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2930333
Product Specific Information
AA Sequence of recombinant protein: MDCHIKDKDG KAFGSVLMIS INQLDKMTGT DSDCPNNEPN FFKKHLCDDT KEAAFLNRAA RKLRQFLKMN ISEEFNDHLL RVSDGTQTLV NCTSKEEKTI KEQKKNDPCF LKRLLREIKT CWNKILKGSI.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Rat IL-7. Anti-Rat IL-7-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Rat IL-7 by sandwich ELISA (using 100 mL/well) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Rat IL-7 (500-P310) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Rat IL-7.
Western Blot: To detect Rat IL-7 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 mg/mL. When used in conjunction with compatible development reagents the detection limit for Recombinant Rat IL-7 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P310BT-1MG will be provided as 2 x 500 µg
Target Information
Interleukin 7 (IL-7) is a hematopoietic cytokine that is constitutively produced by stromal cells from the bone marrow and thymus. IL-7 signals through the IL-7R to promote the differentiation of hematopoietic stem cells to lymphoid precursor cells, which give rise to T cells, B cells, and natural killer cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: H-IL-7; IL-7; ILN; Interleukin; interleukin-7; interleukin-7 receptor subunit alpha; Interleukin7; M-IL-7
Entrez Gene ID: (Rat) 25647, (Rat) 294797
T cell lineage commitment
immune response
humoral immune response
cell-cell signaling
organ morphogenesis
regulation of gene expression
positive regulation of B cell proliferation
negative regulation of apoptotic process
negative regulation of catalytic activity
bone resorption
positive regulation of T cell differentiation
positive regulation of organ growth
homeostasis of number of cells within a tissue
negative regulation of extrinsic apoptotic signaling pathway
negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
cell morphogenesis
negative regulation of T cell mediated cytotoxicity
immunoglobulin production
biological_process
regulation of cell size
positive regulation of gene expression
cell growth
cytokine-mediated signaling pathway
T cell differentiation
positive regulation of T cell differentiation in thymus
B cell proliferation
lymph node development
homeostasis of number of cells
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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