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IL-7 Antibody (500-P57-1MG) in WB
Western Blot: To detect Murine IL-7 by Western Blot analysis IL-7 Polyclonal Antibody (Product # 500-P57-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Murine IL-7 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P57-1MG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Murine IL-7
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2930636
Product Specific Information
AA Sequence of recombinant protein: MECHIKDKEG KAYESVLMIS IDELDKMTGT DSNCPNNEPN FFRKHVCDDT KEAAFLNRAA RKLKQFLKMN ISEEFNVHLL TVSQGTQTLV NCTSKEEKNV KEQKKNDACF LKRLLREIKT CWNKILKGSI.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine IL-7. Anti-Murine IL-7-specific antibody was purified by affinity chromatography employing an immobilized Murine IL-7 matrix.
Sandwich ELISA: To detect Murine IL-7 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine IL-7 (500-P57Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine IL-7.
Western Blot: To detect Murine IL-7 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Murine IL-7 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
Interleukin 7 (IL-7) is a hematopoietic cytokine that is constitutively produced by stromal cells from the bone marrow and thymus. IL-7 signals through the IL-7R to promote the differentiation of hematopoietic stem cells to lymphoid precursor cells, which give rise to T cells, B cells, and natural killer cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD127; H-IL-7; IL-7; IL-7 receptor alpha chain; IL-7 receptor subunit alpha; IL-7R subunit alpha; IL-7R-alpha; IL-7RA; ILN; Interleukin; interleukin 7 receptor alpha chain; Interleukin-7; Interleukin-7 receptor subunit alpha; Interleukin7; M-IL-7
Gene Aliases: A630026I06Rik; CD127; hlb368; Il-7; IL-7Ralpha; Il7; Il7r
UniProt ID: (Mouse) P10168, (Mouse) P16872
Entrez Gene ID: (Mouse) 16196, (Mouse) 16197
T cell lineage commitment
immune response
humoral immune response
cell-cell signaling
organ morphogenesis
regulation of gene expression
positive regulation of B cell proliferation
negative regulation of catalytic activity
bone resorption
positive regulation of T cell differentiation
positive regulation of organ growth
homeostasis of number of cells within a tissue
negative regulation of extrinsic apoptotic signaling pathway
negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
cell morphogenesis
negative regulation of T cell mediated cytotoxicity
immunoglobulin production
regulation of cell size
positive regulation of gene expression
cell growth
T cell differentiation
positive regulation of T cell differentiation in thymus
B cell proliferation
lymph node development
homeostasis of number of cells
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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