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Invitrogen
IL-7 Polyclonal Antibody
Advanced Verification
This Antibody was verified by Neutralization to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 3
IL-7 Antibody (PA5-46944) in Neu
Neutralization of IL-7 in PHA-activated human peripheral blood mononuclear cells (PBMC). Samples were incubated in IL-7 polyclonal antibody (Product # PA5-46944). Recombinant Mouse IL‚7 stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‚7 (1.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL‚7 Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 0.2-0.4 µg/mL.
Product Details
PA5-46944
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Not Applicable
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived recombinant mouse IL-7 Glu26-Ile154
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2609533
Product Specific Information
In sandwich ELISAs, approximately 20% cross-reactivity with recombinant rat IL-7 is observed, and less than 0.1% cross-reactivity with recombinant human IL-7 is observed. This antibody neutralizes the biological activity of recombinant mouse IL-7. This antibody will not neutralize the biological activity of recombinant human IL-7.
Reconstitute at 0.2 mg/mL in sterile PBS.
Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.
Target Information
Interleukin 7 (IL-7) is a hematopoietic cytokine that is constitutively produced by stromal cells from the bone marrow and thymus. IL-7 signals through the IL-7R to promote the differentiation of hematopoietic stem cells to lymphoid precursor cells, which give rise to T cells, B cells, and natural killer cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD127; H-IL-7; IL-7; IL-7 receptor alpha chain; IL-7 receptor subunit alpha; IL-7R subunit alpha; IL-7R-alpha; IL-7RA; ILN; Interleukin; interleukin 7 receptor alpha chain; Interleukin-7; Interleukin-7 receptor subunit alpha; Interleukin7; M-IL-7
Gene Aliases: A630026I06Rik; CD127; hlb368; Il-7; IL-7Ralpha; Il7; Il7r
UniProt ID: (Mouse) P10168, (Mouse) P16872
Entrez Gene ID: (Mouse) 16196, (Mouse) 16197
T cell lineage commitment
immune response
humoral immune response
cell-cell signaling
organ morphogenesis
regulation of gene expression
positive regulation of B cell proliferation
negative regulation of catalytic activity
bone resorption
positive regulation of T cell differentiation
positive regulation of organ growth
homeostasis of number of cells within a tissue
negative regulation of extrinsic apoptotic signaling pathway
negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
cell morphogenesis
negative regulation of T cell mediated cytotoxicity
immunoglobulin production
regulation of cell size
positive regulation of gene expression
cell growth
T cell differentiation
positive regulation of T cell differentiation in thymus
B cell proliferation
lymph node development
homeostasis of number of cells
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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