Invitrogen

IL-8 (CXCL8) Polyclonal Antibody, PeproTech®

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IL-8 (CXCL8) Antibody (500-P28-1MG) in ELISA

Sandwich ELISA: To detect Human IL-8 (CXCL8) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of IL-8 (CXCL8) Polyclonal Antibody (Product # 500-P28-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech IL-8 (CXCL8) Polyclonal Antibody, Biotin (Product # 500-P28BT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IL-8 (CXCL8).

IL-8 (CXCL8) Antibody in Western Blot (WB)

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Product Details

500-P28-1MG

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.2 µg/mL

View 2 publications 2 publications

ELISA (ELISA)

0.5-2.0 µg/mL

View 9 publications 9 publications

Immunoprecipitation (IP)

View 1 publication 1 publication

Neutralization (Neu)

View 3 publications 3 publications

Blocking Assay (BLOCK)

View 1 publication 1 publication

In vitro Assay (IV)

View 7 publications 7 publications

Immunostaining (IS)

View 4 publications 4 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Human IL-8 (CXCL8) (72 a.a.)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2930234

Product Specific Information

AA Sequence of recombinant protein: SAKELRCQCI KTYSKPFHPK FIKELRVIES GPHCANTEII VKLSDGRELC LDPKENWVQR VVEKFLKRAE NS.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IL-8 (CXCL8). Anti-Human IL-8 (CXCL8)-specific antibody was purified by affinity chromatography employing an immobilized Human IL-8 (CXCL8) matrix.

Sandwich ELISA: To detect Human IL-8 (CXCL8) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human IL-8 (CXCL8) (500-P28Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IL-8 (CXCL8).

Western Blot: To detect Human IL-8 (CXCL8) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human IL-8 (CXCL8) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

Interleukin 8 (IL-8, CXCL8) is a 72 amino acid pro-inflammatory factor which belongs to the CXC subfamily of chemokines, and are bound by the cell surface receptors IL-8RA and IL-8RB. IL-8 functions as a chemoattractant and potent angiogenic factor. The expression and secretion of IL-8 can be induced by diverse inflammatory stimuli in many cells, including macrophages and endothelial cells. In endothelial cells, IL-8 is present in storage vesicles called Weibel-Palade bodies. IL-8, first isolated from osteosarcoma cells, contains the ELR-motif (N-terminal Glu-Leu-Arg amino acid sequence) and signals through the CXCR1 and CXCR2 receptors. Previous nomenclature for IL-8 includes neutrophil activating protein 1 (NAP-1), granulocyte chemotactic protein 1 (GCP-1), monocyte-derived neutrophil-activating peptide (MONAP) and protein 3-10C. IL-8 and ten other members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q. Cancer studies have demonstrated a role for IL-8 in the angiogenesis and growth of tumours, and IL-8 is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Tumor Cell-Autonomous Pro-Metastatic Activities of PD-L1 in Human Breast Cancer Are Mediated by PD-L1-S283 and Chemokine Axes.

Cancers

Erlichman N,Baram T,Meshel T,Morein D,Da'adoosh B,Ben-Baruch A

500-P28 was used in Enzyme-linked immunosorbent assay to investigate the role of WT-PD-L1 in triple-negative breast cancer.

Fri Feb 18 00:00:00 EST 2022

PLAU directs conversion of fibroblasts to inflammatory cancer-associated fibroblasts, promoting esophageal squamous cell carcinoma progression via uPAR/Akt/NF-κB/IL8 pathway.

Cell death discovery

Fang L,Che Y,Zhang C,Huang J,Lei Y,Lu Z,Sun N,He J

Thu Feb 11 00:00:00 EST 2021

Notch-Mediated Tumor-Stroma-Inflammation Networks Promote Invasive Properties and CXCL8 Expression in Triple-Negative Breast Cancer.

Frontiers in immunology

Liubomirski Y,Lerrer S,Meshel T,Morein D,Rubinstein-Achiasaf L,Sprinzak D,Wiemann S,Körner C,Ehrlich M,Ben-Baruch A

500-P28 was used in Enzyme-linked immunosorbent assay to investigate pro-metastatic chemokines in triple-negative breast cancer.

Thu Oct 01 00:00:00 EDT 2020

Tumor-Stroma-Inflammation Networks Promote Pro-metastatic Chemokines and Aggressiveness Characteristics in Triple-Negative Breast Cancer.

Frontiers in immunology

Liubomirski Y,Lerrer S,Meshel T,Rubinstein-Achiasaf L,Morein D,Wiemann S,Körner C,Ben-Baruch A

500-P28 was used in Enzyme-linked immunosorbent assay to study the impact of tumor-stroma-inflammation networks on pro-metastatic phenotypes in triple-negative breast cancer (TNBC) to identify a novel tumor-stroma-inflammation networks that may promote TNBC aggressiveness by increasing the pro-malignancy potential of the TME and of the tumor cells themselves, and reveal key roles for CXCL8 in mediating these metastasis-promoting activities.

Mon Aug 10 00:00:00 EDT 2020

Co-Inflammatory Roles of TGFβ1 in the Presence of TNFα Drive a Pro-inflammatory Fate in Mesenchymal Stem Cells.

Frontiers in immunology

Lerrer S,Liubomirski Y,Bott A,Abnaof K,Oren N,Yousaf A,Körner C,Meshel T,Wiemann S,Ben-Baruch A

500-P28 was used in Enzyme-linked immunosorbent assay to demonstrate a TNFα + TGFβ1-driven pro-inflammatory fate in MSCs, identify specific molecular mechanisms involved, and propose that TNFα + TGFβ1-stimulated MSCs influence the tumor niche.

Wed Nov 20 00:00:00 EST 2019

The cerebral cavernous malformation 3 gene is necessary for senescence induction.

Aging cell

Guerrero A,Iglesias C,Raguz S,Floridia E,Gil J,Pombo CM,Zalvide J

Wed Apr 01 00:00:00 EDT 2015

The cerebral cavernous malformation 3 gene is necessary for senescence induction.

Aging cell

Guerrero A,Iglesias C,Raguz S,Floridia E,Gil J,Pombo CM,Zalvide J

Wed Apr 01 00:00:00 EDT 2015

The cerebral cavernous malformation 3 gene is necessary for senescence induction.

Aging cell

Guerrero A,Iglesias C,Raguz S,Floridia E,Gil J,Pombo CM,Zalvide J

Wed Apr 01 00:00:00 EDT 2015

Local delivery of VEGF and SDF enhances endothelial progenitor cell recruitment and resultant recovery from ischemia.

Tissue engineering. Part A

Anderson EM,Kwee BJ,Lewin SA,Raimondo T,Mehta M,Mooney DJ

500-P28 was used in Blocking experiments to demonstrate that accumulation of infused endothelial progenitors can be enriched using biomaterial-based delivery of VEGF and SDF.

Wed Apr 01 00:00:00 EDT 2015

Regulation of the inflammatory profile of stromal cells in human breast cancer: prominent roles for TNF-α and the NF-κB pathway.

Stem cell research & therapy

Katanov C,Lerrer S,Liubomirski Y,Leider-Trejo L,Meshel T,Bar J,Feniger-Barish R,Kamer I,Soria-Artzi G,Kahani H,Banerjee D,Ben-Baruch A

500-P28 was used in Enzyme-linked immunosorbent assay to study the impact of tumour necrosis factor α and interleukin 1β on the inflammatory phenotype of cancer-associated fibroblasts and mesenchymal stem cells.

Fri May 01 00:00:00 EDT 2015

Tumor microenvironment B cells increase bladder cancer metastasis via modulation of the IL-8/androgen receptor (AR)/MMPs signals.

Oncotarget

Ou Z,Wang Y,Liu L,Li L,Yeh S,Qi L,Chang C

500-P28 was used in Neutralisation experiments to study the effects of B cells on bladder cancer cell invasion via IL-8/androgen receptor signals, and show that blocking the IL-8/AR/MMPs signals partially reversed the infiltrating B cells capacity to increase the bladder cancer cell invasion.

Tue Sep 22 00:00:00 EDT 2015

The inflammatory cytokine TNFα cooperates with Ras in elevating metastasis and turns WT-Ras to a tumor-promoting entity in MCF-7 cells.

BMC cancer

Leibovich-Rivkin T,Liubomirski Y,Meshel T,Abashidze A,Brisker D,Solomon H,Rotter V,Weil M,Ben-Baruch A

500-P28 was used in Enzyme-linked immunosorbent assay to determine the relative contribution of two processes to breast cancer progression: (1) Intrinsic events, such as activation of the Ras pathway and down-regulation of p53; (2) The inflammatory cytokines TNFα and IL-1β, shown in our published studies to be highly expressed in tumors of >80% of breast cancer patients with recurrent disease.

Thu Mar 06 00:00:00 EST 2014

Progression of luminal breast tumors is promoted by ménage à trois between the inflammatory cytokine TNFα and the hormonal and growth-supporting arms of the tumor microenvironment.

Mediators of inflammation

Weitzenfeld P,Meron N,Leibovich-Rivkin T,Meshel T,Ben-Baruch A

500-P28 was used in Enzyme-linked immunosorbent assay to indicate that TNF α can turn into a strong prometastatic factor, suggesting a paradigm shift in which clinically approved inhibitors of TNFα would be applied in breast cancer therapy.

Thu Jun 05 00:00:00 EDT 2014

Inflammatory factors of the tumor microenvironment induce plasticity in nontransformed breast epithelial cells: EMT, invasion, and collapse of normally organized breast textures.

Neoplasia (New York, N.Y.)

Leibovich-Rivkin T,Liubomirski Y,Bernstein B,Meshel T,Ben-Baruch A

500-P28 was used in Enzyme-linked immunosorbent assay to reveal novel pathways through which the inflammatory microenvironment may contribute to relapsed disease in breast cancer.

Sun Dec 01 00:00:00 EST 2013

Spindle shaped human mesenchymal stem/stromal cells from amniotic fluid promote neovascularization.

PloS one

Roubelakis MG,Tsaknakis G,Pappa KI,Anagnou NP,Watt SM

Fri Sep 06 00:00:00 EDT 2013

Glioblastoma cell-secreted interleukin-8 induces brain endothelial cell permeability via CXCR2.

PloS one

Dwyer J,Hebda JK,Le Guelte A,Galan-Moya EM,Smith SS,Azzi S,Bidere N,Gavard J

Wed Mar 06 00:00:00 EST 2013

Glioblastoma cell-secreted interleukin-8 induces brain endothelial cell permeability via CXCR2.

PloS one

Dwyer J,Hebda JK,Le Guelte A,Galan-Moya EM,Smith SS,Azzi S,Bidere N,Gavard J

Wed Mar 06 00:00:00 EST 2013

Disease-associated GPR56 mutations cause bilateral frontoparietal polymicrogyria via multiple mechanisms.

The Journal of biological chemistry

Chiang NY,Hsiao CC,Huang YS,Chen HY,Hsieh IJ,Chang GW,Lin HH

Fri Apr 22 00:00:00 EDT 2011

Predominant expression of CCL2 at the tumor site of prostate cancer patients directs a selective loss of immunological tolerance to CCL2 that could be amplified in a beneficial manner.

Journal of immunology (Baltimore, Md. : 1950)

Izhak L,Wildbaum G,Weinberg U,Shaked Y,Alami J,Dumont D,Friedman B,Stein A,Karin N

Fri Jan 15 00:00:00 EST 2010

Trans- but not cis-resveratrol impairs angiotensin-II-mediated vascular inflammation through inhibition of NF-κB activation and peroxisome proliferator-activated receptor-gamma upregulation.

Journal of immunology (Baltimore, Md. : 1950)

Rius C,Abu-Taha M,Hermenegildo C,Piqueras L,Cerda-Nicolas JM,Issekutz AC,Estañ L,Cortijo J,Morcillo EJ,Orallo F,Sanz MJ

Wed Sep 15 00:00:00 EDT 2010

Roles for the human ATP-dependent Lon protease in mitochondrial DNA maintenance.

The Journal of biological chemistry

Lu B,Yadav S,Shah PG,Liu T,Tian B,Pukszta S,Villaluna N,Kutejová E,Newlon CS,Santos JH,Suzuki CK

Fri Jun 15 00:00:00 EDT 2007

Generation and growth of CD28nullCD8+ memory T cells mediated by IL-15 and its induced cytokines.

Journal of immunology (Baltimore, Md. : 1950)

Chiu WK,Fann M,Weng NP

Fri Dec 01 00:00:00 EST 2006

Generation and growth of CD28nullCD8+ memory T cells mediated by IL-15 and its induced cytokines.

Journal of immunology (Baltimore, Md. : 1950)

Chiu WK,Fann M,Weng NP

Fri Dec 01 00:00:00 EST 2006

The role of CXCR2/CXCR2 ligand biological axis in renal cell carcinoma.

Journal of immunology (Baltimore, Md. : 1950)

Mestas J,Burdick MD,Reckamp K,Pantuck A,Figlin RA,Strieter RM

Sat Oct 15 00:00:00 EDT 2005

Characterization of a novel chemokine-containing storage granule in endothelial cells: evidence for preferential exocytosis mediated by protein kinase A and diacylglycerol.

Journal of immunology (Baltimore, Md. : 1950)

Øynebråten I,Barois N,Hagelsteen K,Johansen FE,Bakke O,Haraldsen G

Sat Oct 15 00:00:00 EDT 2005

Cytolytically inactive terminal complement complex causes transendothelial migration of polymorphonuclear leukocytes in vitro and in vivo.

Blood

Dobrina A,Pausa M,Fischetti F,Bulla R,Vecile E,Ferrero E,Mantovani A,Tedesco F

Tue Jan 01 00:00:00 EST 2002

Fibrin regulation of interleukin-8 gene expression in human vascular endothelial cells.

Blood

Qi J,Goralnick S,Kreutzer DL

Sat Nov 01 00:00:00 EST 1997

Bioinformatics

Protein Aliases: alveolar macrophage chemotactic factor I; ANX7; beta endothelial cell-derived neutrophil activating peptide; beta-thromboglobulin-like protein; C-X-C motif chemokine 8; Chemokine (C-X-C motif) ligand 8; Emoctakin; GCP-1; Granulocyte chemotactic protein 1; IL-8; ILN; Interleukin; interleukin 8; Interleukin-8; Interleukin8; lung giant cell carcinoma-derived chemotactic protein; lymphocyte derived neutrophil activating peptide; MDNCF; MONAP; Monocyte-derived neutrophil chemotactic factor; Monocyte-derived neutrophil-activating peptide; NAP-1; neutrophil-activating peptide 1; Neutrophil-activating protein 1; Protein 3-10C; RP11-537A6.8; small inducible cytokine subfamily B, member 8; SNX; SYNEXIN; T-cell chemotactic factor; tumor necrosis factor-induced gene 1

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Gene Aliases: CXCL8; GCP-1; GCP1; IL8; LECT; LUCT; LYNAP; MDNCF; MONAP; NAF; NAP-1; NAP1

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UniProt ID: (Human) P10145

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Entrez Gene ID: (Human) 3576

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Function(s)

interleukin-8 receptor binding protein binding chemokine activity chemokine

Process(es)

angiogenesis response to molecule of bacterial origin movement of cell or subcellular component chemotaxis inflammatory response immune response cell cycle arrest signal transduction G-protein coupled receptor signaling pathway negative regulation of cell proliferation calcium-mediated signaling regulation of cell adhesion neutrophil chemotaxis receptor internalization response to endoplasmic reticulum stress intracellular signal transduction PERK-mediated unfolded protein response neutrophil activation cellular response to fibroblast growth factor stimulus regulation of single stranded viral RNA replication via double stranded DNA intermediate negative regulation of G-protein coupled receptor protein signaling pathway positive regulation of angiogenesis embryonic digestive tract development induction of positive chemotaxis chemokine-mediated signaling pathway cellular response to lipopolysaccharide cellular response to interleukin-1 cellular response to tumor necrosis factor positive regulation of neutrophil chemotaxis

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IL-8 (CXCL8) Polyclonal Antibody, PeproTech®

IL-8 (CXCL8) Antibody (500-P28-1MG) in ELISA

Product Details

500-P28-1MG

Western Blot (WB)

ELISA (ELISA)

Immunoprecipitation (IP)

Neutralization (Neu)

Blocking Assay (BLOCK)

In vitro Assay (IV)

Immunostaining (IS)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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