Invitrogen

IL-8 (CXCL8) Polyclonal Antibody, Biotin, PeproTech®

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IL-8 (CXCL8) Antibody (500-P28BT-25UG) in ELISA

Sandwich ELISA: To detect Human IL-8 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of IL-8 (CXCL8) Polyclonal Antibody, Biotin (Product # 500-P28BT-1MG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech IL-8 (CXCL8) Polyclonal Antibody (Product # 500-P28-1MG) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IL-8.

IL-8 (CXCL8) Antibody in Western Blot (WB)

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Product Details

500-P28BT-25UG

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.2 µg/mL

ELISA (ELISA)

0.25-1.0 µg/mL

View 12 publications 12 publications

In vitro Assay (IV)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Human IL-8 (CXCL8) (72 a.a.)

View immunogen

Conjugate

Biotin Biotin Biotin

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2930255

Product Specific Information

AA Sequence of recombinant protein: SAKELRCQCI KTYSKPFHPK FIKELRVIES GPHCANTEII VKLSDGRELC LDPKENWVQR VVEKFLKRAE NS.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IL-8 (CXCL8). Anti-Human IL-8 (CXCL8)-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA: To detect Human IL-8 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human IL-8 (500-P28) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IL-8.

Western Blot: To detect Human IL-8 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human IL-8 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

500-P28BT-1MG will be provided as 2 x 500 µg

Target Information

Interleukin 8 (IL-8, CXCL8) is a 72 amino acid pro-inflammatory factor which belongs to the CXC subfamily of chemokines, and are bound by the cell surface receptors IL-8RA and IL-8RB. IL-8 functions as a chemoattractant and potent angiogenic factor. The expression and secretion of IL-8 can be induced by diverse inflammatory stimuli in many cells, including macrophages and endothelial cells. In endothelial cells, IL-8 is present in storage vesicles called Weibel-Palade bodies. IL-8, first isolated from osteosarcoma cells, contains the ELR-motif (N-terminal Glu-Leu-Arg amino acid sequence) and signals through the CXCR1 and CXCR2 receptors. Previous nomenclature for IL-8 includes neutrophil activating protein 1 (NAP-1), granulocyte chemotactic protein 1 (GCP-1), monocyte-derived neutrophil-activating peptide (MONAP) and protein 3-10C. IL-8 and ten other members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q. Cancer studies have demonstrated a role for IL-8 in the angiogenesis and growth of tumours, and IL-8 is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Tumor Cell-Autonomous Pro-Metastatic Activities of PD-L1 in Human Breast Cancer Are Mediated by PD-L1-S283 and Chemokine Axes.

Cancers

Erlichman N,Baram T,Meshel T,Morein D,Da'adoosh B,Ben-Baruch A

500-P28Bt was used in Enzyme-linked immunosorbent assay to investigate the role of WT-PD-L1 in triple-negative breast cancer.

Fri Feb 18 00:00:00 EST 2022

Notch-Mediated Tumor-Stroma-Inflammation Networks Promote Invasive Properties and CXCL8 Expression in Triple-Negative Breast Cancer.

Frontiers in immunology

Liubomirski Y,Lerrer S,Meshel T,Morein D,Rubinstein-Achiasaf L,Sprinzak D,Wiemann S,Körner C,Ehrlich M,Ben-Baruch A

500-P28Bt was used in Enzyme-linked immunosorbent assay to investigate pro-metastatic chemokines in triple-negative breast cancer.

Thu Oct 01 00:00:00 EDT 2020

Tumor-Stroma-Inflammation Networks Promote Pro-metastatic Chemokines and Aggressiveness Characteristics in Triple-Negative Breast Cancer.

Frontiers in immunology

Liubomirski Y,Lerrer S,Meshel T,Rubinstein-Achiasaf L,Morein D,Wiemann S,Körner C,Ben-Baruch A

500-P28Bt was used in Enzyme-linked immunosorbent assay to study the impact of tumor-stroma-inflammation networks on pro-metastatic phenotypes in triple-negative breast cancer (TNBC) to identify a novel tumor-stroma-inflammation networks that may promote TNBC aggressiveness by increasing the pro-malignancy potential of the TME and of the tumor cells themselves, and reveal key roles for CXCL8 in mediating these metastasis-promoting activities.

Mon Aug 10 00:00:00 EDT 2020

Novel Anti-Inflammatory Peptides Based on Chemokine-Glycosaminoglycan Interactions Reduce Leukocyte Migration and Disease Severity in a Model of Rheumatoid Arthritis.

Journal of immunology (Baltimore, Md. : 1950)

McNaughton EF,Eustace AD,King S,Sessions RB,Kay A,Farris M,Broadbridge R,Kehoe O,Kungl AJ,Middleton J

Tue May 01 00:00:00 EDT 2018

Co-Inflammatory Roles of TGFβ1 in the Presence of TNFα Drive a Pro-inflammatory Fate in Mesenchymal Stem Cells.

Frontiers in immunology

Lerrer S,Liubomirski Y,Bott A,Abnaof K,Oren N,Yousaf A,Körner C,Meshel T,Wiemann S,Ben-Baruch A

500-P28Bt was used in Enzyme-linked immunosorbent assay to demonstrate a TNFα + TGFβ1-driven pro-inflammatory fate in MSCs, identify specific molecular mechanisms involved, and propose that TNFα + TGFβ1-stimulated MSCs influence the tumor niche.

Wed Nov 20 00:00:00 EST 2019

Regulation of the inflammatory profile of stromal cells in human breast cancer: prominent roles for TNF-α and the NF-κB pathway.

Stem cell research & therapy

Katanov C,Lerrer S,Liubomirski Y,Leider-Trejo L,Meshel T,Bar J,Feniger-Barish R,Kamer I,Soria-Artzi G,Kahani H,Banerjee D,Ben-Baruch A

500-P28Bt was used in Enzyme-linked immunosorbent assay to study the impact of tumour necrosis factor α and interleukin 1β on the inflammatory phenotype of cancer-associated fibroblasts and mesenchymal stem cells.

Fri May 01 00:00:00 EDT 2015

The Positively Charged COOH-terminal Glycosaminoglycan-binding CXCL9(74-103) Peptide Inhibits CXCL8-induced Neutrophil Extravasation and Monosodium Urate Crystal-induced Gout in Mice.

The Journal of biological chemistry

Vanheule V,Janssens R,Boff D,Kitic N,Berghmans N,Ronsse I,Kungl AJ,Amaral FA,Teixeira MM,Van Damme J,Proost P,Mortier A

Fri Aug 28 00:00:00 EDT 2015

TSG-6 inhibits neutrophil migration via direct interaction with the chemokine CXCL8.

Journal of immunology (Baltimore, Md. : 1950)

Dyer DP,Thomson JM,Hermant A,Jowitt TA,Handel TM,Proudfoot AE,Day AJ,Milner CM

Sat Mar 01 00:00:00 EST 2014

The inflammatory cytokine TNFα cooperates with Ras in elevating metastasis and turns WT-Ras to a tumor-promoting entity in MCF-7 cells.

BMC cancer

Leibovich-Rivkin T,Liubomirski Y,Meshel T,Abashidze A,Brisker D,Solomon H,Rotter V,Weil M,Ben-Baruch A

500-P28Bt was used in Enzyme-linked immunosorbent assay to determine the relative contribution of two processes to breast cancer progression: (1) Intrinsic events, such as activation of the Ras pathway and down-regulation of p53; (2) The inflammatory cytokines TNFα and IL-1β, shown in our published studies to be highly expressed in tumors of >80% of breast cancer patients with recurrent disease.

Thu Mar 06 00:00:00 EST 2014

Progression of luminal breast tumors is promoted by ménage à trois between the inflammatory cytokine TNFα and the hormonal and growth-supporting arms of the tumor microenvironment.

Mediators of inflammation

Weitzenfeld P,Meron N,Leibovich-Rivkin T,Meshel T,Ben-Baruch A

500-P28Bt was used in Enzyme-linked immunosorbent assay to indicate that TNF α can turn into a strong prometastatic factor, suggesting a paradigm shift in which clinically approved inhibitors of TNFα would be applied in breast cancer therapy.

Thu Jun 05 00:00:00 EDT 2014

Inflammatory factors of the tumor microenvironment induce plasticity in nontransformed breast epithelial cells: EMT, invasion, and collapse of normally organized breast textures.

Neoplasia (New York, N.Y.)

Leibovich-Rivkin T,Liubomirski Y,Bernstein B,Meshel T,Ben-Baruch A

500-P28Bt was used in Enzyme-linked immunosorbent assay to reveal novel pathways through which the inflammatory microenvironment may contribute to relapsed disease in breast cancer.

Sun Dec 01 00:00:00 EST 2013

B7-H3 contributes to the metastatic capacity of melanoma cells by modulation of known metastasis-associated genes.

International journal of cancer

Tekle C,Nygren MK,Chen YW,Dybsjord I,Nesland JM,Maelandsmo GM,Fodstad O

500-P28Bt was used in Enzyme-linked immunosorbent assay to investigate B7-H3 in anti tumour immunity during cancer progression.

Tue May 15 00:00:00 EDT 2012

Trans- but not cis-resveratrol impairs angiotensin-II-mediated vascular inflammation through inhibition of NF-κB activation and peroxisome proliferator-activated receptor-gamma upregulation.

Journal of immunology (Baltimore, Md. : 1950)

Rius C,Abu-Taha M,Hermenegildo C,Piqueras L,Cerda-Nicolas JM,Issekutz AC,Estañ L,Cortijo J,Morcillo EJ,Orallo F,Sanz MJ

Wed Sep 15 00:00:00 EDT 2010

Citrullination of CXCL8 by peptidylarginine deiminase alters receptor usage, prevents proteolysis, and dampens tissue inflammation.

The Journal of experimental medicine

Proost P,Loos T,Mortier A,Schutyser E,Gouwy M,Noppen S,Dillen C,Ronsse I,Conings R,Struyf S,Opdenakker G,Maudgal PC,Van Damme J

Mon Sep 01 00:00:00 EDT 2008

Characterization of a novel chemokine-containing storage granule in endothelial cells: evidence for preferential exocytosis mediated by protein kinase A and diacylglycerol.

Journal of immunology (Baltimore, Md. : 1950)

Øynebråten I,Barois N,Hagelsteen K,Johansen FE,Bakke O,Haraldsen G

Sat Oct 15 00:00:00 EDT 2005

Bioinformatics

Protein Aliases: alveolar macrophage chemotactic factor I; ANX7; beta endothelial cell-derived neutrophil activating peptide; beta-thromboglobulin-like protein; C-X-C motif chemokine 8; Chemokine (C-X-C motif) ligand 8; Emoctakin; GCP-1; Granulocyte chemotactic protein 1; IL-8; ILN; Interleukin; interleukin 8; Interleukin-8; Interleukin8; lung giant cell carcinoma-derived chemotactic protein; lymphocyte derived neutrophil activating peptide; MDNCF; MONAP; Monocyte-derived neutrophil chemotactic factor; Monocyte-derived neutrophil-activating peptide; NAP-1; neutrophil-activating peptide 1; Neutrophil-activating protein 1; Protein 3-10C; RP11-537A6.8; small inducible cytokine subfamily B, member 8; SNX; SYNEXIN; T-cell chemotactic factor; tumor necrosis factor-induced gene 1

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Gene Aliases: CXCL8; GCP-1; GCP1; IL8; LECT; LUCT; LYNAP; MDNCF; MONAP; NAF; NAP-1; NAP1

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UniProt ID: (Human) P10145

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Entrez Gene ID: (Human) 3576

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Function(s)

interleukin-8 receptor binding protein binding chemokine activity chemokine

Process(es)

angiogenesis response to molecule of bacterial origin movement of cell or subcellular component chemotaxis inflammatory response immune response cell cycle arrest signal transduction G-protein coupled receptor signaling pathway negative regulation of cell proliferation calcium-mediated signaling regulation of cell adhesion neutrophil chemotaxis receptor internalization response to endoplasmic reticulum stress intracellular signal transduction PERK-mediated unfolded protein response neutrophil activation cellular response to fibroblast growth factor stimulus regulation of single stranded viral RNA replication via double stranded DNA intermediate negative regulation of G-protein coupled receptor protein signaling pathway positive regulation of angiogenesis embryonic digestive tract development induction of positive chemotaxis chemokine-mediated signaling pathway cellular response to lipopolysaccharide cellular response to interleukin-1 cellular response to tumor necrosis factor positive regulation of neutrophil chemotaxis

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IL-8 (CXCL8) Polyclonal Antibody, Biotin, PeproTech®

IL-8 (CXCL8) Antibody (500-P28BT-25UG) in ELISA

Product Details

500-P28BT-25UG

Western Blot (WB)

ELISA (ELISA)

In vitro Assay (IV)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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