Invitrogen

IL-8 (CXCL8) Polyclonal Antibody

IL-8 (CXCL8) Antibody in Western Blot (WB)

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IL-8 (CXCL8) Antibody (AHC0881) in WB

Western blot was performed using Anti-IL-8 (CXCL8) Polyclonal Antibody(Product # AHC0881) and a 11kDa band corresponding to IL-8 (CXCL8) was observed in Human PBMC and U-87 MG with Thapsigargin and PTI treatment. Whole cell extracts (30 µg lysate) of U-87 MG (Lane 1), U-87 MG treated with PTI (1X for 4hr) (Lane 2), U-87 MG treated with Thapsigargin (1uM for 24hr) followed by PTI (1X for 4hr) (Lane 3) and Human PBMC (Lane 4) were electrophoresed using Novex™ 16% Tricine Protein Gel (Product # EC6695BOX). Resolved proteins were then transfer... View More

IL-8 (CXCL8) Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

IL-8 (CXCL8) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

IL-8 (CXCL8) Antibody in Immunohistochemistry (Frozen) (IHC (F))

IL-8 (CXCL8) Antibody in Immunohistochemistry (IHC)

IL-8 (CXCL8) Antibody in Neutralization (Neu)

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Product Details

AHC0881

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

Immunohistochemistry (IHC)

Assay-dependent

View 9 publications 9 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 5 publications 5 publications

Immunohistochemistry (Frozen) (IHC (F))

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

View 1 publication 1 publication

Neutralization (Neu)

Assay-dependent

View 1 publication 1 publication

In vitro Assay (IV)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E.coli derived Recombinant Human IL-8 (CXCL8) (72 a.a.)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Storage buffer

PBS, pH 7.2

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536263

Product Specific Information

Centrifuge vial prior to opening. Reconstitute in sterile water to 0.1-1.0 mg/mL.

Target Information

Interleukin 8 (IL-8, CXCL8) is a 72 amino acid pro-inflammatory factor which belongs to the CXC subfamily of chemokines, and are bound by the cell surface receptors IL-8RA and IL-8RB. IL-8 functions as a chemoattractant and potent angiogenic factor. The expression and secretion of IL-8 can be induced by diverse inflammatory stimuli in many cells, including macrophages and endothelial cells. In endothelial cells, IL-8 is present in storage vesicles called Weibel-Palade bodies. IL-8, first isolated from osteosarcoma cells, contains the ELR-motif (N-terminal Glu-Leu-Arg amino acid sequence) and signals through the CXCR1 and CXCR2 receptors. Previous nomenclature for IL-8 includes neutrophil activating protein 1 (NAP-1), granulocyte chemotactic protein 1 (GCP-1), monocyte-derived neutrophil-activating peptide (MONAP) and protein 3-10C. IL-8 and ten other members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q. Cancer studies have demonstrated a role for IL-8 in the angiogenesis and growth of tumours, and IL-8 is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Combined application of geranylgeranylacetone and amelogenin promotes angiogenesis and wound healing in human periodontal ligament cells.

Journal of cellular biochemistry

Yamato H,Sanui T,Yotsumoto K,Nakao Y,Watanabe Y,Hayashi C,Aihara R,Iwashita M,Tanaka U,Taketomi T,Fukuda T,Nishimura F

AHC0881 was used in ELISA, Immunohistochemistry, Neutralization, Western Blot, In Vitro Assay to suggest that the combined application of GGA and amelogenin may establish a suitable environment for periodontal wound healing and further drive the development of novel therapeutics for periodontal tissue regeneration.

Thu Jul 01 00:00:00 EDT 2021

Combined application of geranylgeranylacetone and amelogenin promotes angiogenesis and wound healing in human periodontal ligament cells.

Journal of cellular biochemistry

Yamato H,Sanui T,Yotsumoto K,Nakao Y,Watanabe Y,Hayashi C,Aihara R,Iwashita M,Tanaka U,Taketomi T,Fukuda T,Nishimura F

Thu Jul 01 00:00:00 EDT 2021

Prognostic and Pathophysiologic Significance of IL-8 (CXCL8) in Biliary Atresia.

Journal of clinical medicine

Godbole N,Nyholm I,Hukkinen M,Davidson JR,Tyraskis A,Eloranta K,Andersson N,Lohi J,Heikkilä P,Kyrönlahti A,Pihlajoki M,Davenport M,Heikinheimo M,Pakarinen MP

AHC0881 was used in Immunohistochemistry to study liver and serum IL-8 expression in a large biliary atresia (BA) cohort and explore its prognostic and pathophysiological potential.

Fri Jun 18 00:00:00 EDT 2021

Toll-Like Receptor 3 as a Recurrence Risk Factor and a Potential Molecular Therapeutic Target in Colorectal Cancer.

Clinical and experimental gastroenterology

Yoshida T,Miura T,Matsumiya T,Yoshida H,Morohashi H,Sakamoto Y,Kurose A,Imaizumi T,Hakamada K

AHC0881 was used in Immunohistochemistry to determine that TLR3 expression can be used as a risk factor for the recurrence of colorectal cancer, as well as a potential target for new therapeutic strategies.

Sun Apr 17 00:00:00 EDT 2022

Toll-Like Receptor 3 as a Recurrence Risk Factor and a Potential Molecular Therapeutic Target in Colorectal Cancer.

Clinical and experimental gastroenterology

Yoshida T,Miura T,Matsumiya T,Yoshida H,Morohashi H,Sakamoto Y,Kurose A,Imaizumi T,Hakamada K

Sun Apr 17 00:00:00 EDT 2022

Prognostic Significance of Lymphocyte Infiltration and a Stromal Immunostaining of a Bladder Cancer Associated Diagnostic Panel in Urothelial Carcinoma.

Diagnostics (Basel, Switzerland)

Furuya H,Chan OTM,Hokutan K,Tsukikawa Y,Chee K,Kozai L,Chan KS,Dai Y,Wong RS,Rosser CJ

AHC0881 was used in Immunohistochemistry to report on the presence of 10 biomarkers in the stroma of human bladder cancer and to determine the clinical significance of the signature.

Sat Dec 28 00:00:00 EST 2019

Prognostic Significance of Lymphocyte Infiltration and a Stromal Immunostaining of a Bladder Cancer Associated Diagnostic Panel in Urothelial Carcinoma.

Diagnostics (Basel, Switzerland)

Furuya H,Chan OTM,Hokutan K,Tsukikawa Y,Chee K,Kozai L,Chan KS,Dai Y,Wong RS,Rosser CJ

AHC0881 was used in Immunohistochemistry to report on the presence of 10 biomarkers in the stroma of human bladder cancer and to determine the clinical significance of the signature.

Sat Dec 28 00:00:00 EST 2019

Immunohistochemical analysis of IL-6, IL-8/CXCR2 axis, Tyr p-STAT-3, and SOCS-3 in lymph nodes from patients with chronic lymphocytic leukemia: correlation between microvascular characteristics and prognostic significance.

BioMed research international

Levidou G,Sachanas S,Pangalis GA,Kalpadakis C,Yiakoumis X,Moschogiannis M,Sepsa A,Lakiotaki E,Milionis V,Kyrtsonis MC,Vassilakopoulos TP,Tsirkinidis P,Kontopidou F,Kokoris S,Siakantaris M,Angelopoulou M,Papadaki H,Kavantzas N,Panayiotidis P,Patsouris E,Korkolopoulou P

AHC0881 was used in immunohistochemistry - paraffin section to study the correlation of microvascular characteristics and prognostic differences with immunohistochemical analysis of IL-6, IL-8/CXCR2 axis, Tyr p-STAT-3, and SOCS-3 in chronic lymphocytic le

Mon Jan 05 00:00:00 EST 2015

Validation and clinicopathologic associations of a urine-based bladder cancer biomarker signature.

Diagnostic pathology

Zhang G,Gomes-Giacoia E,Dai Y,Lawton A,Miyake M,Furuya H,Goodison S,Rosser CJ

AHC0881 was used in immunohistochemistry - paraffin section to validate the expression of a urine-based bladder cancer associated diagnostic signature

Wed Nov 12 00:00:00 EST 2014

The role of CXC-chemokine receptor CXCR2 and suppressor of cytokine signaling-3 (SOCS-3) in renal cell carcinoma.

BMC cancer

Stofas A,Levidou G,Piperi C,Adamopoulos C,Dalagiorgou G,Bamias A,Karadimou A,Lainakis GA,Papadoukakis S,Stravodimos K,Dimopoulos MA,Patsouris E,Gakiopoulou H,Korkolopoulou P

AHC0881 was used in immunohistochemistry - paraffin section and western blot to study the expression and role of CXCR2 and SOCS3 in renal cell carcinoma aggresiveness and metastasis

Tue Mar 04 00:00:00 EST 2014

Immunohistological pointers to a possible role for excessive cathelicidin (LL-37) expression by apocrine sweat glands in the pathogenesis of hidradenitis suppurativa/acne inversa.

The British journal of dermatology

Emelianov VU,Bechara FG,Gläser R,Langan EA,Taungjaruwinai WM,Schröder JM,Meyer KC,Paus R

AHC0881 was used in immunohistochemistry - frozen section to investigate the cause of follicular occlusion.

Tue May 01 00:00:00 EDT 2012

Interleukin-8, a promising predictor for prognosis of pancreatic cancer.

World journal of gastroenterology

Chen Y,Shi M,Yu GZ,Qin XR,Jin G,Chen P,Zhu MH

AHC0881 was used in immunohistochemistry - paraffin section to assess IL-8 as a biomarker for prognosis of pancreatic cancer.

Wed Mar 14 00:00:00 EDT 2012

Cytokines and growth factors expressed by human cutaneous melanoma.

Cancers

Elias EG,Hasskamp JH,Sharma BK

Published figure using IL-8 (CXCL8) polyclonal antibody (Product # AHC0881) in Immunohistochemistry

Fri May 07 00:00:00 EDT 2010

Stress effects on FosB- and interleukin-8 (IL8)-driven ovarian cancer growth and metastasis.

The Journal of biological chemistry

Shahzad MM,Arevalo JM,Armaiz-Pena GN,Lu C,Stone RL,Moreno-Smith M,Nishimura M,Lee JW,Jennings NB,Bottsford-Miller J,Vivas-Mejia P,Lutgendorf SK,Lopez-Berestein G,Bar-Eli M,Cole SW,Sood AK

Fri Nov 12 00:00:00 EST 2010

Modification of the primary tumor microenvironment by transforming growth factor alpha-epidermal growth factor receptor signaling promotes metastasis in an orthotopic colon cancer model.

The American journal of pathology

Sasaki T,Nakamura T,Rebhun RB,Cheng H,Hale KS,Tsan RZ,Fidler IJ,Langley RR

Published figure using IL-8 (CXCL8) polyclonal antibody (Product # AHC0881) in Immunohistochemistry

Tue Jul 01 00:00:00 EDT 2008

Modification of the primary tumor microenvironment by transforming growth factor alpha-epidermal growth factor receptor signaling promotes metastasis in an orthotopic colon cancer model.

The American journal of pathology

Sasaki T,Nakamura T,Rebhun RB,Cheng H,Hale KS,Tsan RZ,Fidler IJ,Langley RR

Published figure using IL-8 (CXCL8) polyclonal antibody (Product # AHC0881) in Immunohistochemistry

Tue Jul 01 00:00:00 EDT 2008

Clinical and biological significance of vascular endothelial growth factor in endometrial cancer.

Clinical cancer research : an official journal of the American Association for Cancer Research

Kamat AA,Merritt WM,Coffey D,Lin YG,Patel PR,Broaddus R,Nugent E,Han LY,Landen CN,Spannuth WA,Lu C,Coleman RL,Gershenson DM,Sood AK

Sat Dec 15 00:00:00 EST 2007

Induction of CXC chemokines in A549 airway epithelial cells by trypsin and staphylococcal proteases - a possible route for neutrophilic inflammation in chronic rhinosinusitis.

Clinical and experimental immunology

Sachse F,von Eiff C,Stoll W,Becker K,Rudack C

AHC0881 was used in immunohistochemistry - paraffin section report that Staphylococcal serine proteases induce CXC chemokines and may play a role in neutrophilic inflammation in chronic sinusitis

Thu Jun 01 00:00:00 EDT 2006

Monocyte/macrophage and T-cell infiltrates in peritoneum of patients with ovarian cancer or benign pelvic disease.

Journal of translational medicine

Wang X,Deavers M,Patenia R,Bassett RL,Mueller P,Ma Q,Wang E,Freedman RS

AHC0881 was used in immunohistochemistry - frozen section to determine the peritoneal inflammatory cell pattern to determine their concordance with selected enhanced genes in patients with epithelial ovarian cancer

Thu Jul 06 00:00:00 EDT 2006

Modulation of bone microenvironment with zoledronate enhances the therapeutic effects of STI571 and paclitaxel against experimental bone metastasis of human prostate cancer.

Cancer research

Kim SJ,Uehara H,Yazici S,He J,Langley RR,Mathew P,Fan D,Fidler IJ

Sun May 01 00:00:00 EDT 2005

Specific C-terminal cleavage and inactivation of interleukin-8 by invasive disease isolates of Streptococcus pyogenes.

The Journal of infectious diseases

Edwards RJ,Taylor GW,Ferguson M,Murray S,Rendell N,Wrigley A,Bai Z,Boyle J,Finney SJ,Jones A,Russell HH,Turner C,Cohen J,Faulkner L,Sriskandan S

Thu Sep 01 00:00:00 EDT 2005

Differential expression of interleukin-8 and its receptors in the neuroendocrine and non-neuroendocrine compartments of prostate cancer.

The American journal of pathology

Huang J,Yao JL,Zhang L,Bourne PA,Quinn AM,di Sant'Agnese PA,Reeder JE

Published figure using IL-8 (CXCL8) polyclonal antibody (Product # AHC0881) in Immunohistochemistry

Wed Jun 01 00:00:00 EDT 2005

Bioinformatics

Protein Aliases: alveolar macrophage chemotactic factor I; ANX7; beta endothelial cell-derived neutrophil activating peptide; beta-thromboglobulin-like protein; C-X-C motif chemokine 8; Chemokine (C-X-C motif) ligand 8; Emoctakin; GCP-1; Granulocyte chemotactic protein 1; IL-8; ILN; Interleukin; interleukin 8; Interleukin-8; Interleukin8; lung giant cell carcinoma-derived chemotactic protein; lymphocyte derived neutrophil activating peptide; MDNCF; MONAP; Monocyte-derived neutrophil chemotactic factor; Monocyte-derived neutrophil-activating peptide; NAP-1; neutrophil-activating peptide 1; Neutrophil-activating protein 1; Protein 3-10C; RP11-537A6.8; small inducible cytokine subfamily B, member 8; SNX; SYNEXIN; T-cell chemotactic factor; tumor necrosis factor-induced gene 1

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Gene Aliases: CXCL8; GCP-1; GCP1; IL8; LECT; LUCT; LYNAP; MDNCF; MONAP; NAF; NAP-1; NAP1

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UniProt ID: (Human) P10145

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Entrez Gene ID: (Human) 3576

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Function(s)

interleukin-8 receptor binding protein binding chemokine activity chemokine

Process(es)

angiogenesis response to molecule of bacterial origin movement of cell or subcellular component chemotaxis inflammatory response immune response cell cycle arrest signal transduction G-protein coupled receptor signaling pathway negative regulation of cell proliferation calcium-mediated signaling regulation of cell adhesion neutrophil chemotaxis receptor internalization response to endoplasmic reticulum stress intracellular signal transduction PERK-mediated unfolded protein response neutrophil activation cellular response to fibroblast growth factor stimulus regulation of single stranded viral RNA replication via double stranded DNA intermediate negative regulation of G-protein coupled receptor protein signaling pathway positive regulation of angiogenesis embryonic digestive tract development induction of positive chemotaxis chemokine-mediated signaling pathway cellular response to lipopolysaccharide cellular response to interleukin-1 cellular response to tumor necrosis factor positive regulation of neutrophil chemotaxis

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IL-8 (CXCL8) Polyclonal Antibody

IL-8 (CXCL8) Antibody (AHC0881) in WB

Product Details

AHC0881

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Neutralization (Neu)

In vitro Assay (IV)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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