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Invitrogen
IL-8 (CXCL8) Polyclonal Antibody
Advanced Verification
This Antibody was verified by Neutralization to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 3
IL-8 (CXCL8) Antibody (PA5-47166) in ICC/IF
Immunocytochemistry analysis of IL-8 (CXCL8) in immersion fixed canine peripheral blood mononuclear cells (PBMCs) treated with Concanavalin A. Samples were incubated in IL-8 (CXCL8) polyclonal antibody (Product # PA5-47166) using a dilution of 15 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specif... View More
Product Details
PA5-47166
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived recombinant canine CXCL8/IL-8 Ala23-Pro101 and and Val28-Pro101
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2608808
Product Specific Information
In Western blots, less than 1% cross-reactivity with recombinant human CXCL8, recombinant porcine CXCL8, recombinant rat (rr) CINC-1, rrCINC-2 alpha, and rrCINC-2 beta is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.
Target Information
Interleukin 8 (IL-8, CXCL8) is a 72 amino acid pro-inflammatory factor which belongs to the CXC subfamily of chemokines, and are bound by the cell surface receptors IL-8RA and IL-8RB. IL-8 functions as a chemoattractant and potent angiogenic factor. The expression and secretion of IL-8 can be induced by diverse inflammatory stimuli in many cells, including macrophages and endothelial cells. In endothelial cells, IL-8 is present in storage vesicles called Weibel-Palade bodies. IL-8, first isolated from osteosarcoma cells, contains the ELR-motif (N-terminal Glu-Leu-Arg amino acid sequence) and signals through the CXCR1 and CXCR2 receptors. Previous nomenclature for IL-8 includes neutrophil activating protein 1 (NAP-1), granulocyte chemotactic protein 1 (GCP-1), monocyte-derived neutrophil-activating peptide (MONAP) and protein 3-10C. IL-8 and ten other members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q. Cancer studies have demonstrated a role for IL-8 in the angiogenesis and growth of tumours, and IL-8 is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ANX7; C-X-C motif chemokine 8; Chemokine (C-X-C motif) ligand 8; cytokine 8; IL-8; ILN; Interleukin; Interleukin-8; Interleukin8; RP11-537A6.8; SNX; SYNEXIN
Gene Aliases: CXCL8; IL8
UniProt ID: (Dog) P41324
Entrez Gene ID: (Dog) 403850
cell cycle arrest
regulation of cell adhesion
receptor internalization
response to endoplasmic reticulum stress
intracellular signal transduction
neutrophil activation
cellular response to fibroblast growth factor stimulus
regulation of single stranded viral RNA replication via double stranded DNA intermediate
negative regulation of G-protein coupled receptor protein signaling pathway
positive regulation of angiogenesis
embryonic digestive tract development
induction of positive chemotaxis
cellular response to interleukin-1
cellular response to tumor necrosis factor
regulation of entry of bacterium into host cell
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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