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Invitrogen
IL-8 (CXCL8) Polyclonal Antibody
Advanced Verification
This Antibody was verified by Neutralization to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 3
IL-8 (CXCL8) Antibody (PA5-47289) in ICC/IF
Immunocytochemistry analysis of IL-8 (CXCL8) in immersion fixed feline peripheral blood mononuclear cells (PBMCs). Samples were incubated in IL-8 (CXCL8) polyclonal antibody (Product # PA5-47289) using a dilution of 15 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to... View More
Product Details
PA5-47289
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Cat
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived recombinant feline CXCL8/IL-8 Ala23-Ala101
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2576462
Product Specific Information
In direct ELISAs, approximately 40% cross-reactivity with recombinant canine IL-8 is observed and 15% cross-reactivity with recombinant porcine IL-8 and recombinant human IL-8 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.
Target Information
Interleukin 8 (IL-8, CXCL8) is a 72 amino acid pro-inflammatory factor which belongs to the CXC subfamily of chemokines, and are bound by the cell surface receptors IL-8RA and IL-8RB. IL-8 functions as a chemoattractant and potent angiogenic factor. The expression and secretion of IL-8 can be induced by diverse inflammatory stimuli in many cells, including macrophages and endothelial cells. In endothelial cells, IL-8 is present in storage vesicles called Weibel-Palade bodies. IL-8, first isolated from osteosarcoma cells, contains the ELR-motif (N-terminal Glu-Leu-Arg amino acid sequence) and signals through the CXCR1 and CXCR2 receptors. Previous nomenclature for IL-8 includes neutrophil activating protein 1 (NAP-1), granulocyte chemotactic protein 1 (GCP-1), monocyte-derived neutrophil-activating peptide (MONAP) and protein 3-10C. IL-8 and ten other members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q. Cancer studies have demonstrated a role for IL-8 in the angiogenesis and growth of tumours, and IL-8 is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ANX7; C-X-C motif chemokine 8; Chemokine (C-X-C motif) ligand 8; IL-8; ILN; Interleukin; Interleukin-8; Interleukin8; RP11-537A6.8; SNX; SYNEXIN
Gene Aliases: CXCL8; IL8
UniProt ID: (Cat) Q9XSX5
Entrez Gene ID: (Cat) 493836
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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