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IL-9 Antibody (500-P59BT-25UG) in ELISA
Sandwich ELISA: To detect Murine IL-9 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of IL-9 Polyclonal Antibody, Biotin (Product # 500-P59BT-1MG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech IL-9 Polyclonal Antibody (Product # 500-P59-1MG) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine IL-9.
Product Details
500-P59BT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Murine IL-9
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2930645
Product Specific Information
AA Sequence of recombinant protein: MQRCSTTWGI RDTNYLIENL KDDPPSKCSC SGNVTSCLCL SVPTDDCTTP CYREGLLQLT NATQKSRLLP VFHRVKRIVE VLKNITCPSF SCEKPCNQTM AGNTMSFLKS LLGTFQKTEM QRQKSRP.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine IL-9. Anti-Murine IL-9-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Murine IL-9 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine IL-9 (500-P59) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine IL-9.
Western Blot: To detect Murine IL-9 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Murine IL-9 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P59BT-1MG will be provided as 2 x 500 µg
Target Information
Interleukin 9 (IL-9) is produced by activated T helper 2 (Th2) lymphocytes. IL-9 regulates many cell types involved in classical Th2-associated asthma responses, including B and T lymphocytes, mast cells, eosinophils, and epithelial cells. The mouse IL-9 cDNA encodes a 144 amino acid precursor protein with an 18 amino acid signal peptide that is cleaved to form the mature cysteine-rich protein with a molecular mass of 14 kDa. Native mouse IL-9 is heavily glycosylated. Recently, TGF beta has been reported to play an important role in the differentiation of IL-17-producing T helper cells and inducible regulatory T cells by reprogramming Th2 cells to secrete IL-9, thereby driving the differentiation of Th9 cells directly.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Cytokine P40; IL-9; ILN; Interleukin; Interleukin-9; Interleukin9; T-cell growth factor P40
Gene Aliases: Il-9; Il9; P40
UniProt ID: (Mouse) P15247
Entrez Gene ID: (Mouse) 16198
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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