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IL-9 Antibody (MA5-23679) in Flow
Flow cytometry of IL-9 in Human peripheral blood mononuclear cells (PBMCs) treated with plate-bound anti-CD3 Monoclonal Antibody (5 μg/mL) and anti-CD28 Monoclonal Antibody (2 μg/mL), Recombinant Human IL-4 , and anti-Human IFN-γ Monoclonal Antibody for 5 days. Samples were incubated in (A) IL-9 monoclonal antibody (Product # MA5-23679) or (B) Mouse IgG2B Isotype Control Antibody followed by Mouse Anti-Human IFN-γ Phycoerythrin-conjugated Monoclonal Antibody. To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixati... View More
Product Details
MA5-23679
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
623153
Immunogen
Sf 21-derived recombinant human IL-9 Gln19-Ile144
Conjugate
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Purification
Protein A/G
Storage buffer
Buffered saline solution with BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2609396
Target Information
Interleukin 9 (IL-9) is produced by activated T helper 2 (Th2) lymphocytes. IL-9 regulates many cell types involved in classical Th2-associated asthma responses, including B and T lymphocytes, mast cells, eosinophils, and epithelial cells. The mouse IL-9 cDNA encodes a 144 amino acid precursor protein with an 18 amino acid signal peptide that is cleaved to form the mature cysteine-rich protein with a molecular mass of 14 kDa. Native mouse IL-9 is heavily glycosylated. Recently, TGF beta has been reported to play an important role in the differentiation of IL-17-producing T helper cells and inducible regulatory T cells by reprogramming Th2 cells to secrete IL-9, thereby driving the differentiation of Th9 cells directly.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cytokine P40; homolog of mouse T cell and mast cell growth factor 40; IL-9; ILN; Interleukin; Interleukin-9; Interleukin9; p40 cytokine; p40 T-cell and mast cell growth factor; T-cell growth factor P40
Gene Aliases: HP40; IL-9; IL9; P40
UniProt ID: (Human) P15248
Entrez Gene ID: (Human) 3578
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