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Invitrogen
IL-9 Chimeric Recombinant Rabbit Monoclonal Antibody (D9302C12)
FIGURE: 1 / 1
IL-9 Chimeric Antibody (MA5-48201) in Flow
Flow Cytometry analysis of IL-9 in Mouse splenocytes using IL-9 Chimeric Monoclonal Antibody (Product # MA5-48201). Cells were stimulated with PMA and Ionomycin in presence of Monensin for 4 hours. The cells were fixed using 2% PFA, permeabilized using 0.5% Triton and stained with anti-Fluorescein IgG antibody (isotype control, black line) or IL-9 Chimeric Monoclonal Antibody (blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AF488 antibody at a dilution of 1:1,000 and ce... View More
Product Details
MA5-48201
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG, kappa
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
D9302C12
Immunogen
This antibody was raised by immunising Armenian hamsters with Baculovirus-expressed, recombinant murine IL-9.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.02% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_3074644
Product Specific Information
Specificity: This antibody is specific for murine IL-9.
Target Information
Interleukin 9 (IL-9) is produced by activated T helper 2 (Th2) lymphocytes. IL-9 regulates many cell types involved in classical Th2-associated asthma responses, including B and T lymphocytes, mast cells, eosinophils, and epithelial cells. The mouse IL-9 cDNA encodes a 144 amino acid precursor protein with an 18 amino acid signal peptide that is cleaved to form the mature cysteine-rich protein with a molecular mass of 14 kDa. Native mouse IL-9 is heavily glycosylated. Recently, TGF beta has been reported to play an important role in the differentiation of IL-17-producing T helper cells and inducible regulatory T cells by reprogramming Th2 cells to secrete IL-9, thereby driving the differentiation of Th9 cells directly.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cytokine P40; IL-9; ILN; Interleukin; Interleukin-9; Interleukin9; T-cell growth factor P40
Gene Aliases: Il-9; Il9; P40
UniProt ID: (Mouse) P15247
Entrez Gene ID: (Mouse) 16198
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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