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IL17B Antibody (500-P248BT-25UG) in ELISA
Sandwich ELISA: To detect hIL-17B by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of IL17B Polyclonal Antibody, Biotin (Product # 500-P248BT-1MG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech IL17B Polyclonal Antibody (Product # 500-P248-1MG) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hIL-17B.
Product Details
500-P248BT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human IL-17B
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2930048
Product Specific Information
AA Sequence of recombinant protein: MQPRSPKSKR KGQGRPGPLA PGPHQVPLDL VSRMKPYARM EEYERNIEEM VAQLRNSSEL AQRKCEVNLQ LWMSNKRSLS PWGYSINHDP SRIPVDLPEA RCLCLGCVNP FTMQEDRSMV SVPVFSQVPV RRRLCPPPPR TGPCRQRAVM ETIAVGCTCI F.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IL-17B. Anti-Human IL-17B-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect hIL-17B by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human IL-17B (500-P248) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hIL-17B.
Western Blot: To detect hIL-17B by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hIL-17B is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P248BT-1MG will be provided as 2 x 500 µg
Target Information
The protein encoded by this gene is a T cell-derived cytokine that shares sequence similarity with IL17. This cytokine was reported to stimulate the release of TNF alpha (TNF) and IL1 beta (IL1B) from a monocytic cell line. Immunohistochemical analysis of several nerve tissues indicated that this cytokine is primarily localized to neuronal cell bodies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cytokine Zcyto7; cytokine-like protein ZCYTO7; IL-17B; IL-20; interleukin 20; interleukin-17 beta; Interleukin-17B; Interleukin-20; Interleukin20; neuronal interleukin-17 related factor; Neuronal interleukin-17-related factor
Gene Aliases: IL-17B; IL-20; IL17B; IL20; NIRF; UNQ516/PRO1031; ZCYTO7
UniProt ID: (Human) Q9UHF5
Entrez Gene ID: (Human) 27190
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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