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IL17B Antibody (PA5-47186) in Flow
Flow cytometry of IL17B in M‚NFS‚60 mouse myelogenous leukemia lymphoblast cell line. Samples were incubated in IL17B polyclonal antibody (Product # PA5-47186) or isotype control antibody followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody. To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Product Details
PA5-47186
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived recombinant mouse IL-17BHis21-Phe180
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2576811
Product Specific Information
In sandwich ELISAs, approximately 40% cross-reactivity with recombinant human IL-17B is observed and less than 0.3% cross-reactivity with recombinant mouse (rm) IL-17, rmIL-17C, rmIL-17D, rmIL-17E, and rmIL-17F is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.
Target Information
The protein encoded by this gene is a T cell-derived cytokine that shares sequence similarity with IL17. This cytokine was reported to stimulate the release of TNF alpha (TNF) and IL1 beta (IL1B) from a monocytic cell line. Immunohistochemical analysis of several nerve tissues indicated that this cytokine is primarily localized to neuronal cell bodies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cytokine CX1; Cytokine-like protein ZCYTO7; gene for cytokine-like protein; IL-17B; Interleukin-17B; Interleukin20; Neuronal interleukin-17-related factor
Gene Aliases: 1110006O16Rik; 1700006N07Rik; Il17b; Nirf; Zcyto7
UniProt ID: (Mouse) Q9QXT6
Entrez Gene ID: (Mouse) 56069
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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