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IL1F6 Antibody (PA5-118006) in WB
Western Blot using IL1F6 Polyclonal Antibody (Product # PA5-118006) at 1:500 dilution. Lane A: MDA-MB231 Whole Cell Lysate, Lane B: HT1080 Whole Cell Lysate, Lane C: MCF7 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 18 kDa. Observed band size: 24 kDa.
Product Details
PA5-118006
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Human IL-1F6 Protein
Conjugate
Form
Liquid
Purification
Antigen affinity chromatography, Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2902611
Target Information
The IL-1 family is comprised of 11 structurally related ligands, including recently re-named IL-36alpha (IL-1F6), beta (IL-1F8), and gamma (IL-1F9). IL-36alpha is highly expressed in psoriatic plaques, as are IL-36beta and IL-36gamma, and is the only IL-1 family member found to be expressed by T cells, as well as monocytes and B cells. Primarily found in skin and lymphoid tissues, IL-36alpha is also found to a lesser extent in tissues of the fetal brain, trachea, stomach, and intestine. IL-36alpha signals through a combination of the IL-1Rrp2 (IL-1R6) receptor, which is primarily expressed on certain dendritic cells, and the widely expressed IL-RAcP (IL-1 receptor accessory protein). IL-36alpha also functions as an agonist of NF-kappaB and MAPK pathways, and induces the production of proinflammatory cytokines and chemokines, including IL-6, IL-8, BD-2, and BD-3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FIL1 epsilon; IL-1 epsilon; IL-1F6; IL-1F6 (FIL-1-epsilon); interleukin 1; interleukin 1 family, member 6 (epsilon); interleukin 1, epsilon; Interleukin-1 epsilon; Interleukin-1 family member 6; Interleukin-36; Interleukin-36 alpha
Gene Aliases: FIL1; FIL1(EPSILON); FIL1E; IL-1F6; IL1(EPSILON); IL1E; IL1F6; IL36A
UniProt ID: (Human) Q9UHA7
Entrez Gene ID: (Human) 27179
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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