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IL1RA Antibody (PA5-95456) in WB
Western blot analysis of IL1RA in, Lane 1: rat thymus tissue lysates, Lane 2: mouse RAW246. 7 whole cell lysates, . Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 µg of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. The membrane was blocked with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with IL1RA Polyclonal A... View More
Product Details
PA5-95456
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived mouse IL1RA recombinant protein (Position: R27-Q178)
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2807259
Product Specific Information
Mouse IL1RA shares 77% and 90.7% amino acid (aa) sequence identity with human and rat IL1RA, respectively.
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
The protein encoded by this gene is a cytokine receptor that belongs to the interleukin 1 receptor family. This protein is a receptor for interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I(IL1R1/IL1RA). It is an important mediator involved in many cytokine induced immune and inflammatory responses. This gene along with interleukin 1 receptor, type II (IL1R2), interleukin 1 receptor-like 2 (IL1RL2), and interleukin 1 receptor-like 1 (IL1RL1) form a cytokine receptor gene cluster in a region mapped to chromosome 2q12.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD121A; D2S1473; IL 1 receptor antagonist; il-1 receptor; il-1r; IL-1RN; IL1 inhibitor; il1 receptor; IL1 receptor antagonist; IL1-RA; IL1R; IL1RN (IL1F3); interleukin-1 receptor; Interleukin-1 receptor antagonist protein; IRAP; MGC10430; P80
Gene Aliases: F630041P17Rik; IL-1ra; Il1rn
UniProt ID: (Mouse) P25085
Entrez Gene ID: (Mouse) 16181
fever generation
negative regulation of cytokine-mediated signaling pathway
lipid metabolic process
acute-phase response
memory
negative regulation of glutamate secretion
cytokine-mediated signaling pathway
sensory perception of pain
insulin secretion
negative regulation of cell migration
negative regulation of heterotypic cell-cell adhesion
negative regulation of apoptotic process
positive regulation of JUN kinase activity
negative regulation of membrane potential
response to glucocorticoid
extracellular negative regulation of signal transduction
negative regulation of receptor activity
negative regulation of interleukin-1-mediated signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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