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IL1RA Antibody (PA5-95457) in WB
Western blot analysis of IL1RA in Lane 1: rat testis tissue lysate. Electrophoresis was performed with 5-20% SDS-PAGE gel (70V, Stacking gel; 90V Resolving gel, Time: 2-3 hours), transferred to a nitrocellulose membrane and blocked using 5% Non-fat Milk/TBS (1.5 hrs at room temperature). Samples were incubated with IL1RA polyclonal antibody (Product # PA5-95457) using a 0.5 µg/mL dilution, followed by a goat anti-rabbit IgG-HRP at a dilution of 1:10,000, and developed with enhanced chemiluminescence (ECL).
Product Details
PA5-95457
Product Specifications
Species Reactivity
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived rat IL1RA recombinant protein (Position: H27-Q178).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2807260
Product Specific Information
Rat IL1RA shares 77.6% and 90.7% amino acid (aa) sequence identity with human and mouse IL1RA, respectively.
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
The protein encoded by this gene is a cytokine receptor that belongs to the interleukin 1 receptor family. This protein is a receptor for interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I(IL1R1/IL1RA). It is an important mediator involved in many cytokine induced immune and inflammatory responses. This gene along with interleukin 1 receptor, type II (IL1R2), interleukin 1 receptor-like 2 (IL1RL2), and interleukin 1 receptor-like 1 (IL1RL1) form a cytokine receptor gene cluster in a region mapped to chromosome 2q12.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD121A; D2S1473; IL 1 receptor antagonist; il-1 receptor; il-1r; IL-1RN; IL1 inhibitor; il1 receptor; IL1 receptor antagonist; IL1-RA; IL1R; IL1RN (IL1F3); interleukin-1 receptor; Interleukin-1 receptor antagonist protein; IRAP; MGC10430; P80
Gene Aliases: IL-1ra; Il1rn
UniProt ID: (Rat) P25086
Entrez Gene ID: (Rat) 60582
fever generation
negative regulation of cytokine-mediated signaling pathway
chronic inflammatory response to antigenic stimulus
lipid metabolic process
acute-phase response
female pregnancy
memory
negative regulation of signal transduction
response to organonitrogen compound
negative regulation of glutamate secretion
response to organic cyclic compound
response to activity
cytokine-mediated signaling pathway
sensory perception of pain
carboxylic acid metabolic process
insulin secretion
negative regulation of cell migration
response to lipopolysaccharide
negative regulation of heterotypic cell-cell adhesion
response to drug
response to starvation
negative regulation of apoptotic process
positive regulation of JUN kinase activity
negative regulation of membrane potential
response to glucocorticoid
response to interleukin-1
response to interleukin-4
cellular response to norepinephrine stimulus
negative regulation of interleukin-1-mediated signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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