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Invitrogen
IL1RA Monoclonal Antibody (02)
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 6
IL1RA Antibody (MA5-29352) in ICC/IF
Immunofluorescence staining of Human IL1Ra in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with IL1RA Monoclonal Antibody (2) (Product # MA5-29352, 1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green). Positive staining was localized to cytoplasm.
Product Details
MA5-29352
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
02
Immunogen
Recombinant Human IL-1RA protein (Arg26-Glu177)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2785232
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
This antibody has specificity for Human IL-1RA.
Target Information
The protein encoded by this gene is a cytokine receptor that belongs to the interleukin 1 receptor family. This protein is a receptor for interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I(IL1R1/IL1RA). It is an important mediator involved in many cytokine induced immune and inflammatory responses. This gene along with interleukin 1 receptor, type II (IL1R2), interleukin 1 receptor-like 2 (IL1RL2), and interleukin 1 receptor-like 1 (IL1RL1) form a cytokine receptor gene cluster in a region mapped to chromosome 2q12.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD121A; D2S1473; ICIL-1RA; IL 1 receptor antagonist; il-1 receptor; il-1r; IL-1RN; IL1 inhibitor; il1 receptor; IL1 receptor antagonist; IL1-RA; IL1R; IL1RN (IL1F3); interleukin-1 receptor; Interleukin-1 receptor antagonist protein; intracellular IL-1 receptor antagonist type II; intracellular interleukin-1 receptor antagonist (icIL-1ra); IRAP; MGC10430; P80; type II interleukin-1 receptor antagonist
Gene Aliases: DIRA; F630041P17Rik; ICIL-1RA; IL-1ra; IL-1ra3; IL-1RN; IL1F3; IL1RA; IL1RN; IRAP; MVCD4
UniProt ID: (Human) P18510, (Mouse) P25085, (Rat) P25086
Entrez Gene ID: (Human) 3557, (Mouse) 16181, (Rat) 60582
cytokine activity
interleukin-1, Type I receptor binding
interleukin-1, Type II receptor binding
interleukin-1 receptor antagonist activity
protein binding
interleukin-1 Type I receptor antagonist activity
interleukin-1 Type II receptor antagonist activity
interleukin-1 receptor binding
interleukin superfamily
lipid metabolic process
immune response
insulin secretion
negative regulation of heterotypic cell-cell adhesion
response to glucocorticoid
negative regulation of interleukin-1-mediated signaling pathway
fever generation
negative regulation of cytokine-mediated signaling pathway
acute-phase response
memory
negative regulation of glutamate secretion
cytokine-mediated signaling pathway
sensory perception of pain
negative regulation of cell migration
negative regulation of apoptotic process
positive regulation of JUN kinase activity
negative regulation of membrane potential
extracellular negative regulation of signal transduction
negative regulation of receptor activity
chronic inflammatory response to antigenic stimulus
female pregnancy
negative regulation of signal transduction
response to organonitrogen compound
response to organic cyclic compound
response to activity
carboxylic acid metabolic process
response to lipopolysaccharide
response to drug
response to starvation
response to interleukin-1
response to interleukin-4
cellular response to norepinephrine stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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