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IL4R (soluble) Antibody (500-P325-1MG) in WB
Western Blot: To detect Human sIL-4 Receptor alpha by Western Blot analysis IL4R (soluble) Polyclonal Antibody (Product # 500-P325-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human sIL-4 Receptor alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P325-1MG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
CHO cells-derived Recombinant Human IL-4 Receptor alpha
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2930423
Product Specific Information
AA Sequence of recombinant protein: GNMKVLQEPT CVSDYMSIST CEWKMNGPTN CSTELRLLYQ LVFLLSEAHT CIPENNGGAG CVCHLLMDDV VSADNYTLDL WAGQQLLWKG SFKPSEHVKP RAPGNLTVHT NVSDTLLLTW SNPYPPDNYL YNHLTYAVNI WSENDPADFR IYNVTYLEPS LRIAASTLKS GISYRARVRA WAQCYNTTWS EWSPSTKWHN SYREPFEQH.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human sIL-4 Receptor alpha. Anti-Human sIL-4 Receptor alpha-specific antibody was purified by affinity chromatography employing an immobilized Human sIL-4 Receptor alpha matrix.
Sandwich ELISA: To detect Human sIL-4 Receptor alpha by sandwich ELISA (using 100 µL/well), a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human sIL-4 Receptor alpha (500-P325BT) as a detection antibody, allows the detection of at least 2000-4000 pg/mL of Recombinant Human sIL-4 Receptor alpha.
Western Blot: To detect Human sIL-4 Receptor alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human sIL-4 Receptor alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
IL4 Receptor alpha (IL4RA) is the alpha chain of the interleukin 4 receptor which binds to both interleukin 4 and interleukin 13 to regulate IgE production, chemokine and mucus production at sites of allergic inflammmation. The IL4 response is involved in promoting Th2 differentiation. The secreted extracellular domain of IL4R alpha, called sIL4R alpha, can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells. It has no signaling abilities. Allelic variations in the IL4RA gene have been associated with atopy, a condition that can manifest itself as allergic rhinitis, sinusitus, asthma, or eczema.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD124; il-4 receptor; IL-4 receptor subunit alpha; IL4R nirs variant 1; interleukin-4 receptor alpha chain; Interleukin-4 receptor subunit alpha
Gene Aliases: 582J2.1; CD124; IL-4RA; IL4R; IL4RA
UniProt ID: (Human) P24394
Entrez Gene ID: (Human) 3566
production of molecular mediator involved in inflammatory response
positive regulation of immunoglobulin production
immune response
signal transduction
immunoglobulin mediated immune response
ovulation
interleukin-4-mediated signaling pathway
regulation of cell proliferation
defense response to protozoan
positive regulation of macrophage activation
positive regulation of mast cell degranulation
response to estrogen
negative regulation of T-helper 1 cell differentiation
positive regulation of T-helper 2 cell differentiation
positive regulation of chemokine secretion
positive regulation of myoblast fusion
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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