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INSR alpha Antibody (AHR0231) in ICC/IF
Immunofluorescent analysis of Insulin Receptor alpha (green) showing staining in the cytoplasm and membrane of MCF-7 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with an Insulin Receptor alpha monoclonal anti... View More
Product Details
AHR0231
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Sheep,
Pig,
Rabbit
Published species
Human,
Mouse,
Yeast
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
83-7
Immunogen
IM-9 lymphocytes followed by purified insulin receptor.
Conjugate
Form
Liquid
Concentration
0.2 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2536350
Product Specific Information
This antibody is specific for IR and shows no cross-reactivity with insulin-like growth factor (IGF)-receptors. The epitope for this monoclonal antibody is conformational and is located in exon 3.
Staining of formalin-fixed, paraffin tissues requires digestion of tissue sections with pepsin at 1mg/mL in Tris-HCl, pH 2.0, for 15 min at room temperature or 10 min at 37ºC. Recommended positive controls include IM-9 lymphocytes, placenta, or breast carcinoma.
Target Information
Biological actions of insulin and IGF-1 are mediated by their respective cell surface receptor tyrosine kinases that regulate multiple signaling pathways through activation of a series of phosphorylation cascades. The insulin receptor. Insulin/IGF-1 binding to the extracellular domain leads to autophosphorylation of downstream target proteins. These two receptors differ in sequence in regions that confer specificity for the designated ligand as well as in certain intracellular signaling domains, resulting in significant differences in the functional consequences of activation of each receptor. Defects in IR are the cause of various insulin resistance syndromes and IGF-1R defects may cause some forms of growth retardation. Both these signaling cascades are also important for the development of cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD220; CD220 beta; INSR; Insulin receptor; IR; IR alpha; IR beta
Gene Aliases: CD220; DAF-2; HHF5; INSR; ir; ir-A
UniProt ID: (Human) P06213
Entrez Gene ID: (Bovine) 408017, (Human) 3643, (Sheep) 443431, (Rabbit) 100008911, (Pig) 396755
protein tyrosine kinase activity
receptor signaling protein tyrosine kinase activity
insulin-activated receptor activity
insulin-like growth factor receptor binding
protein binding
ATP binding
GTP binding
protein phosphatase binding
lipoic acid binding
insulin-like growth factor I binding
insulin-like growth factor II binding
protein complex binding
3-phosphoinositide-dependent protein kinase binding
phosphatidylinositol 3-kinase binding
insulin binding
insulin receptor substrate binding
PTB domain binding
transmembrane signal receptor
activation of MAPK activity
negative regulation of protein phosphorylation
positive regulation of protein phosphorylation
heart morphogenesis
carbohydrate metabolic process
regulation of transcription, DNA-templated
G-protein coupled receptor signaling pathway
positive regulation of cell proliferation
insulin receptor signaling pathway
epidermis development
male gonad development
response to manganese ion
regulation of hydrogen peroxide metabolic process
positive regulation of glycoprotein biosynthetic process
negative regulation of gene expression
response to activity
peptidyl-tyrosine phosphorylation
transformation of host cell by virus
signal transduction by protein phosphorylation
male sex determination
adrenal gland development
positive regulation of cell migration
exocrine pancreas development
activation of protein kinase activity
activation of protein kinase B activity
response to estradiol
negative regulation of transporter activity
cellular response to insulin stimulus
response to vitamin D
response to testosterone
response to tumor necrosis factor
peptidyl-tyrosine autophosphorylation
glucose homeostasis
positive regulation of MAPK cascade
positive regulation of nitric oxide biosynthetic process
fat cell differentiation
response to ethanol
positive regulation of glycogen biosynthetic process
positive regulation of DNA replication
positive regulation of glycolytic process
positive regulation of mitotic nuclear division
positive regulation of transcription, DNA-templated
regulation of embryonic development
positive regulation of glucose import
protein autophosphorylation
positive regulation of developmental growth
protein heterotetramerization
response to glucocorticoid
positive regulation of meiotic cell cycle
positive regulation of protein kinase B signaling
positive regulation of respiratory burst
cellular response to growth factor stimulus
regulation of female gonad development
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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