Invitrogen

INSR beta Monoclonal Antibody (CT-3)

Advanced Verification

IP-mass spectrometry

6 Published Figures

10 References

View all (14) INSR beta antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

INSR beta Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 14

INSR beta Antibody (AHR0271) in ICC/IF

Immunofluorescent analysis of Insulin Receptor beta (green) showing staining in the cytoplasm of MCF-7 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with an Insulin Receptor beta monoclonal antibody (Product #... View More

INSR beta Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

AHR0271

Applications

Tested Dilution

Publications

Western Blot (WB)

1:25-1:50

View 5 publications 5 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

Assay-Dependent

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:10-1:100

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

CT-3

Immunogen

Recombinant fragment including the C-terminal 100 amino acid residues of human insulin receptor.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536351

Product Specific Information

Staining of formalin-fixed paraffin embedded tissue sections requires boiling the sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at room temperature for 20 minutes.

Target Information

Biological actions of insulin and IGF-1 are mediated by their respective cell surface receptor tyrosine kinases that regulate multiple signaling pathways through activation of a series of phosphorylation cascades. The insulin receptor. Insulin/IGF-1 binding to the extracellular domain leads to autophosphorylation of downstream target proteins. These two receptors differ in sequence in regions that confer specificity for the designated ligand as well as in certain intracellular signaling domains, resulting in significant differences in the functional consequences of activation of each receptor. Defects in IR are the cause of various insulin resistance syndromes and IGF-1R defects may cause some forms of growth retardation. Both these signaling cascades are also important for the development of cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Modulation of olfactory-driven behavior by metabolic signals: role of the piriform cortex.

Brain structure & function

Al Koborssy D,Palouzier-Paulignan B,Canova V,Thevenet M,Fadool DA,Julliard AK

AHR0271 was used in Immunocytochemistry-immunoflourescence to investigate if metabolic signaling pathways that are present in the anterior piriform cortex are capable of neuronal modulation and changing complex olfactory behaviors in higher olfactory centers.

Tue Jan 01 00:00:00 EST 2019

Suppression of insulin feedback enhances the efficacy of PI3K inhibitors.

Nature

Hopkins BD,Pauli C,Du X,Wang DG,Li X,Wu D,Amadiume SC,Goncalves MD,Hodakoski C,Lundquist MR,Bareja R,Ma Y,Harris EM,Sboner A,Beltran H,Rubin MA,Mukherjee S,Cantley LC

AHR0271 was used in Immunohistochemistry to study whether insulin feedback induced by phosphatidylinositol-3 kinase inhibitors may reactivate the phosphatidylinositol-3 kinase-mTOR signalling axis in tumours, thereby compromising treatment effectiveness.

Wed Aug 01 00:00:00 EDT 2018

High expression of insulin receptor on tumour-associated blood vessels in invasive bladder cancer predicts poor overall and progression-free survival.

The Journal of pathology

Roudnicky F,Dieterich LC,Poyet C,Buser L,Wild P,Tang D,Camenzind P,Ho CH,Otto VI,Detmar M

AHR0271 was used in immunohistochemistry - paraffin section to find novel biomarkers associated with bladder cancer progression

Thu Jun 01 00:00:00 EDT 2017

A persistent increase in insulin-stimulated glucose uptake by both fast-twitch and slow-twitch skeletal muscles after a single exercise session by old rats.

Age (Dordrecht, Netherlands)

Xiao Y,Sharma N,Arias EB,Castorena CM,Cartee GD

AHR0271 was used in western blot to measure the effects of exercise on glucose uptake by type I (slow-twitch) muscle from old rats.

Sat Jun 01 00:00:00 EDT 2013

Calorie restriction enhances insulin-stimulated glucose uptake and Akt phosphorylation in both fast-twitch and slow-twitch skeletal muscle of 24-month-old rats.

The journals of gerontology. Series A, Biological sciences and medical sciences

Sequea DA,Sharma N,Arias EB,Cartee GD

AHR0271 was used in western blot to examine the insulin signaling pathway in isolated epitrochlearis and soleus muscles of old rats.

Sat Dec 01 00:00:00 EST 2012

Preventing the calorie restriction-induced increase in insulin-stimulated Akt2 phosphorylation eliminates calorie restriction's effect on glucose uptake in skeletal muscle.

Biochimica et biophysica acta

Sharma N,Arias EB,Sequea DA,Cartee GD

AHR0271 was used in western blot to examine the effect of Akt2 phosphorylation on on glucose uptake in skeletal muscle

Thu Nov 01 00:00:00 EDT 2012

A physiological increase of insulin in the olfactory bulb decreases detection of a learned aversive odor and abolishes food odor-induced sniffing behavior in rats.

PloS one

Aimé P,Hegoburu C,Jaillard T,Degletagne C,Garcia S,Messaoudi B,Thevenet M,Lorsignol A,Duchamp C,Mouly AM,Julliard AK

AHR0271 was used in immunohistochemistry - frozen section to study the role of insulin in olfactory function.

Tue Jun 11 00:00:00 EDT 2013

Mechanisms for increased insulin-stimulated Akt phosphorylation and glucose uptake in fast- and slow-twitch skeletal muscles of calorie-restricted rats.

American journal of physiology. Endocrinology and metabolism

Sharma N,Arias EB,Bhat AD,Sequea DA,Ho S,Croff KK,Sajan MP,Farese RV,Cartee GD

AHR0271 was used in Western Blot to study the mechanisms behind calorie restriction, improved insulin-stimulated glucose uptake and Akt phosphorylation in skeletal muscle.

Wed Jun 01 00:00:00 EDT 2011

Generation of intracellular domain of insulin receptor tyrosine kinase by gamma-secretase.

Biochemical and biophysical research communications

Kasuga K,Kaneko H,Nishizawa M,Onodera O,Ikeuchi T

AHR0271 was used in western blot to test if the insulin receptor undergoes presenilin/gamma-secretase-dependent processing

Fri Aug 17 00:00:00 EDT 2007

In vivo antitumor activity of NVP-AEW541-A novel, potent, and selective inhibitor of the IGF-IR kinase.

Cancer cell

García-Echeverría C,Pearson MA,Marti A,Meyer T,Mestan J,Zimmermann J,Gao J,Brueggen J,Capraro HG,Cozens R,Evans DB,Fabbro D,Furet P,Porta DG,Liebetanz J,Martiny-Baron G,Ruetz S,Hofmann F

AHR0271 was used in ELISA to study the in vivo antitumor activity of a potent and selective IGF-1 receptor kinase inhibitor

Mon Mar 01 00:00:00 EST 2004

Bioinformatics

Protein Aliases: CD220; CD220 beta; Insulin receptor; IR; IR beta

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Gene Aliases: 4932439J01Rik; CD220; D630014A15Rik; HHF5; INSR; IR; IR-A; IR-B

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UniProt ID: (Human) P06213, (Mouse) P15208

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Entrez Gene ID: (Human) 3643, (Mouse) 16337

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Function(s)

protein tyrosine kinase activity receptor signaling protein tyrosine kinase activity insulin-activated receptor activity insulin-like growth factor receptor binding protein binding ATP binding GTP binding protein phosphatase binding lipoic acid binding insulin-like growth factor I binding insulin-like growth factor II binding protein complex binding 3-phosphoinositide-dependent protein kinase binding phosphatidylinositol 3-kinase binding insulin binding insulin receptor substrate binding PTB domain binding nucleotide binding protein kinase activity transmembrane receptor protein tyrosine kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups protein kinase binding protein domain specific binding transmembrane signal receptor

Process(es)

Performance Guarantee

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INSR beta Monoclonal Antibody (CT-3)

INSR beta Antibody (AHR0271) in ICC/IF

Product Details

AHR0271

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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