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IRE1 alpha Antibody (PA5-80598) in WB
Western Blot using IRE1 alpha Polyclonal Antibody (Product # PA5-80598) at 1:500 dilution. Lane A: Raji Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti- RabbitIgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 110 kDa. Observed band size: 110 kDa. (We are unsure of the identity of these extra bands).
Product Details
PA5-80598
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Human ERN1/IRE1 Protein (Pro465-Leu977)
Conjugate
Form
Liquid
Purification
Protein A, Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2787896
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
This antibody has specificity for Human ERN1/IRE1.
Target Information
ERN1 serine/threonine kinase or endoplasmic reticulum to nucleus signaling 1 is the ER to nucleus signaling 1 protein, a human homologue of the yeast Ire1 gene product which possesses intrinsic kinase activity and an endoribonuclease activity and it is important in endoplasmic reticulum-based stress signals. ERN1 controls IRE1 proteolysis in mammalian cells. ERN1 has a critical function in extraembryonic cells that is essential for fetal viability. Specifically, IRE1 acts as the sensor of unfolded proteins in the ER that initiates transmittance of the unfolded protein signal from the ER to the nucleus by splicing XBP1 mRNA converting it into a potent unfolded-protein response transcriptional activator. Null mutants are viable, but IRE1 is essential for viability under stress conditions that cause unfolded proteins to accumulate in the ER. Depletion of IRE1p through the expression of a dominant negative form of IRE1p has no effect on transfected cells, but cell death via apoptosis occurs under stress conditions that cause unfolded proteins to accumulate in the ER.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Endoplasmic reticulum-to-nucleus signaling 1; ER to nucleus signalling 1; ERN1; FLJ30999; hIRE1p; inositol-requiring 1; inositol-requiring enzyme 1; Inositol-requiring protein 1; Ire1-alpha; IRE1a; IRE1a antibody; IRE1p antibody; MGC163277; MGC163279; protein kinase/endoribonuclease; Serine/threonine-protein kinase/endoribonuclease IRE1
Gene Aliases: ERN1; hIRE1p; IRE1; IRE1a; IRE1P
UniProt ID: (Human) Q59EE2
Entrez Gene ID: (Human) 2081
endothelial cell proliferation
transcription, DNA-templated
regulation of transcription, DNA-templated
mRNA cleavage
mRNA catabolic process
protein phosphorylation
activation of signaling protein activity involved in unfolded protein response
cell cycle arrest
activation of JUN kinase activity
regulation of macroautophagy
positive regulation of RNA splicing
response to endoplasmic reticulum stress
cellular response to vascular endothelial growth factor stimulus
peptidyl-serine autophosphorylation
IRE1-mediated unfolded protein response
protein autophosphorylation
mRNA splicing, via endonucleolytic cleavage and ligation
mRNA endonucleolytic cleavage involved in unfolded protein response
intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
cellular response to glucose stimulus
RNA phosphodiester bond hydrolysis, endonucleolytic
positive regulation of endoplasmic reticulum unfolded protein response
insulin metabolic process
peptidyl-serine trans-autophosphorylation
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