Proteintech

ISG15 Polyclonal Antibody

ISG15 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 10

ISG15 Antibody (15981-1-AP) in ICC/IF

Immunofluorescent analysis of ( -20°C Ethanol ) fixed HeLa cells using 15981-1-AP (ISG15 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

ISG15 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ISG15 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

ISG15 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

15981-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 38 publications 38 publications

Immunohistochemistry (IHC)

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:50

Immunocytochemistry (ICC/IF)

1:50-1:500

View 4 publications 4 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Goat, Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

ISG15 Fusion Protein Ag8782 (1-165 aa encoded by BC009507)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.21 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MGWDLTVKM LAGNEFQVSL SSSMSVSELK AQITQKIGVH AFQQRLAVHP SGVALQDRVP LASQGLGPGS TVLLVVDKCD EPLNILVRNN KGRSSTYEVR LTQTVAHLKQ QVSGLEGVQD DLFWLTFEGK PLEDQLPLGE YGLKPLSTVF MNLRLRGGGT EPGGRS (1-165 aa encoded by B C009507)

Target Information

Ubiquitin-like protein ISG15 plays a key role in the innate immune response to viral infection either via its conjugation to a target protein (ISGylation) or via its action as a free or unconjugated protein. It is secreted from monocytes in response to type I interferons and causes natural killer (NK)-cell proliferation and an augmentation of non-MCH (major histocompatibility complex)-restricted cytotoxicity. Synthesis is stimulated by IFN-alpha or IFN-beta or IFN-omega, but not IFN-gamma. ISG15 expression is also induced by overexpression of interferon regulatory factors that participate in transcriptional regulation of IFN genes, and by influenza B virus. ISG15 is secreted by cell lines of monocyte, T-lymphocyte, B-lymphocyte, human fibroblasts, and epithelial origins. Enhancement of NK cell proliferation, augmentation of non-major histocompatibility complex-restricted cytotoxicity, and induction of IFN-gamma from T cells identify ISG15 as a member of the cytokine cascade and suggest that it may be responsible for amplifying and directing some of the immunomodulatory effects of IFN-alpha or IFN-beta. ISG15 has has also been shown to function intracellularly as a ubiquitin homolog. Mutations affecting the gene can result in Immunodeficiency 38, with basal ganglia calcification (IMD38).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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USP18 promotes endometrial receptivity via the JAK/STAT1 and the ISGylation pathway.

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Identification of ISG15 and ZFP36 as novel hypoxia- and immune-related gene signatures contributing to a new perspective for the treatment of prostate cancer by bioinformatics and experimental verification.

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ISG15 promotes esophageal squamous cell carcinoma tumorigenesis via c-MET/Fyn/β-catenin signaling pathway.

Experimental cell research

Yuan H,Zhou W,Yang Y,Xue L,Liu L,Song Y

Fri Jun 01 00:00:00 EDT 2018

ISG15 promotes esophageal squamous cell carcinoma tumorigenesis via c-MET/Fyn/β-catenin signaling pathway.

Experimental cell research

Yuan H,Zhou W,Yang Y,Xue L,Liu L,Song Y

Fri Jun 01 00:00:00 EDT 2018

ISG15 promotes esophageal squamous cell carcinoma tumorigenesis via c-MET/Fyn/β-catenin signaling pathway.

Experimental cell research

Yuan H,Zhou W,Yang Y,Xue L,Liu L,Song Y

Fri Jun 01 00:00:00 EDT 2018

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Mon Aug 12 00:00:00 EDT 2019

The S Genome Segment Is Sufficient to Maintain Pathogenicity in Intra-Clade Lassa Virus Reassortants in a Guinea Pig Model.

Frontiers in cellular and infection microbiology

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Journal of virology

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Restriction of Human Cytomegalovirus Replication by ISG15, a Host Effector Regulated by cGAS-STING Double-Stranded-DNA Sensing.

Journal of virology

Bianco C,Mohr I

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Journal of immunology (Baltimore, Md. : 1950)

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Mon Aug 15 00:00:00 EDT 2016

Network Analysis Identifies Proinflammatory Plasma Cell Polarization for Secretion of ISG15 in Human Autoimmunity.

Journal of immunology (Baltimore, Md. : 1950)

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Infection of goats with goatpox virus triggers host antiviral defense through activation of innate immune signaling.

Research in veterinary science

Zeng X,Wang S,Chi X,Chen SL,Huang S,Lin Q,Xie B,Chen JL

Mon Feb 01 00:00:00 EST 2016

Infection of goats with goatpox virus triggers host antiviral defense through activation of innate immune signaling.

Research in veterinary science

Zeng X,Wang S,Chi X,Chen SL,Huang S,Lin Q,Xie B,Chen JL

Mon Feb 01 00:00:00 EST 2016

ISGylation controls exosome secretion by promoting lysosomal degradation of MVB proteins.

Nature communications

Villarroya-Beltri C,Baixauli F,Mittelbrunn M,Fernández-Delgado I,Torralba D,Moreno-Gonzalo O,Baldanta S,Enrich C,Guerra S,Sánchez-Madrid F

Thu Nov 24 00:00:00 EST 2016

ISGylation controls exosome secretion by promoting lysosomal degradation of MVB proteins.

Nature communications

Villarroya-Beltri C,Baixauli F,Mittelbrunn M,Fernández-Delgado I,Torralba D,Moreno-Gonzalo O,Baldanta S,Enrich C,Guerra S,Sánchez-Madrid F

Thu Nov 24 00:00:00 EST 2016

ISG15 is a critical microenvironmental factor for pancreatic cancer stem cells.

Cancer research