Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 1
ISG20 Antibody (PA5-120291) in WB
Western blot analysis of ISG20 in extracts of various cell lines. Samples were incubated with ISG20 Polyclonal antibody (Product # PA5-120291) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
PA5-120291
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-70 of human ISG20 (NP_002192.2).
Conjugate
Form
Liquid
Concentration
0.91 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2913863
Product Specific Information
Positive test controls include: Raji, A-549, MCF7, Mouse spleen, Rat spleen. The target is usually found in the following locations: Cajal body, Cytoplasm, Nucleus, nucleolus.
Immunogen sequence: MAGSREVVAM DCEMVGLGPH RESGLARCSL VNVHGAVLYD KFIRPEGEIT DYRTRVSGVT PQHMVGATPF
Target Information
Interferon-induced antiviral exoribonuclease that acts on single-stranded RNA and also has minor activity towards single-stranded DNA. Exhibits antiviral activity against RNA viruses including hepatitis C virus (HCV), hepatitis A virus (HAV) and yellow fever virus (YFV) in an exonuclease-dependent manner. May also play additional roles in the maturation of snRNAs and rRNAs, and in ribosome biogenesis. [UniProt]
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: Estrogen-regulated transcript 45 protein; interferon stimulated exonuclease; interferon stimulated exonuclease 20; interferon stimulated exonuclease gene 20kDa; interferon stimulated gene 20kDa; Interferon-stimulated gene 20 kDa protein; interferon-stimulated protein; Promyelocytic leukemia nuclear body-associated protein ISG20; Protein DnaQL
Gene Aliases: 1600023I01Rik; 2010107M23Rik; 20kDa; CD25; DnaQL; HEM45; ISG20
UniProt ID: (Human) Q96AZ6, (Mouse) Q9JL16
Entrez Gene ID: (Human) 3669, (Mouse) 57444, (Rat) 293052
DNA catabolic process, exonucleolytic
rRNA processing
RNA catabolic process
cell proliferation
response to virus
negative regulation of viral genome replication
defense response to virus
type I interferon signaling pathway
RNA phosphodiester bond hydrolysis, exonucleolytic
immune system process
innate immune response
nucleic acid phosphodiester bond hydrolysis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support