Invitrogen

ISG20 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

3 Published Figures

4 References

View all (8) ISG20 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

ISG20 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 7

ISG20 Antibody (PA5-30073) in IHC (P)

Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using ISG20 (Product # PA5-30073) antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

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ISG20 Antibody — FIG 6 Generation of ISG20-resistant BUNV and demonstration that endogenous ISG20 possesses antibunyaviral activity. (A and B) Plaque phenotypes (on Vero and Vero-ISG20 cells) (A) and fold inhibition (B) of the parental virus and the P20 and P40 ISG20-resistant viruses by ISG20 (fold reductions in PFU per milliliter for comparisons of naive and Vero-ISG20 cells). (C) Comparison of growth curves for the parental virus and the P40 virus on Vero-ISG20 and naive Vero cells. Cells were infected at an MOI of 0.01 (data are means +- SD; n = 3). (D) Infectious yields (36 h after infection at an MOI of 0.01) of wild-type (wt) BUNV and reassortant mutants (mutant segments are denoted by "'") from Vero-ISG20 or Vero-D94G cells (data are means and ranges; n = 2). (E) Biological IFN assays to assess the ability of supernatants from HeLa cells infected with the indicated viruses to inhibit the replication of EMCV in A549-Npro cells. (F) Western blot analysis of basal and inducible expression in naive HeLa cells, HeLa-shGFP cells, and HeLa-shISG20 cells. Basal expression or universal interferon (500 U/mL)-induced ISG20 expression was stably detected in naive HeLa cells and HeLa-shGFP cells, whereas both basal and inducible ISG20 expression was undetectable in HeLa-shISG20 cells. (G) Growth curves for parental BUNV, P40 Vero-EMPTY-passaged BUNV, and P40 ISG20-resistant BUNV on HeLa cells (MOI = 0.01) (data are means +- SD; n = 3). (H) Growth curves for parental BUNV and P40 ISG20-resistant {TM}

Product Details

PA5-30073

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:1,000

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 181 of Human ISG20

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.1M tris glycine, pH 7, with 20% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2547547

Product Specific Information

Recommended positive controls: Raji, mouse spleen.

Predicted reactivity: Mouse (83%), Rat (82%), Pig (81%), Rhesus Monkey (97%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Interferon-induced antiviral exoribonuclease that acts on single-stranded RNA and also has minor activity towards single-stranded DNA. Exhibits antiviral activity against RNA viruses including hepatitis C virus (HCV), hepatitis A virus (HAV) and yellow fever virus (YFV) in an exonuclease-dependent manner. May also play additional roles in the maturation of snRNAs and rRNAs, and in ribosome biogenesis. [UniProt]

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

USP22 controls type III interferon signaling and SARS-CoV-2 infection through activation of STING.

Cell death & disease

Karlowitz R,Stanifer ML,Roedig J,Andrieux G,Bojkova D,Bechtel M,Smith S,Kowald L,Schubert R,Boerries M,Cinatl J,Boulant S,van Wijk SJL

PA5-30073 was used in Western Blotting to reveal USP22 as central host regulator of STING and type III IFN signaling, with important implications for SARS-CoV-2 infection and antiviral defense.

Sat Aug 06 00:00:00 EDT 2022

USP22 controls type III interferon signaling and SARS-CoV-2 infection through activation of STING.

Cell death & disease

Karlowitz R,Stanifer ML,Roedig J,Andrieux G,Bojkova D,Bechtel M,Smith S,Kowald L,Schubert R,Boerries M,Cinatl J,Boulant S,van Wijk SJL

PA5-30073 was used in Western Blotting to reveal USP22 as central host regulator of STING and type III IFN signaling, with important implications for SARS-CoV-2 infection and antiviral defense.

Sat Aug 06 00:00:00 EDT 2022

ISG15-dependent activation of the sensor MDA5 is antagonized by the SARS-CoV-2 papain-like protease to evade host innate immunity.

Nature microbiology

Liu G,Lee JH,Parker ZM,Acharya D,Chiang JJ,van Gent M,Riedl W,Davis-Gardner ME,Wies E,Chiang C,Gack MU

PA5-30073 was used in Western Blot to investigate the role of interferon (IFN)-stimulated gene 15 in innate immunity.

Thu Apr 01 00:00:00 EDT 2021

ISG15-dependent Activation of the RNA Sensor MDA5 and its Antagonism by the SARS-CoV-2 papain-like protease.

bioRxiv : the preprint server for biology

Liu G,Lee JH,Parker ZM,Acharya D,Chiang JJ,van Gent M,Riedl W,Davis-Gardner ME,Wies E,Chiang C,Gack MU

PA5-30073 was used in Western Blotting to demonstrate interferon-stimulated gene ISG15 is crucial for the ability of viral RNA sensor MDA5 to perform an antiviral response.

Tue Oct 27 00:00:00 EDT 2020

Interferon-Stimulated Gene (ISG)-Expression Screening Reveals the Specific Antibunyaviral Activity of ISG20.

Journal of virology

Feng J,Wickenhagen A,Turnbull ML,Rezelj VV,Kreher F,Tilston-Lunel NL,Slack GS,Brennan B,Koudriakova E,Shaw AE,Rihn SJ,Rice CM,Bieniasz PD,Elliott RM,Shi X,Wilson SJ

Published figure using ISG20 polyclonal antibody (Product # PA5-30073) in Western Blot

Sun Jul 01 00:00:00 EDT 2018

Bioinformatics

Protein Aliases: Estrogen-regulated transcript 45 protein; interferon stimulated exonuclease; interferon stimulated exonuclease gene 20kDa; Interferon-stimulated gene 20 kDa protein; Promyelocytic leukemia nuclear body-associated protein ISG20; Protein DnaQL

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Gene Aliases: 1600023I01Rik; 2010107M23Rik; 20kDa; CD25; DnaQL; HEM45; ISG20

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UniProt ID: (Human) Q96AZ6, (Mouse) Q9JL16

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Entrez Gene ID: (Human) 3669, (Mouse) 57444

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Function(s)

3'-5'-exoribonuclease activity exonuclease activity single-stranded DNA 3'-5' exodeoxyribonuclease activity exoribonuclease II activity U1 snRNA binding U2 snRNA binding U3 snoRNA binding metal ion binding nucleic acid binding RNA binding nuclease activity hydrolase activity exoribonuclease

Process(es)

DNA catabolic process, exonucleolytic rRNA processing RNA catabolic process cell proliferation response to virus negative regulation of viral genome replication defense response to virus type I interferon signaling pathway RNA phosphodiester bond hydrolysis, exonucleolytic immune system process innate immune response

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ISG20 Polyclonal Antibody

ISG20 Antibody (PA5-30073) in IHC (P)

Product Details

PA5-30073

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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