Invitrogen

ISL1 Monoclonal Antibody (1H9)

ISL1 Antibody in Immunocytochemistry (ICC/IF)

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ISL1 Antibody (MA5-15515) in ICC/IF

Immunofluorescence analysis of HEK293 cells trasfected with full-length ISL1-human IgG Fc using ISL1 monoclonal antibody (Product # MA5-15515) (Green). Blue: DRAQ5 fluorescent DNA dye.

ISL1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ISL1 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

MA5-15515

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000

Immunocytochemistry (ICC/IF)

1:200-1:1,000

View 1 publication 1 publication

ELISA (ELISA)

1:10,000

Product Specifications

Species Reactivity

Human

Published species

Chimpanzee, Human, Non-human primate

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

1H9

Immunogen

Purified recombinant fragment of human ISL1 expressed in E. Coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.03% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_10975891

Product Specific Information

MA5-15515 targets ISL1 in indirect ELISA, IF, IHC, and WB applications and shows reactivity with Human samples.

The MA5-15515 immunogen is purified recombinant fragment of human ISL1 expressed in E. Coli.

MA5-15515 detects ISL1 which has a predicted molecular weight of approximately 39kDa.

Target Information

This gene encodes a member of the LIM/homeodomain family of transcription factors. The encoded protein binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. The encoded protein is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Mutations in this gene have been associated with maturity-onset diabetes of the young.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Comparison of induced neurons reveals slower structural and functional maturation in humans than in apes.

eLife

Schörnig M,Ju X,Fast L,Ebert S,Weigert A,Kanton S,Schaffer T,Nadif Kasri N,Treutlein B,Peter BM,Hevers W,Taverna E

MA5-15515 was used in Immunocytochemistry to conclude that human iNs develop slower than chimpanzee and bonobo iNs, and this difference in timing likely depends on functions downstream of NGN2.

Wed Jan 20 00:00:00 EST 2021

Comparison of induced neurons reveals slower structural and functional maturation in humans than in apes.

eLife

Schörnig M,Ju X,Fast L,Ebert S,Weigert A,Kanton S,Schaffer T,Nadif Kasri N,Treutlein B,Peter BM,Hevers W,Taverna E

MA5-15515 was used in Immunocytochemistry to conclude that human iNs develop slower than chimpanzee and bonobo iNs, and this difference in timing likely depends on functions downstream of NGN2.

Wed Jan 20 00:00:00 EST 2021

Comparison of induced neurons reveals slower structural and functional maturation in humans than in apes.

eLife

Schörnig M,Ju X,Fast L,Ebert S,Weigert A,Kanton S,Schaffer T,Nadif Kasri N,Treutlein B,Peter BM,Hevers W,Taverna E

MA5-15515 was used in Immunocytochemistry to conclude that human iNs develop slower than chimpanzee and bonobo iNs, and this difference in timing likely depends on functions downstream of NGN2.

Wed Jan 20 00:00:00 EST 2021

Herpes simplex virus type 1 infection leads to neurodevelopmental disorder-associated neuropathological changes.

PLoS pathogens

Qiao H,Guo M,Shang J,Zhao W,Wang Z,Liu N,Li B,Zhou Y,Wu Y,Chen P

MA5-15515 was used in Immunohistochemistry-immunofluorescence to study fetal brain development and the potential neuropathological effects induced by the HSV-1 infections.

Thu Oct 01 00:00:00 EDT 2020

Herpes simplex virus type 1 infection leads to neurodevelopmental disorder-associated neuropathological changes.

PLoS pathogens

Qiao H,Guo M,Shang J,Zhao W,Wang Z,Liu N,Li B,Zhou Y,Wu Y,Chen P

MA5-15515 was used in Immunohistochemistry-immunofluorescence to study fetal brain development and the potential neuropathological effects induced by the HSV-1 infections.

Thu Oct 01 00:00:00 EDT 2020

Neurodevelopmental impairment induced by prenatal valproic acid exposure shown with the human cortical organoid-on-a-chip model.

Microsystems & nanoengineering

Cui K,Wang Y,Zhu Y,Tao T,Yin F,Guo Y,Liu H,Li F,Wang P,Chen Y,Qin J

MA5-15515 was used in Immunohistochemistry to probe the effects of antiepileptic drug valproic acid exposure on the developing fetal brain at early stages using engineered cortical organoids from human induced pluripotent stem cells.

Wed Sep 29 00:00:00 EDT 2021

Neurodevelopmental impairment induced by prenatal valproic acid exposure shown with the human cortical organoid-on-a-chip model.

Microsystems & nanoengineering

Cui K,Wang Y,Zhu Y,Tao T,Yin F,Guo Y,Liu H,Li F,Wang P,Chen Y,Qin J

Wed Sep 29 00:00:00 EDT 2021

Engineering stem cell-derived 3D brain organoids in a perfusable organ-on-a-chip system.

RSC advances

Wang Y,Wang L,Guo Y,Zhu Y,Qin J

MA5-15515 was used in Immunohistochemistry to present a new strategy to generate hiPSCs-derived 3D brain organoids using an organ-on-a-chip system in a controlled manner.

Tue Jan 02 00:00:00 EST 2018

Engineering stem cell-derived 3D brain organoids in a perfusable organ-on-a-chip system.

RSC advances

Wang Y,Wang L,Guo Y,Zhu Y,Qin J

MA5-15515 was used in Immunohistochemistry to present a new strategy to generate hiPSCs-derived 3D brain organoids using an organ-on-a-chip system in a controlled manner.

Tue Jan 02 00:00:00 EST 2018

Bioinformatics

Protein Aliases: Insulin gene enhancer protein ISL-1; ISL1 transcription factor, LIM/homeodomain; Islet-1

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Gene Aliases: Isl-1; ISL1; ISLET1

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UniProt ID: (Human) P61371

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Entrez Gene ID: (Human) 3670

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Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II activating transcription factor binding RNA polymerase II transcription coactivator activity enhancer sequence-specific DNA binding chromatin binding protein binding zinc ion binding ligand-dependent nuclear receptor binding estrogen receptor binding bHLH transcription factor binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ISL1 Monoclonal Antibody (1H9)

ISL1 Antibody (MA5-15515) in ICC/IF

Product Details

MA5-15515

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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