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Invitrogen
ISL1 Recombinant Rabbit Monoclonal Antibody (ARC0511)
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 5
ISL1 Antibody (MA5-35062) in ICC/IF
Immunofluorescence analysis of Insulin gene enhancer protein ISL-1 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with ISL1 Recombinant Rabbit Monoclonal Antibody (ARC0511) (Product # MA5-35062) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adso... View More
Product Details
MA5-35062
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
ARC0511
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 200-300 of human Islet1 (P61371)
Conjugate
Form
Liquid
Concentration
0.51 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2848967
Product Specific Information
Immunogen sequence: YAANPRPDAL MKEQLVEMTG LSPRVIRVWF QNKRCKDKKR SIMMKQLQQQ QPNDKTNIQG MTGTPMVAAS PERHDGGLQA NPVEVQSYQP PWKVLSDFAL Q
Target Information
This gene encodes a member of the LIM/homeodomain family of transcription factors. The encoded protein binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. The encoded protein is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Mutations in this gene have been associated with maturity-onset diabetes of the young.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Insulin gene enhancer protein ISL-1; insulin related protein; isl-1 homeobox; ISL1 transcription factor LIM/homeodomain (islet-1); ISL1 transcription factor, LIM/homeodomain; ISL1 transcription factor, LIM/homeodomain 1; Islet 1; Islet-1; Lim-homeodomain protein Islet1
Gene Aliases: Isl-1; isl-1=homeobox; ISL1; ISLET1
UniProt ID: (Human) P61371, (Mouse) P61372, (Rat) P61374
Entrez Gene ID: (Human) 3670, (Mouse) 16392, (Rat) 64444
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
RNA polymerase II activating transcription factor binding
RNA polymerase II transcription coactivator activity
enhancer sequence-specific DNA binding
chromatin binding
protein binding
zinc ion binding
ligand-dependent nuclear receptor binding
estrogen receptor binding
bHLH transcription factor binding
DNA binding
sequence-specific DNA binding
metal ion binding
negative regulation of transcription from RNA polymerase II promoter
neural crest cell migration
heart morphogenesis
secondary heart field specification
outflow tract septum morphogenesis
outflow tract morphogenesis
endocardial cushion morphogenesis
cardiac right ventricle morphogenesis
regulation of secondary heart field cardioblast proliferation
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
multicellular organism development
heart development
positive regulation of cell proliferation
regulation of gene expression
positive regulation of vascular endothelial growth factor production
spinal cord motor neuron cell fate specification
spinal cord motor neuron differentiation
visceral motor neuron differentiation
trigeminal nerve development
pituitary gland development
neuron differentiation
pancreas development
retinal ganglion cell axon guidance
positive regulation of insulin secretion
positive regulation of granulocyte macrophage colony-stimulating factor production
positive regulation of interferon-gamma production
positive regulation of interleukin-1 alpha production
positive regulation of interleukin-1 beta production
positive regulation of interleukin-12 production
positive regulation of interleukin-6 production
positive regulation of tumor necrosis factor production
negative regulation of intracellular estrogen receptor signaling pathway
positive regulation of tyrosine phosphorylation of Stat3 protein
positive regulation of DNA binding
negative regulation of neuron apoptotic process
negative regulation of neuron differentiation
positive regulation of angiogenesis
positive regulation of transcription from RNA polymerase II promoter
neuron fate commitment
neuron fate specification
mesenchymal cell differentiation
sensory system development
peripheral nervous system neuron development
peripheral nervous system neuron axonogenesis
negative regulation of inflammatory response
ventricular cardiac muscle tissue morphogenesis
pharyngeal system development
cardiac muscle cell myoblast differentiation
innervation
atrial septum morphogenesis
cardiac cell fate determination
positive regulation of granulocyte colony-stimulating factor production
negative regulation of protein homodimerization activity
negative regulation of canonical Wnt signaling pathway
positive regulation of macrophage colony-stimulating factor production
regulation of transcription from RNA polymerase II promoter
axon regeneration
positive regulation of histone acetylation
cellular response to glucocorticoid stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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