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Invitrogen
ITGA2 Polyclonal Antibody
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 5
ITGA2 Antibody (PA5-47193) in ICC/IF
Immunofluorescence analysis of CD49b/ITGA2 was performed using 70% confluent log phase U-2 OS cells. The cells were fixed with 4% paraformaldehyde for 10 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with CD49b/ITGA2 Sheep Polyclonal Antibody (Product # PA5-47193) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Donkey anti-Sheep IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A-11015) at a dilution of 1:2000 for 45 minutes at r... View More
Product Details
PA5-47193
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Mouse
Host/Isotype
Sheep
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Integrin alpha 2/CD49b Tyr27-Thr1129
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2607349
Product Specific Information
In direct ELISAs and Western blots, approximately10% cross-reactivity with recombinant human Integrin alpha M is observed and less than 1% cross-reactivity with recombinant mouse (rm) Integrin alpha 5 and rmIntegrin alpha E is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
CD49b (Integrin alpha 2, Integrin alpha 2 chain, VLA-2 alpha chain, HM alpha 2) is a member of the integrin family. It is a glycoprotein with molecular weight of 150 kD, and it complexes with CD29 (Integrin beta 1) to form the heterodimeric integrin VLA-2 (integrin alpha 2 beta 1, or GPIa/IIa) complex. VLA-2 is an extracellular receptor for laminin, collagen, and fibronectin, and interaction with its ligands results in the activation of intracellular signaling pathways. It has reported roles in VEGF-induced angiogenesis in vivo, as well as adhesion and lymphocyte activation. CD49b is expressed by NK cells, NK-T cells, monocytes, platelets, and epithelial cells. It is also expressed on adaptive immune cells such as T and B cells, specifically on a subset of CD4+ T cells in the spleen, on intraepithelial and lamina propria lymphocytes in the intestine, as well as on a population of peripheral CD4+ type 1 T regulatory (Tr1) cells that co-express LAG-3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: alpha 2 subunit of VLA-2 receptor; BDPLT9; CD49 antigen-like family member B; CD49b; Collagen receptor; GPIa; human platelet alloantigen system 5; Integrin alpha-2; integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor); platelet antigen Br; platelet glycoprotein GPIa; Platelet membrane glycoprotein Ia; very late activation protein 2 receptor, alpha-2 subunit; VLA-2 receptor, alpha 2 subunit; VLA-2 subunit alpha; VLA2 receptor
Gene Aliases: BR; CD49B; DX5; GPIa; HPA-5; ITGA2; VLA-2; VLAA2
UniProt ID: (Human) P17301, (Mouse) Q62469
Entrez Gene ID: (Human) 3673, (Mouse) 16398
response to hypoxia
positive regulation of leukocyte migration
substrate-dependent cell migration
hypotonic response
cell adhesion
cell-matrix adhesion
integrin-mediated signaling pathway
female pregnancy
blood coagulation
cell proliferation
organ morphogenesis
positive regulation of epithelial cell migration
positive regulation of alkaline phosphatase activity
response to amine
response to muscle activity
positive regulation of smooth muscle cell migration
extracellular matrix organization
mammary gland development
positive regulation of cell projection organization
cell-substrate adhesion
positive regulation of collagen biosynthetic process
positive regulation of collagen binding
response to L-ascorbic acid
cell adhesion mediated by integrin
collagen-activated signaling pathway
response to drug
positive regulation of DNA binding
skin morphogenesis
establishment of protein localization
positive regulation of translation
positive regulation of cell adhesion
positive regulation of smooth muscle contraction
viral entry into host cell
focal adhesion assembly
mesodermal cell differentiation
positive regulation of smooth muscle cell proliferation
positive regulation of inflammatory response
positive regulation of positive chemotaxis
detection of mechanical stimulus involved in sensory perception of pain
positive regulation of transmission of nerve impulse
positive regulation of phagocytosis, engulfment
hepatocyte differentiation
response to parathyroid hormone
cellular response to mechanical stimulus
cellular response to estradiol stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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