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Invitrogen
ITGA4 Polyclonal Antibody
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 4
ITGA4 Antibody (PA5-20595) in WB
Western blot was performed using Anti-ITGA4 Polyclonal Antibody (Product # PA5-20595) and 160, 150 kDa bands corresponding to ITGA4 were observed. Membrane enriched extracts (30 µg lysate) of Jurkat (Lane 1), MOLT-4 (Lane 2), HEL 92.1.7 (Lane 3), THP-1 (Lane 4), Hep G2 (Lane 5), MCF7 (Lane 6) and K-562 (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The... View More
Product Details
PA5-20595
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Not Applicable
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A 13 amino acid peptide from near the carboxy terminus of human Integrin alpha 4.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_11153230
Product Specific Information
Despite its predicted molecular weight, Integrin alpha4 often migrates at a higher molecular weight in SDS-PAGE. A suggested positive control is Jurkat cell lysate.
PA5-20595 can be used with blocking peptide PEP-0715.
Target Information
The integrin alpha 4 (also known as CD49d and ITGA4) belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha and beta chains. Alpha4 chain associates with either beta1 or beta7 chain. It has been demonstrated that the putative ligand-binding sites of both integrin alpha4beta1 and alpha4beta7 is located on the alpha4 chain. These ligands included Madcam, VCAM, and fibronectin. Madcam is known as the principal ligand for integrin alpha4beta7. Recently it was also demonstrated that HIV-1 envelope can mimic Madcam by binding to and signaling though integrin alpha4beta7, the gut mucosal homing receptor for peripheral T cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 269C wild type; alpha 4 subunit of VLA-4 receptor; antigen CD49D, alpha-4 subunit of VLA-4 receptor; CD49 antigen-like family member D; CD49d; Integrin alpha-4; Integrin alpha-IV; integrin alpha4B; ITGA-IV; LPAM subunit alpha; Lymphocyte Peyer patch adhesion molecules subunit alpha; very late activation protein 4 receptor, alpha 4 subunit; VLA-4 receptor, alpha 4 subunit; VLA-4 subunit alpha
Gene Aliases: CD49D; IA4; ITGA4; Itga4B
UniProt ID: (Human) P13612, (Mouse) Q00651
Entrez Gene ID: (Human) 3676, (Rat) 311144, (Mouse) 16401
cell-matrix adhesion involved in ameboidal cell migration
leukocyte cell-cell adhesion
cell-matrix adhesion
integrin-mediated signaling pathway
B cell differentiation
extracellular matrix organization
heterotypic cell-cell adhesion
substrate adhesion-dependent cell spreading
endodermal cell differentiation
receptor clustering
regulation of immune response
leukocyte migration
leukocyte tethering or rolling
diapedesis
cellular response to cytokine stimulus
negative regulation of protein homodimerization activity
import into cell
positive regulation of leukocyte tethering or rolling
clathrin-mediated extracellular exosome endocytosis
positive regulation of T cell migration
blood vessel remodeling
positive regulation of leukocyte migration
cell adhesion
heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules
heart development
cell migration
face development
chorio-allantoic fusion
T cell migration
positive regulation of leukocyte cell-cell adhesion
single organismal cell-cell adhesion
mesenchyme development
visceral serous pericardium development
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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