Invitrogen

ITGB1 Monoclonal Antibody (MEM-101A), APC

1 Published Figure
2 References
View all (125) Integrin beta 1 (CD29) antibodies
Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

MA1-19458

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

10 µL x 100 µL whole blood or 10^6 cells
View 2 publications 2 publications
Product Specifications

Species Reactivity

Dog, Human, Pig

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

MEM-101A

Immunogen

Burkitt's lymphoma cell line Raji.
View immunogen

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra spectra

Form

Liquid

Purification

Size-exclusion chromatography

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

15mM sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1075422

Product Specific Information

This antibody reacts with an extracellular epitope of CD29 (integrin beta1 chain), a 130 kDa single chain type I glycoprotein expressed as a heterodimer (non-covalently associated with the integrin alpha subunits 1-6). CD29 is broadly expressed on majority of hematopoietic and non-hematopoietic cells (leukocytes, platelets, fibroblasts, endothelial cells, epithelial cells and mast cells).

This antibody will not cross-react with mouse.

Target Information

ITGB1 (Integrin Subunit Beta 1, beta1 integrin subunit, GPIIa, CD29) is a 110 kDa cell surface glycoprotein that is widely expressed by a variety of cells including all leucocytes. ITGB1 forms non-covalently linked heterodimers with at least 6 different alpha chains (alpha1-alpha6, CDa-f) determining the binding properties of beta1 (VLA) integrins. ITGB1 is a cell adhesion molecule appearing on platelets, as the common Beta subunit of the very late activation antigen (VLA), and as a component of various protein complexes binding to extracellular matrix proteins. Decreased expression of ITGB1 correlates with acquiring multidrug resistance of tumor cells in the presence of anti-tumor drug. ITGB1 is up-regulated in leukocytes during emigration and extravascular migration and appear to be critically involved in regulating the immune cell trafficking from blood to tissue. Further, ITGB1 also regulates tissue damage and disease symptoms related to inflammatory bowel disease. Through an ITGB1-dependent mechanism, fibronectin and type I collagen enhance cytokine secretion of human airway smooth muscle in response to IL-1beta. More than 8 beta subunits with numerous splice variant isoforms have been identified in mammals. There are two major forms of integrin beta1: beta1A and beta1D, which differ in 13 amino acids. The distribution pattern in adult tissues for integrin beta types are mutually exclusive. Beta1A is present in all tissues, except cardiac and skeletal muscle which express the beta1D variant.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CD29; Fibronectin receptor subunit beta; Glycoprotein IIa; GPIIA; integrin beta 1; Integrin beta-1; integrin beta-1 subunit; Integrin beta1; integrin VLA-4 beta subunit; integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12); ITBG1D; very late activation protein, beta polypeptide; VLA-4 subunit beta

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Gene Aliases: CD29; FNRB; GPIIA; ITGB1; MDF2; MSK12; VLA-BETA; VLAB

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UniProt ID: (Pig) Q9GLP0, (Human) P05556

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Entrez Gene ID: (Pig) 397019, (Human) 3688, (Dog) 477956

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Function(s)
signaling receptor activity metal ion binding protein heterodimerization activity virus receptor activity glycoprotein binding fibronectin binding protease binding actin binding integrin binding protein binding coreceptor activity protein kinase binding protein domain specific binding C-X3-C chemokine binding protein complex binding peptide binding laminin binding cell adhesion molecule binding alpha-actinin binding collagen binding involved in cell-matrix adhesion cadherin binding involved in cell-cell adhesion integrin
Process(es)
regulation of collagen catabolic process positive regulation of cell migration receptor internalization cell adhesion mediated by integrin cellular response to low-density lipoprotein particle stimulus positive regulation of protein localization to plasma membrane G1/S transition of mitotic cell cycle in utero embryonic development cell fate specification tissue homeostasis cell migration involved in sprouting angiogenesis cellular calcium ion homeostasis cellular defense response homophilic cell adhesion via plasma membrane adhesion molecules leukocyte cell-cell adhesion cell-matrix adhesion calcium-independent cell-matrix adhesion transforming growth factor beta receptor signaling pathway integrin-mediated signaling pathway regulation of G-protein coupled receptor protein signaling pathway positive regulation of cell proliferation negative regulation of cell proliferation germ cell migration visual learning positive regulation of cell-substrate adhesion positive regulation of neuron projection development response to activity cell migration formation of radial glial scaffolds B cell differentiation extracellular matrix organization negative regulation of cell projection organization cell-substrate adhesion protein transport within lipid bilayer cell-cell adhesion mediated by integrin heterotypic cell-cell adhesion response to gonadotropin negative regulation of Rho protein signal transduction response to drug positive regulation of apoptotic process stress fiber assembly positive regulation of MAPK cascade positive regulation of GTPase activity sarcomere organization negative regulation of neuron differentiation positive regulation of endocytosis viral entry into host cell mesodermal cell differentiation axon extension dendrite morphogenesis positive regulation of peptidyl-tyrosine phosphorylation regulation of immune response leukocyte migration leukocyte tethering or rolling regulation of cell cycle cardiac muscle cell differentiation maternal process involved in female pregnancy bicellular tight junction assembly cellular response to mechanical stimulus cellular response to vitamin D cellular response to ionizing radiation positive regulation of establishment of protein localization to plasma membrane negative regulation of anoikis
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