Invitrogen

ITGB1 Monoclonal Antibody (TS2/16)

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

3 Published Figures

22 References

View all (125) Integrin beta 1 (CD29) antibodies

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ITGB1 Antibody (MA2910) in Flow

Flow cytometry analysis of CD29 in peripheral blood granulocytes compared to an isotype control (blue). Human blood was collected, combined with a hydrophilic polysaccharide, centrifuged, transferred to a conical tube and washed with PBS. 50 µL of cell solution was added to each tube at a dilution of 2x10^7 cells/mL, followed by the addition of 50 µL of isotype control and primary antibody (Product # MA2910) at a dilution of 0.5 µg/test. Cells were incubated for 30 min at 4ºC and washed with a cell buffer, followed by incubation with a DyL... View More

ITGB1 Antibody in T-Cell Activation (TCA)

Product Image Gallery

ITGB1 Antibody — Antibody clone (TS2/16) specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. Lossof signal was observed for target protein in TS2/16 KOcells (blue histogram) compared to the control Cas9cells (pink histogram) using CD29 antibody (TS2/16). Yellow histogram represents staining with the isotype control. {KO}

Product Details

MA2910

Applications

Tested Dilution

Publications

Western Blot (WB)

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.5 µg/test

View 2 publications 2 publications

ELISA (ELISA)

View 1 publication 1 publication

Immunoprecipitation (IP)

View 1 publication 1 publication

Neutralization (Neu)

View 12 publications 12 publications

Immunomicroscopy (IM)

View 1 publication 1 publication

Inhibition Assays (IA)

View 1 publication 1 publication

T-Cell Activation (TCA)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

TS2/16

Immunogen

Human Beta1 Integrin, the Beta1 chain of the Beta1 integrin heterodimer (CD29)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_223515

Product Specific Information

MA2910 targets CD29 in FACS applications and shows reactivity with Human samples.

The MA2910 immunogen is human Beta1 Integrin, the Beta1 chain of the Beta1 integrin heterodimer (CD29).

MA2910 detects CD29 which has a predicted molecular weight of approximately 88 kDa.

This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).

Target Information

ITGB1 (Integrin Subunit Beta 1, beta1 integrin subunit, GPIIa, CD29) is a 110 kDa cell surface glycoprotein that is widely expressed by a variety of cells including all leucocytes. ITGB1 forms non-covalently linked heterodimers with at least 6 different alpha chains (alpha1-alpha6, CDa-f) determining the binding properties of beta1 (VLA) integrins. ITGB1 is a cell adhesion molecule appearing on platelets, as the common Beta subunit of the very late activation antigen (VLA), and as a component of various protein complexes binding to extracellular matrix proteins. Decreased expression of ITGB1 correlates with acquiring multidrug resistance of tumor cells in the presence of anti-tumor drug. ITGB1 is up-regulated in leukocytes during emigration and extravascular migration and appear to be critically involved in regulating the immune cell trafficking from blood to tissue. Further, ITGB1 also regulates tissue damage and disease symptoms related to inflammatory bowel disease. Through an ITGB1-dependent mechanism, fibronectin and type I collagen enhance cytokine secretion of human airway smooth muscle in response to IL-1beta. More than 8 beta subunits with numerous splice variant isoforms have been identified in mammals. There are two major forms of integrin beta1: beta1A and beta1D, which differ in 13 amino acids. The distribution pattern in adult tissues for integrin beta types are mutually exclusive. Beta1A is present in all tissues, except cardiac and skeletal muscle which express the beta1D variant.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Human Adult Astrocyte Extracellular Vesicle Transcriptomics Study Identifies Specific RNAs Which Are Preferentially Secreted as EV Luminal Cargo.

Genes

Shanthi KB,Fischer D,Sharma A,Kiviniemi A,Kaakinen M,Vainio SJ,Bart G

MA2910 was used in Immunomicroscopy to characterize the secreted extracellular vesicles and RNA cargo of human astrocytes derived from an adult brain.

Fri Mar 31 00:00:00 EDT 2023

Blood vessels guide Schwann cell migration in the adult demyelinated CNS through Eph/ephrin signaling.

Acta neuropathologica

Garcia-Diaz B,Bachelin C,Coulpier F,Gerschenfeld G,Deboux C,Zujovic V,Charnay P,Topilko P,Baron-Van Evercooren A

Published figure using ITGB1 monoclonal antibody (Product # MA2910) in Western Blot

Sun Sep 01 00:00:00 EDT 2019

Blood vessels guide Schwann cell migration in the adult demyelinated CNS through Eph/ephrin signaling.

Acta neuropathologica

Garcia-Diaz B,Bachelin C,Coulpier F,Gerschenfeld G,Deboux C,Zujovic V,Charnay P,Topilko P,Baron-Van Evercooren A

Published figure using ITGB1 monoclonal antibody (Product # MA2910) in Western Blot

Sun Sep 01 00:00:00 EDT 2019

PI3Kβ links integrin activation and PI(3,4)P₂ production during invadopodial maturation.

Molecular biology of the cell

Erami Z,Heitz S,Bresnick AR,Backer JM

Published figure using ITGB1 monoclonal antibody (Product # MA2910) in T-Cell Activation

Thu Aug 15 00:00:00 EDT 2019

The Phosphorylation and Distribution of Cortactin Downstream of Integrin α9β1 Affects Cancer Cell Behaviour.

Scientific reports

Høye AM,Couchman JR,Wewer UM,Yoneda A

MA2910 was used in blocking or activating experiment investigate the interaction between cortactin and alpha9beta1 integrin

Fri Jun 24 00:00:00 EDT 2016

β1 integrin regulates Arg to promote invadopodial maturation and matrix degradation.

Molecular biology of the cell

Beaty BT,Sharma VP,Bravo-Cordero JJ,Simpson MA,Eddy RJ,Koleske AJ,Condeelis J

Published figure using ITGB1 monoclonal antibody (Product # MA2910) in T-Cell Activation

Sat Jun 01 00:00:00 EDT 2013

Roles of focal adhesions and fibronectin-mediated cohesion in proliferation of confluent fibroblasts.

Journal of cellular physiology

Tanaka M,Abe T,Hara Y

MA2910 was used in blocking/activating experiment to study the roles of focal adhesion and intercellular cohesion in the proliferation of normal human fibroblasts

Wed Apr 01 00:00:00 EDT 2009

Distribution of costameric proteins in the diaphragm of patients with chronic obstructive pulmonary disease.

Respiration; international review of thoracic diseases

Wijnhoven JH,Hafmans T,Dekhuijzen PN

MA2910 was used in immunohistochemistry to study the expression of beta 1 integrin in the diaphragm of COPD and non-COPD patients

Tue Nov 14 00:00:00 EST 2006

PLCgamma1 is essential for early events in integrin signalling required for cell motility.

Journal of cell science

Jones NP,Peak J,Brader S,Eccles SA,Katan M

MA2910 was used in blocking/activating experiment to study the role of PLC gamma 1 in cell motility signalling

Wed Jun 15 00:00:00 EDT 2005

Collective and single-molecule interactions of alpha5beta1 integrins.

Langmuir : the ACS journal of surfaces and colloids

Kokkoli E,Ochsenhirt SE,Tirrell M

MA2910 was used in blocking/activating experiment to investigate the collective and single-molecule interactions between alpha5beta1 receptor and GRGDSP ligand

Tue Mar 16 00:00:00 EST 2004

Chemotaxis of non-compressed blood polymorphonuclear leukocytes from an adolescent with severe leukocyte adhesion deficiency.

American journal of hematology

Malawista SE,de Boisfleury Chevance A,Brown EJ,Boxer LA,Law SK

MA2910 was used in blocking/activating experiment to study the CD18 mutation in a patient with severe leukocyte adhesion deficiency

Sun Jun 01 00:00:00 EDT 2003

Syndecan-1-mediated cell spreading requires signaling by alphavbeta3 integrins in human breast carcinoma cells.

Experimental cell research

Beauvais DM,Rapraeger AC

MA2910 was used in blocking/activating experiment to study the role of alphavbeta3 integrin in syndecan-1-mediated cell spreading

Tue Jun 10 00:00:00 EDT 2003

Actin-rich spherical extrusion induced in okadaic acid-treated K562 cells by crosslinking of membrane microdomains.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society

Baba T,Udaka K,Terada N,Ueda H,Fujii Y,Ohno S,Sato SB

MA2910 was used in blocking/activating experiment to study the effect of membrane microdomain crosslinkingon actin-rich spherical extrusion in OKA-treated cells

Sat Feb 01 00:00:00 EST 2003

EGFR is a transducer of the urokinase receptor initiated signal that is required for in vivo growth of a human carcinoma.

Cancer cell

Liu D,Aguirre Ghiso J,Estrada Y,Ossowski L

MA2910 was used in immunoprecipitation to study the role of EGFR in the signal transduction pathway for human carcinoma growth

Sat Jun 01 00:00:00 EDT 2002

Adhesion of alpha5beta1 receptors to biomimetic substrates constructed from peptide amphiphiles.

Biomaterials

Dillo AK,Ochsenhirt SE,McCarthy JB,Fields GB,Tirrell M

MA2910 was used in ELISA to evaluate the utility of peptide amphiphiles

Fri Jun 01 00:00:00 EDT 2001

Extracellular matrix regulates induction of alkaline phosphatase expression by ascorbic acid in human fibroblasts.

Journal of cellular physiology

Abe T,Abe Y,Aida Y,Hara Y,Maeda K

MA2910 was used in blocking/activating experiment to investigate the role of extracellular matrix for induction of alkaline phosphatase expression by ascorbic acid in human fibroblasts

Thu Nov 01 00:00:00 EST 2001

The chemokine receptor CXCR4 enhances integrin-mediated in vitro adhesion and facilitates engraftment of leukemic precursor-B cells in the bone marrow.

Experimental hematology

Shen W,Bendall LJ,Gottlieb DJ,Bradstock KF

MA2910 was used in flow cytometry to investigate the role of SDF-1 and CXCR4 in regulation of beta 1 integrin function

Sat Dec 01 00:00:00 EST 2001

Yersinia enterocolitica invasin protein triggers IL-8 production in epithelial cells via activation of Rel p65-p65 homodimers.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Schulte R,Grassl GA,Preger S,Fessele S,Jacobi CA,Schaller M,Nelson PJ,Autenrieth IB

MA2910 was used in blocking/activating experiment to study the interleukin 8 production by HeLa cells incubated with anti-beta1 integrin monoclonal antibody

Tue Aug 01 00:00:00 EDT 2000

A role for caveolin and the urokinase receptor in integrin-mediated adhesion and signaling.

The Journal of cell biology

Wei Y,Yang X,Liu Q,Wilkins JA,Chapman HA

MA2910 was used in blocking/activating experiment to study integrin-mediated adhesion

Mon Mar 22 00:00:00 EST 1999

Heterologous desensitization of IL-8-mediated chemotaxis in human neutrophils by a cell-binding fragment of fibronectin.

Journal of leukocyte biology

Stanton KJ,Frewin MB,Gudewicz PW

MA2910 was used in blocking/activating experiment to study the effect of fibronectin fragment on chemotaxis in human neutrophils

Thu Apr 01 00:00:00 EST 1999

Influence of adhesion molecule expression by human brain microvessel endothelium on cancer cell adhesion.

Journal of neuroimmunology

Brayton J,Qing Z,Hart MN,VanGilder JC,Fabry Z

MA2910 was used in flow cytometry to study the effect of adhesion molecule expression on cancer cell adhesion

Fri Aug 14 00:00:00 EDT 1998

Adhesion to vascular cell adhesion molecule 1 and fibronectin. Comparison of alpha 4 beta 1 (VLA-4) and alpha 4 beta 7 on the human B cell line JY.

The Journal of biological chemistry

Chan BM,Elices MJ,Murphy E,Hemler ME

MA2910 was used in blocking/activating experiment to investigate the importance of beta 1 in the functions of integrin VLA-4

Sat Apr 25 00:00:00 EDT 1992

Bioinformatics

Protein Aliases: CD29; Fibronectin receptor subunit beta; Glycoprotein IIa; GPIIA; integrin beta 1; Integrin beta-1; Integrin beta1; integrin VLA-4 beta subunit; integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12); ITBG1D; very late activation protein, beta polypeptide; VLA-4 subunit beta

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Gene Aliases: CD29; FNRB; GPIIA; ITGB1; MDF2; MSK12; VLA-BETA; VLAB

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UniProt ID: (Human) P05556

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Entrez Gene ID: (Human) 3688

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Function(s)

virus receptor activity glycoprotein binding fibronectin binding protease binding actin binding integrin binding protein binding coreceptor activity protein kinase binding protein domain specific binding C-X3-C chemokine binding protein complex binding peptide binding laminin binding metal ion binding protein heterodimerization activity cell adhesion molecule binding alpha-actinin binding collagen binding involved in cell-matrix adhesion cadherin binding involved in cell-cell adhesion integrin

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ITGB1 Monoclonal Antibody (TS2/16)

ITGB1 Antibody (MA2910) in Flow

Product Details

MA2910

Western Blot (WB)

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

ELISA (ELISA)

Immunoprecipitation (IP)

Neutralization (Neu)

Immunomicroscopy (IM)

Inhibition Assays (IA)

T-Cell Activation (TCA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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