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The specificity of the antibody was determined by measuring the cross-reactivity in ELISA for a range of compounds., , , , Cross Reactivity, Compound tested % Cross- reactivity, Ibuprofen 100%, Bezafibrate 0.4%, Gemfibrozil 0.2%, Bisphenol A 0.6%, Carazolol <0.1%, Metoprolol 0.1%, Chlorpyrifos 0.3%, Naproxen 2.2%, Clofibrinic acid 0.3%, Nonylphenol 1.3%, Diclofenac <0.1%, Octylphenol 0.6%, Erythromycine 0.1%, Propanolol 0.1%, Fenitrothion 0.5%, Vinclozolin <0.1%, Fenofibrate 0.5%, Zearalenon 0.2%.
The antibody may be used in ELISA and Immuno-affinity-chromatography (IAC). Suggested dilution 1:300 in ELISA (this titer is defined as the dilution that gives 50 % of the absorbance from the maximum absorbance when tested with ELISA). Plates are coated with 400 ng/mL ovalbumine-conjugated derivative. HRP-conjugated anti-rabbit IgG should be used as a tracer in a 1:8,000 dilution.
Store at 4 °C or -20°C. The working antibody solution is stable for at least 7 days at 4°C. Precautions should be taken for storage for longer periods. Problems of long term stability may occur with highly diluted solutions. No other preservative agent has been added to the present formulation. For long storage purposes in solution the addition of sodium azide to 0.02 % is advised with the appropriate precautions of use.
Ibuprofen is an anti-inflammatory drug used for pain relief, fever reduction and against swelling. It is one of the most sold over-the-counter drugs. It is found in the environment, especially in the water environment in the neighbourhood of large cities and hospitals.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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