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Invitrogen
IgE Monoclonal Antibody (23G3), Biotin, eBioscience™
FIGURE: 1 / 3
IgE Antibody (13-5992-82) in ELISA
Frontiers in cellular neuroscience 2020 -
Figure 2 Physical growth and adaptive immunity development during BLG sensitization. (A) Weights of mice were recorded during Weeks 1, 6, and 7 of sensitization to assess potential impact of the sensitization regime on overall health and growth. (B,C) Serum isolated from the terminal blood was used to quantify the levels of BLG-specific IgE (B) or IgG1 (C) using ELISA. Values shown in the graphs ind... View More
Product Details
13-5992-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rat
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
23G3
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_466857
Product Specific Information
Description: The 23G3 monoclonal antibody reacts with the epsilon heavy chain of mouse IgE. It does not react with other classes of mouse immunoglobulin including IgD, IgG, IgA, or IgM.
Applications Reported: The 23G3 antibody has been reported for use in flow cytometric analysis, and ELISA.
Applications Tested: The 23G3 antibody has been tested by ELISA and can be used at 1:5000-1:10000. This 23G3 antibody has also been tested by flow cytometric analysis of IGEL cells, and can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
IgE is one of the five classes of immunoglobulins found in mammals. Monomers consist of two heavy (epsilon) chains with 4 Ig-like constant domains, and two light chains. IgE plays an essential role in type I hypersensitivity (allergic responses such as hay fever, asthma, hives, anaphylatic shock) by binding to Fc receptors on basophils and mast cells. Crosslinking of IgE bound to Fc receptors induces degranulation of mast cells and basophils that promote allergic manifestations. IgE is also functionally important for immunity against parasites.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Immunoglobulin E; ImmunoglobulinE
Gene Aliases: Gm900
Entrez Gene ID: (Mouse) 380792
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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