Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
IkB alpha Polyclonal Antibody
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 5
IkB alpha Antibody (PA5-17888) in WB
Western blot was performed using Anti-IkB alpha Polyclonal Antibody (Product # PA5-17888) and a 36 kDa band corresponding to NF-kappa-B inhibitor alpha was observed across cell lines and tissue tested, along with down regulation of protein upon TNF alpha treatment in HeLa and LNCaP. Also, the protein expression was restored In LNCaP upon MG132 treatment. Nuclear enriched extracts (30 µg lysate) of HeLa (Lane 1), HeLa treated with TNF alpha (20 ng/mL for 10 min) (Lane 2), NIH/3T3 (Lane 3), NIH/3T3 treated with TNF alpha (20 ng/mL for 10 min... View More
Product Details
PA5-17888
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Dog,
Human,
Mouse,
Non-human primate,
Pig,
Rat
Published species
Not Applicable
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide surrounding Arg29 of human IkB alpha.
Conjugate
Form
Liquid
Concentration
50 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10982252
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
IkB-alpha is a 40 kDa protein that functions to inhibit NF- kappaB activity. The inhibition occurs via protein-protein interaction between I kappaB proteins and NF- kappaB dimers in the cytosol. The interaction of I kappa B-alpha with NF- kappaB masks the nuclear localization sequence of NF- kappaB, preventing NF- kappaB translocation to the nucleus. A variety of stimuli can activate gene expression by liberating NF- kappaB through the degradation of I kappaB alpha. These stimuli include the proinflammatory cytokines TNF- alpha and IL-1 beta, chemokines, PMA, growth factors, LPS, UV irradiation, viral infection, as well as various chemical and physical stresses. In humans, the gene is located on the q arm of chromosome 14. Activation of NFkB requires that IkB be phosphorylated on specific serine residues, which results in targeted degradation of IkB. IkB kinase alpha (IKK alpha), previously designated CHUK, interacts with IkB-alpha and specifically phosphorylates IkB-alpha on the sites that trigger its degradation Serines 32 and 36. IKK alpha appears to be critical for NFkB activation in response to proinflammatory cytokines. Phosphorylation of IkB by IKK alpha is stimulated by the NFkB inducing kinase (NIK), which itself is a central regulator for NFkB activation in response to TNF and IL-1. The functional IKK complex contains three subunits, IKK alpha, IKK beta and IKK gamma, and each appear to make essential contributions to IkB phosphorylation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ECI-6; I(Kappa)B(alpha); I-kappa-B-alpha; I-kappaBalpha; IkappaB alpha; IkappaBalpha; IkB-alpha; IKBalpha; Inhibitor of nuclear factor of kappa light chain gene enhancer in B-cells alpha; Inhibitor of nuclear factor of kappa light chain gene enhancer in B-cells, alpha; Major histocompatibility complex enhancer-binding protein MAD3; NF-kappa-B inhibitor alpha; nuclear factor of kappa light chain gene enhancer in B-cells; nuclear factor of kappa light chain gene enhancer in B-cells inhibitor, alpha; nuclear factor of kappa light polyp gene enhancer in B-cell 1; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha; RL/IF-1
Gene Aliases: AI462015; ECI-6/IKBA; IKBA; MAD-3; MAD3; NFKBI; NFKBIA; RL/IF-1
UniProt ID: (Pig) Q08353, (Human) P25963, (Rat) Q63746, (Mouse) Q9Z1E3
Entrez Gene ID: (Pig) 406188, (Dog) 480291, (Human) 4792, (Bovine) 282291, (Rat) 25493, (Mouse) 18035
protein import into nucleus
I-kappaB kinase/NF-kappaB signaling
cytoplasmic sequestering of NF-kappaB
negative regulation of macrophage derived foam cell differentiation
positive regulation of cholesterol efflux
negative regulation of lipid storage
lipopolysaccharide-mediated signaling pathway
positive regulation of cellular protein metabolic process
response to muramyl dipeptide
response to lipopolysaccharide
tumor necrosis factor-mediated signaling pathway
toll-like receptor 4 signaling pathway
response to muscle stretch
regulation of cell proliferation
cytoplasmic sequestering of transcription factor
response to exogenous dsRNA
negative regulation of myeloid cell differentiation
negative regulation of Notch signaling pathway
positive regulation of transcription from RNA polymerase II promoter
positive regulation of inflammatory response
nucleotide-binding oligomerization domain containing 1 signaling pathway
nucleotide-binding oligomerization domain containing 2 signaling pathway
protein import into nucleus, translocation
regulation of gene expression
negative regulation of NF-kappaB transcription factor activity
positive regulation of transcription, DNA-templated
stimulatory C-type lectin receptor signaling pathway
apoptotic process
viral process
Fc-epsilon receptor signaling pathway
regulation of NF-kappaB import into nucleus
negative regulation of apoptotic process
negative regulation of DNA binding
T cell receptor signaling pathway
positive regulation of NF-kappaB transcription factor activity
cellular response to cold
cellular response to cytokine stimulus
cellular response to organic cyclic compound
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support