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Invitrogen
IkB alpha Recombinant Rabbit Monoclonal Antibody (SZ00-07)
Advanced Verification
This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 10
IkB alpha Antibody (MA5-42418) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of of IKB alpha in SHG-44 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with IkB alpha Recombinant Monoclonal Antibody (Product # MA5-42418) at a dilution of 1:50 for 1 hour at room temperature, washed ... View More
Product Details
MA5-42418
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
SZ00-07
Immunogen
Synthetic peptide within human IKB alpha aa 1-50
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2911559
Product Specific Information
Positive Control: NIH/3T3 cell lysate, PC-12 cell lysate, Hela cell lysate, Hela, MCF-7, SHG-44, human kidney tissue, mouse kidney tissue, mouse brain tissue, mouse stomach tissue.
Subcellular Location: Cytoplasm, Nucleus.
Target Information
IkB-alpha is a 40 kDa protein that functions to inhibit NF- kappaB activity. The inhibition occurs via protein-protein interaction between I kappaB proteins and NF- kappaB dimers in the cytosol. The interaction of I kappa B-alpha with NF- kappaB masks the nuclear localization sequence of NF- kappaB, preventing NF- kappaB translocation to the nucleus. A variety of stimuli can activate gene expression by liberating NF- kappaB through the degradation of I kappaB alpha. These stimuli include the proinflammatory cytokines TNF- alpha and IL-1 beta, chemokines, PMA, growth factors, LPS, UV irradiation, viral infection, as well as various chemical and physical stresses. In humans, the gene is located on the q arm of chromosome 14. Activation of NFkB requires that IkB be phosphorylated on specific serine residues, which results in targeted degradation of IkB. IkB kinase alpha (IKK alpha), previously designated CHUK, interacts with IkB-alpha and specifically phosphorylates IkB-alpha on the sites that trigger its degradation Serines 32 and 36. IKK alpha appears to be critical for NFkB activation in response to proinflammatory cytokines. Phosphorylation of IkB by IKK alpha is stimulated by the NFkB inducing kinase (NIK), which itself is a central regulator for NFkB activation in response to TNF and IL-1. The functional IKK complex contains three subunits, IKK alpha, IKK beta and IKK gamma, and each appear to make essential contributions to IkB phosphorylation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: I(Kappa)B(alpha); I-kappa-B-alpha; I-kappaBalpha; IkappaBalpha; IkB-alpha; IKBalpha; Inhibitor of nuclear factor of kappa light chain gene enhancer in B-cells alpha; Inhibitor of nuclear factor of kappa light chain gene enhancer in B-cells, alpha; Major histocompatibility complex enhancer-binding protein MAD3; NF-kappa-B inhibitor alpha; nuclear factor of kappa light chain gene enhancer in B-cells; nuclear factor of kappa light chain gene enhancer in B-cells inhibitor, alpha; nuclear factor of kappa light polyp gene enhancer in B-cell 1; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha; RL/IF-1
Gene Aliases: AI462015; IKBA; MAD-3; MAD3; NFKBI; NFKBIA; RL/IF-1
UniProt ID: (Human) P25963, (Rat) Q63746, (Mouse) Q9Z1E3
Entrez Gene ID: (Human) 4792, (Rat) 25493, (Mouse) 18035
protein import into nucleus, translocation
cytoplasmic sequestering of NF-kappaB
regulation of gene expression
negative regulation of macrophage derived foam cell differentiation
positive regulation of cholesterol efflux
negative regulation of lipid storage
lipopolysaccharide-mediated signaling pathway
negative regulation of NF-kappaB transcription factor activity
positive regulation of cellular protein metabolic process
response to muramyl dipeptide
response to lipopolysaccharide
toll-like receptor 4 signaling pathway
response to muscle stretch
regulation of cell proliferation
cytoplasmic sequestering of transcription factor
response to exogenous dsRNA
negative regulation of myeloid cell differentiation
negative regulation of Notch signaling pathway
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
nucleotide-binding oligomerization domain containing 1 signaling pathway
nucleotide-binding oligomerization domain containing 2 signaling pathway
stimulatory C-type lectin receptor signaling pathway
apoptotic process
viral process
Fc-epsilon receptor signaling pathway
regulation of NF-kappaB import into nucleus
negative regulation of apoptotic process
negative regulation of DNA binding
T cell receptor signaling pathway
positive regulation of NF-kappaB transcription factor activity
cellular response to cold
cellular response to cytokine stimulus
cellular response to organic cyclic compound
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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