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Influenza A H1N1 NS Antibody (PA5-23364) in WB
Western blot analysis of Influenza A H1N1 NS in Influenza A H1N1 NS in a partial recombinant fusion protein containing amino acids 205-220. Samples were incubated in Influenza A H1N1 NS polyclonal antibody (Product # PA5-23364) using a dilution of 1 µg/mL followed by a goat anti-rabbit Ig HRP secondary antibody. PicoTect ECL substrate solution was used for this test.
Product Details
PA5-23364
Product Specifications
Species Reactivity
Virus
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Within the range of amino acids 205-239 of influenza A H1N1 NS
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2540887
Target Information
Influenza A H1N1 NS, also known as Non-structural protein 1 or NS1A or NS1, is a 234 amino acid containing protein belonging to the Influenza A virus (strain A/Swine/Iowa/15/1930 H1N1) which has aves, human and pig as host. This protein belongs to the influenza A viruses NS1 family and is a homodimer located in the nucleus of uninfected, transfected cells, and in the cytoplasm of infected cells. The viral protein NS1 binds to and inhibits two cellular proteins that are required for the 3'-end processing of cellular pre-mRNAs which include the 30 kDa cleavage and polyadenylation specificity factor (CPSF4) and the poly(A)-binding protein 2 (PABPN1). Thus inhibiting post-transcriptional processing of cellular pre-mRNA and leading to accumulation of unprocessed 3' end pre-mRNAs in nucleus and loss of cellular protein synthesis. This helps the virus to escape interferon synthesis after activation of the IRF3 pathway, and therefore prevents establishment of cellular antiviral state. NS1 is also known to prevent human EIF2AK2/PKR activation, at the very beginning of the infection, when NS1 is mainly present in the cytoplasm and prevent antiviral response.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: H1N1; H1N1 NS; Influenza H1N1
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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