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Influenza A M1 Antibody (PA5-32253) in WB
Western Blot using Influenza A M1 Polyclonal Antibody (Product # PA5-32253). Non-infected (–) and infected (+) C6/36 whole cell extracts (15 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Influenza A M1 Polyclonal Antibody Influenza A M1 Polyclonal Antibody (Product # PA5-32253) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Product Details
PA5-32253
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Virus
Published species
Virus
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein fragment of influenza A virus M1 (A/Puerto Rico/8/1934(H1N1))
Conjugate
Form
Liquid
Concentration
0.37 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol, 1% BSA
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2549726
Product Specific Information
Recommended positive controls: DF-1, WSN virus infected DF-1.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Influenza A virus matrix 1, M1, is a critical protein required for assembly and budding. Hemagglutinin associates with M1 via its cytoplasmic tail and transmembrane domain. The M2 and nucleoprotein proteins are critical in the replication cycle of influenza viruses. The M2 channel protein is an essential component of the viral envelope because of its ability to form a highly selective, pH-regulated, proton-conducting channel. The M2 channel allows protons to enter the interior of the virus and acidification weakens the interaction of the M1 protein with the ribonuclear core.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Influenza M1
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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