Invitrogen

Influenza A M2 Monoclonal Antibody (14C2)

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This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

9 Published Figures

15 References

View all (5) Influenza A M2 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Influenza A M2 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 11

Influenza A M2 Antibody (MA1-082) in ICC/IF

Immunofluorescence staining of infected MDCK cells using Product # MA1-082

Influenza A M2 Antibody in Western Blot (WB)

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Product Details

MA1-082

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 12 publications 12 publications

Immunocytochemistry (ICC/IF)

1 µg/mL

View 2 publications 2 publications

Flow Cytometry (Flow)

Assay-dependent

View 1 publication 1 publication

Immunoprecipitation (IP)

10 µg/mL

Product Specifications

Species Reactivity

Virus

Published species

Virus

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

14C2

Immunogen

M2 protein from A/WSN/33-infected CV1 cell lysate.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_325043

Product Specific Information

MA1-082 detects the N-terminal of the influenza A virus M2 protein.

MA1-082 has been successfully used in Western blot, Immunofluorescence, immunocytochemistry and immunoprecipitation procedures. By Western blot, this antibody detects a ~15 kDa protein representing the M2 protein. MA1-082 has demonstrated the ability to immunoprecipitate M2 from [35s] cysteine-labeled WSN-infected cells.

MA1-082 has demonstrated the ability to restrict the replication of some strains of influenza A virus by binding to the extracellular N-Terminal domain.

The MA1-082 immunogen is M2 protein from A/WSN/33-infected CV1 cell lysate.

Target Information

Influenza A virus is an enveloped virus encoding 10 polypeptides. RNA segment 7 encodes for two proteins: M1 (matrix 1) and M2 (matrix 2). M1 protein is encoded by an mRNA that is colinear, while M2 protein is synthesized from spliced mRNA. M2 protein is a transmembrane protein composed of three Domains: 1) 24 residues representing the N-terminal region, 2) 19 hydro-phobic residues that serve as a membrane anchor, and 3) 54 residues near the C-terminal in the cytoplasmic domain. The M2 protein has been found to play a role in Influenza replication and assembly of virion particles. Further experimentation has demonstrated that this protein is an acid-activated ion channel for virus replication.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The Hypothiocyanite and Amantadine Combination Treatment Prevents Lethal Influenza A Virus Infection in Mice.

Frontiers in immunology

Ashtiwi NM,Sarr D,Nagy T,Reneer ZB,Tripp RA,Rada B

Published figure using Influenza A M2 monoclonal antibody (Product # MA1-082) in Immunocytochemistry

Wed Jun 08 00:00:00 EDT 2022

Nuclear speckle integrity and function require TAO2 kinase.

Proceedings of the National Academy of Sciences of the United States of America

Gao S,Esparza M,Dehghan I,Aksenova V,Zhang K,Batten K,Ferretti MB,Begg BE,Cagatay T,Shay JW,García-Sastre A,Goldsmith EJ,Chen ZJ,Dasso M,Lynch KW,Cobb MH,Fontoura BMA

MA1-082 was used in Western Blotting to find that a nuclear pool of TAO2 is localized at nuclear speckles and interacts with nuclear speckle factors involved in RNA splicing and nuclear export, including SRSF1 and Aly/Ref.

Tue Jun 21 00:00:00 EDT 2022

Expression of influenza A virus-derived peptides on a rotavirus VP6-based delivery platform.

Archives of virology

Gröhn S,Heinimäki S,Tamminen K,Blazevic V

MA1-082 was used in Western Blot to explore different rotavirus medium-layer VP6 protein insertion sites for the presentation of two influenza A virus peptides toward potential vaccination strategies.

Fri Jan 01 00:00:00 EST 2021

Thapsigargin Is a Broad-Spectrum Inhibitor of Major Human Respiratory Viruses: Coronavirus, Respiratory Syncytial Virus and Influenza A Virus.

Viruses

Al-Beltagi S,Preda CA,Goulding LV,James J,Pu J,Skinner P,Jiang Z,Wang BL,Yang J,Banyard AC,Mellits KH,Gershkovich P,Hayes CJ,Nguyen-Van-Tam J,Brown IH,Liu J,Chang KC

Published figure using Influenza A M2 monoclonal antibody (Product # MA1-082) in Western Blot

Wed Feb 03 00:00:00 EST 2021

Thapsigargin Is a Broad-Spectrum Inhibitor of Major Human Respiratory Viruses: Coronavirus, Respiratory Syncytial Virus and Influenza A Virus.

Viruses

Al-Beltagi S,Preda CA,Goulding LV,James J,Pu J,Skinner P,Jiang Z,Wang BL,Yang J,Banyard AC,Mellits KH,Gershkovich P,Hayes CJ,Nguyen-Van-Tam J,Brown IH,Liu J,Chang KC

Published figure using Influenza A M2 monoclonal antibody (Product # MA1-082) in Western Blot

Wed Feb 03 00:00:00 EST 2021

Influenza A virus co-opts ERI1 exonuclease bound to histone mRNA to promote viral transcription.

Nucleic acids research

Declercq M,Biquand E,Karim M,Pietrosemoli N,Jacob Y,Demeret C,Barbezange C,van der Werf S

MA1-082 was used in Western Blotting to provide evidence that during infection the SLBP protein and histone mRNAs co-purify with vRNPs alongside ERI1.

Fri Oct 09 00:00:00 EDT 2020

Influenza A virus co-opts ERI1 exonuclease bound to histone mRNA to promote viral transcription.

Nucleic acids research

Declercq M,Biquand E,Karim M,Pietrosemoli N,Jacob Y,Demeret C,Barbezange C,van der Werf S

MA1-082 was used in Western Blotting to provide evidence that during infection the SLBP protein and histone mRNAs co-purify with vRNPs alongside ERI1.

Fri Oct 09 00:00:00 EDT 2020

Double-Layered M2e-NA Protein Nanoparticle Immunization Induces Broad Cross-Protection against Different Influenza Viruses in Mice.

Advanced healthcare materials

Wang Y,Deng L,Gonzalez GX,Luthra L,Dong C,Ma Y,Zou J,Kang SM,Wang BZ

MA1-082 was used in Western Blotting to investigate if double-layered protein nanoparticles containing structure-stabilized M2e and NA can be developed into a universal influenza vaccine or a synergistic component of such vaccines.

Wed Jan 01 00:00:00 EST 2020

Chemical intervention of influenza virus mRNA nuclear export.

PLoS pathogens

Esparza M,Mor A,Niederstrasser H,White K,White A,Zhang K,Gao S,Wang J,Liang J,Sho S,Sakthivel R,Sathe AA,Xing C,Muñoz-Moreno R,Shay JW,García-Sastre A,Ready J,Posner B,Fontoura BMA

MA1-082 was used in Western Blot to identify inhibitors of influenza A viral mRNA biogenesis and nuclear export that exhibit no significant activity towards bulk cellular mRNA at non-cytotoxic concentrations.

Wed Apr 01 00:00:00 EDT 2020

A non-death function of the mitochondrial apoptosis apparatus in immunity.

The EMBO journal

Brokatzky D,Dörflinger B,Haimovici A,Weber A,Kirschnek S,Vier J,Metz A,Henschel J,Steinfeldt T,Gentle IE,Häcker G

MA1-082 was used in Immunocytochemistry-immunoflourescence to report that such sub-lethal engagement of mitochondrial apoptosis signaling causes the secretion of cytokines from human epithelial cells in a process controlled by the Bcl-2 family of proteins. As well as showing that sub-lethal signaling of the mitochondrial apoptosis pathway is initiated by infections with all tested viral, bacterial, and protozoan pathogens and causes damage to the genomic DNA.

Mon Jun 03 00:00:00 EDT 2019

Double-layered protein nanoparticles induce broad protection against divergent influenza A viruses.

Nature communications

Deng L,Mohan T,Chang TZ,Gonzalez GX,Wang Y,Kwon YM,Kang SM,Compans RW,Champion JA,Wang BZ

MA1-082 was used in Western Blotting to report a methodology to generate double-layered protein nanoparticles as a universal influenza vaccine.

Wed Jan 24 00:00:00 EST 2018

Influenza virus differentially activates mTORC1 and mTORC2 signaling to maximize late stage replication.

PLoS pathogens

Kuss-Duerkop SK,Wang J,Mena I,White K,Metreveli G,Sakthivel R,Mata MA,Muñoz-Moreno R,Chen X,Krammer F,Diamond MS,Chen ZJ,García-Sastre A,Fontoura BMA

Published figure using Influenza A M2 monoclonal antibody (Product # MA1-082) in Western Blot

Fri Sep 01 00:00:00 EDT 2017

Recruitment of RED-SMU1 complex by Influenza A Virus RNA polymerase to control Viral mRNA splicing.

PLoS pathogens

Fournier G,Chiang C,Munier S,Tomoiu A,Demeret C,Vidalain PO,Jacob Y,Naffakh N

MA1-082 was used in western blot to determine viral mRNA splicing controlled by the recruitment of RED-SMU1 complex by Influenza A Virus RNA polymerase

Sun Jun 01 00:00:00 EDT 2014

Kinetics of proton transport into influenza virions by the viral M2 channel.

PloS one

Ivanovic T,Rozendaal R,Floyd DL,Popovic M,van Oijen AM,Harrison SC

MA1-082 was used in Western Blotting to study the kinetics of proton transport into influenza virions by the viral M2 channel.

Thu Aug 02 00:00:00 EDT 2012

Increased immunogenicity and protective efficacy of influenza M2e fused to a tetramerizing protein.

PloS one

Andersson AM,Håkansson KO,Jensen BA,Christensen D,Andersen P,Thomsen AR,Christensen JP

MA1-082 was used in flow cytometry to generate a tetrameric form of the influenza A M2e protein ectodomain with increased immunogenicity and protective efficacy

Fri Feb 22 00:00:00 EST 2013

Characterization of a fully human monoclonal antibody against extracellular domain of matrix protein 2 of influenza A virus.

Antiviral research

Ozawa T,Jin A,Tajiri K,Takemoto M,Okuda T,Shiraki K,Kishi H,Muraguchi A

MA1-082 was used in western blot to isolate and characterize a human antibody to influenza virus matrix protein 2 (M2e)

Thu Sep 01 00:00:00 EDT 2011

Matrix protein 2 of influenza A virus blocks autophagosome fusion with lysosomes.

Cell host & microbe

Gannagé M,Dormann D,Albrecht R,Dengjel J,Torossi T,Rämer PC,Lee M,Strowig T,Arrey F,Conenello G,Pypaert M,Andersen J,García-Sastre A,Münz C

MA1-082 was used in western blot to study the role of matrix protein 2 of influenza A virus in blocking autophagosome fusion with lysosomes

Thu Oct 22 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: Influenza M2

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Influenza A M2 Monoclonal Antibody (14C2)

Influenza A M2 Antibody (MA1-082) in ICC/IF

Product Details

MA1-082

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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