Invitrogen

Influenza A NP Polyclonal Antibody

Influenza A NP Antibody in Immunocytochemistry (ICC/IF)

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Influenza A NP Antibody (PA5-32242) in ICC/IF

Influenza A Virus NP antibody detects NP (H1N1) protein at by immunofluorescent analysis. Sample: A/WSN/33 infected Vero cells were fixed in 4% paraformaldehyde at RT for 20 min. Green: NP (H1N1) protein stained by Influenza A Virus NP antibody (Product # PA5-32242) diluted at 1:500. Blue: DAPI staining. Yellow: WGA life stained at 37°C,30 min.

Influenza A NP Antibody in Western Blot (WB)

Influenza A NP Antibody in ELISA (ELISA)

Influenza A NP Antibody in Immunohistochemistry (IHC)

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Product Details

PA5-32242

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000-1:20,000

View 10 publications 10 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:1,000

View 4 publications 4 publications

ELISA (ELISA)

Assay-dependent

View 1 publication 1 publication

Product Specifications

Species Reactivity

Virus

Published species

Virus

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein fragment of Influenza A virus NP (A/WSN/1933(H1N1))

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 1% BSA, 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2549715

Product Specific Information

Recommended positive controls: H1N1-infected H1299, DF1 (Influenza A virus treatment for 10hr), influenza virus (A/WSN/33) infected MDCK.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

The nucleoprotein (NP) of Influenza A virus encapsidates the negative strand of the viral RNA and is essential for replicative transcription. It may also be involved in other essential functions throughout the virus life cycle. As well as binding ssRNA, NP is able to self associate to form large oligomeric complexes. NP is able to interact with a variety of other macromolecules of both viral and cellular origins. It binds the PB1 and PB2 subunits of the polymerase and the matrix protein M1. "NP has also been shown to interact with at least four cellular polypeptide families: nuclear import receptors of the importin class, filamentous (F) actin, the nuclear export receptor CRM1 and a DEAD box helicase BAT1/UAP56" (Portela et al 2002).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

NRF2 activators inhibit influenza A virus replication by interfering with nucleo-cytoplasmic export of viral RNPs in an NRF2-independent manner.

PLoS pathogens

Waqas FH,Shehata M,Elgaher WAM,Lacour A,Kurmasheva N,Begnini F,Kiib AE,Dahlmann J,Chen C,Pavlou A,Poulsen TB,Merkert S,Martin U,Olmer R,Olagnier D,Hirsch AKH,Pleschka S,Pessler F

PA5-32242 was used in Western Blotting to evaluate the ability of the NRF2 activators 4-octyl itaconate (4OI), bardoxolone methyl (BARD), sulforaphane (SFN), and the inhibitor of exportin-1 (XPO1)-mediated nuclear export selinexor (SEL) to interfere with influenza virus A/Puerto Rico/8/1934 (H1N1) infection of human cells.

Sat Jul 01 00:00:00 EDT 2023

Nonclassical autophagy activation pathways are essential for production of infectious Influenza A virus in vitro.

Molecular microbiology

Bell TAS,Velappan N,Gleasner CD,Xie G,Starkenburg SR,Waldo G,Banerjee S,Micheva-Viteva SN

Published figure using Influenza A NP polyclonal antibody (Product # PA5-32242) in Western Blot

Tue Feb 01 00:00:00 EST 2022

The IFN-inducible GTPase IRGB10 regulates viral replication and inflammasome activation during influenza A virus infection in mice.

European journal of immunology

Christgen S,Place DE,Zheng M,Briard B,Yamamoto M,Kanneganti TD

PA5-32242 was used in ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to demonstrate a detrimental role of IRGB10 in host immunity in response to IAV and a novel function of IRGB10, but not IRGMs, in promoting viral translocation into the nucleus.

Tue Feb 01 00:00:00 EST 2022

Generalized Modules for Membrane Antigens (GMMA), an outer membrane vesicle-based vaccine platform, for efficient viral antigen delivery.

Journal of extracellular vesicles

Hu K,Palmieri E,Samnuan K,Ricchetti B,Oldrini D,McKay PF,Wu G,Thorne L,Fooks AR,McElhinney LM,Goharriz H,Golding M,Shattock RJ,Micoli F

PA5-32242 was used in Enzyme-linked immunosorbent assay to evaluate Generalized Modules for Membrane Antigens (GMMA), outer membrane vesicles (OMVs) generated by genetically modified Gram-negative bacteria, as a vaccine platform for viral pathogens.

Tue Nov 01 00:00:00 EDT 2022

DDX3X coordinates host defense against influenza virus by activating the NLRP3 inflammasome and type I interferon response.

The Journal of biological chemistry

Kesavardhana S,Samir P,Zheng M,Malireddi RKS,Karki R,Sharma BR,Place DE,Briard B,Vogel P,Kanneganti TD

Published figure using Influenza A NP polyclonal antibody (Product # PA5-32242) in Western Blot

Wed Aug 18 00:00:00 EDT 2021

DDX3X coordinates host defense against influenza virus by activating the NLRP3 inflammasome and type I interferon response.

The Journal of biological chemistry

Kesavardhana S,Samir P,Zheng M,Malireddi RKS,Karki R,Sharma BR,Place DE,Briard B,Vogel P,Kanneganti TD

Published figure using Influenza A NP polyclonal antibody (Product # PA5-32242) in Western Blot

Wed Aug 18 00:00:00 EDT 2021

Influenza A viruses limit NLRP3-NEK7-complex formation and pyroptosis in human macrophages.

EMBO reports

Boal-Carvalho I,Mazel-Sanchez B,Silva F,Garnier L,Yildiz S,Bonifacio JP,Niu C,Williams N,Francois P,Schwerk N,Schöning J,Carlens J,Viemann D,Hugues S,Schmolke M

PA5-32242 was used in Western Blot to show that interaction of a recently identified licensing kinase NEK7 with NLRP3 is diminished, which is required to initiate inflammasome assembly.

Thu Dec 03 00:00:00 EST 2020

The Pyrimidine Analog FNC Potently Inhibits the Replication of Multiple Enteroviruses.

Journal of virology

Xu N,Yang J,Zheng B,Zhang Y,Cao Y,Huan C,Wang S,Chang J,Zhang W

PA5-32242 was used in Western Blotting to demonstrate that FNC (2''-deoxy-2''-β-fluoro-4''-azidocytidine) potently inhibits the viral replication of a multitude of EVs, including enterovirus 71 (EV71), coxsackievirus A16 (CA16), CA6, EVD68, and coxsackievirus B3 (CVB3), at the nanomolar level.

Thu Apr 16 00:00:00 EDT 2020

Enhancing Neuraminidase Immunogenicity of Influenza A Viruses by Rewiring RNA Packaging Signals.

Journal of virology

Zheng A,Sun W,Xiong X,Freyn AW,Peukes J,Strohmeier S,Nachbagauer R,Briggs JAG,Krammer F,Palese P

PA5-32242 was used in Western Blotting to demonstrate that by swapping the 5' and 3' terminal packaging signals of the HA and NA genomic segments, which contain the RNA promoters, influenza viruses can be rescued to express more NA and less HA.

Thu Jul 30 00:00:00 EDT 2020

Structure and Sequence Determinants Governing the Interactions of RNAs with Influenza A Virus Non-Structural Protein NS1.

Viruses

Wacquiez A,Coste F,Kut E,Gaudon V,Trapp S,Castaing B,Marc D

PA5-32242 was used in Western Blotting to recognise structural and sequence determinants that allow interaction between RNA and influenza A virus non-structural protein NS1.

Thu Aug 27 00:00:00 EDT 2020

ANP32B Deficiency Protects Mice From Lethal Influenza A Virus Challenge by Dampening the Host Immune Response.

Frontiers in immunology

Beck S,Zickler M,Pinho Dos Reis V,Günther T,Grundhoff A,Reilly PT,Mak TW,Stanelle-Bertram S,Gabriel G

PA5-32242 was used in Immunohistochemistry to show that ANP32B but not ANP32A promotes IAV pathogenesis in mice.

Fri Mar 19 00:00:00 EDT 2021

Testosterone Protects Against Severe Influenza by Reducing the Pro-Inflammatory Cytokine Response in the Murine Lung.

Frontiers in immunology

Tuku B,Stanelle-Bertram S,Sellau J,Beck S,Bai T,Kouassi NM,Preuß A,Hoenow S,Renné T,Lotter H,Gabriel G

PA5-32242 was used in Immunohistochemistry to show that, in mice, testosterone has protective functions from the 2009 H1N1 influenza A virus infection, as it reduces lung damage by reducing of IL-1β cytokine expression levels.

Wed Mar 31 00:00:00 EDT 2021

Thapsigargin at Non-Cytotoxic Levels Induces a Potent Host Antiviral Response that Blocks Influenza A Virus Replication.

Viruses

Goulding LV,Yang J,Jiang Z,Zhang H,Lea D,Emes RD,Dottorini T,Pu J,Liu J,Chang KC

PA5-32242 was used in Western Blotting to suggest that TG is potentially a viable host-centric antiviral for the treatment of influenza A virus infection without the inherent problem of drug resistance.

Sun Sep 27 00:00:00 EDT 2020

Thapsigargin at Non-Cytotoxic Levels Induces a Potent Host Antiviral Response that Blocks Influenza A Virus Replication.

Viruses

Goulding LV,Yang J,Jiang Z,Zhang H,Lea D,Emes RD,Dottorini T,Pu J,Liu J,Chang KC

Sun Sep 27 00:00:00 EDT 2020

Influenza A Virus Utilizes Low-Affinity, High-Avidity Interactions with the Nuclear Import Machinery To Ensure Infection and Immune Evasion.

Journal of virology

Tome-Amat J,Ramos I,Amanor F,Fernández-Sesma A,Ashour J

PA5-32242 was used in Western Blotting to show that genomes that become frustrated by low avidity and remain cytoplasmic trigger the type I interferon response.

Tue Jan 01 00:00:00 EST 2019

Immunodominance of Antigenic Site B in the Hemagglutinin of the Current H3N2 Influenza Virus in Humans and Mice.

Journal of virology

Broecker F,Liu STH,Sun W,Krammer F,Simon V,Palese P

PA5-32242 was used in Immunocytochemistry to reveal that antigenic site B is immunodominant in the H3N2 influenza virus strain included in the current vaccine preparations.

Mon Oct 15 00:00:00 EDT 2018

Bat lung epithelial cells show greater host species-specific innate resistance than MDCK cells to human and avian influenza viruses.

Virology journal

Slater T,Eckerle I,Chang KC

PA5-32242 was used in Western Blot to determine the extent bats are susceptible to conventional avian and human influenza A viruses.

Tue Apr 10 00:00:00 EDT 2018

A conserved influenza A virus nucleoprotein code controls specific viral genome packaging.

Nature communications

Moreira ÉA,Weber A,Bolte H,Kolesnikova L,Giese S,Lakdawala S,Beer M,Zimmer G,García-Sastre A,Schwemmle M,Juozapaitis M

PA532242 was used in immunocytochemistry and western blot to report that distinct amino acids in the viral nucleoprotein are required for packaging specific influenza A RNA segments

Wed Sep 21 00:00:00 EDT 2016

Differential replication properties among H9N2 avian influenza viruses of Eurasian origin.

Veterinary research

Parvin R,Shehata AA,Heenemann K,Gac M,Rueckner A,Halami MY,Vahlenkamp TW

Published figure using Influenza A NP polyclonal antibody (Product # PA5-32242) in Immunofluorescence

Mon Jul 06 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: Influenza nucleoprotein

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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Influenza A NP Polyclonal Antibody

Influenza A NP Antibody (PA5-32242) in ICC/IF

Product Details

PA5-32242

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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