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Invitrogen
Influenza A NP Monoclonal Antibody (D67J), FITC
FIGURE: 1 / 2
Influenza A NP Antibody (MA1-7322) in Flow
293T cells were infected with influenza PR8 virus (moi 0.001) for 24hours. Cells were harvested for FACS analysis. Anti-Influenza A NP Monoclonal Antibody, FITC Conjugate (Product # MA1-7322) was used at dilutions of 1:10, 1:20 and 1:40 to stain for influenza NP in these samples. Data courtesy of the Innovators Program.
Product Details
MA1-7322
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Virus
Published species
Virus
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
D67J
Immunogen
Influenza A Nucleoprotein.
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
0.1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.2, with 10mg/mL BSA
Contains
0.1% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_1017747
Product Specific Information
The MA1-7322 antibody reacts with Influenza A virus NP in viral and infected tissue samples. Reactive with all A strains.
MA1-7322 has been successfully used in immunofluorescence, FACS, and ELISA applications. The recommended starting dilution is 1:5 - 1:20 but optimal dilution should be determined by the end user. Acetone fixation of the antigen source is recommended prior to staining.
The MA1-7322 antibody was raised against Influenza A Nucleoprotein.
Target Information
The nucleoprotein (NP) of Influenza A virus encapsidates the negative strand of the viral RNA and is essential for replicative transcription. It may also be involved in other essential functions throughout the virus life cycle. As well as binding ssRNA, NP is able to self associate to form large oligomeric complexes. NP is able to interact with a variety of other macromolecules of both viral and cellular origins. It binds the PB1 and PB2 subunits of the polymerase and the matrix protein M1. "NP has also been shown to interact with at least four cellular polypeptide families: nuclear import receptors of the importin class, filamentous (F) actin, the nuclear export receptor CRM1 and a DEAD box helicase BAT1/UAP56" (Portela et al 2002).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Influenza nucleoprotein
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