Invitrogen

Influenza A NS1 Polyclonal Antibody

Influenza A NS1 Antibody in Immunocytochemistry (ICC/IF)

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Influenza A NS1 Antibody (PA5-32243) in ICC/IF

Influenza A Virus NS1 antibody detects NS1 (H1N1) protein at by immunofluorescent analysis. Sample: A/WSN/33 infected Vero cells were fixed in 4% paraformaldehyde at RT for 20 min. Green: NS1 (H1N1) protein stained by Influenza A Virus NS1 antibody (Product # PA5-32243) diluted at 1:500. Blue: DAPI staining. Yellow: WGA life stained at 37°C,30 min.

Influenza A NS1 Antibody in Western Blot (WB)

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Product Details

PA5-32243

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

Immunocytochemistry (ICC/IF)

1:100-1:1,000

View 1 publication 1 publication

Product Specifications

Species Reactivity

Virus

Published species

Virus

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein fragment of Influenza A virus NS1 (A/WSN/1933(H1N1))

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.36 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2549716

Product Specific Information

Recommended positive controls: Influenza virus (A/WSN/1933(H1N1))-infected DF-1 cell lysates, Influenza virus-infected monkey vero cells.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Influenza A viruses are single stranded, negative sense RNA viruses. Two distinct non-structural proteins NS1 and NS2 are translated from one of the eight RNA segments by using different reading frames.The influenza A virus NS2 is located in the nucleus and the cytoplasm. NS2 is essential for nuclear export of influenza virus ribonucleoprotein (RNP) complexes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Influenza A Virus Utilizes Low-Affinity, High-Avidity Interactions with the Nuclear Import Machinery To Ensure Infection and Immune Evasion.

Journal of virology

Tome-Amat J,Ramos I,Amanor F,Fernández-Sesma A,Ashour J

PA5-32243 was used in Western Blotting to show that genomes that become frustrated by low avidity and remain cytoplasmic trigger the type I interferon response.

Tue Jan 01 00:00:00 EST 2019

Nuclear-resident RIG-I senses viral replication inducing antiviral immunity.

Nature communications

Liu G,Lu Y,Thulasi Raman SN,Xu F,Wu Q,Li Z,Brownlie R,Liu Q,Zhou Y

PA5-32243 was used in Western Blotting to report the identification of nuclear-resident RIG-I actively involved in nuclear viral RNA sensing.

Fri Aug 10 00:00:00 EDT 2018

Human nasal epithelial cells derived from multiple subjects exhibit differential responses to H3N2 influenza virus infection in vitro.

The Journal of allergy and clinical immunology

Yan Y,Tan KS,Li C,Tran T,Chao SS,Sugrue RJ,Shi L,Chow VT,Wang DY

PA5-32243 was used in immunocytochemistry to evaluate the differential responses to H3N2 influenza virus infection in vitro in human nasal epithelial cells derived from multiple subjects

Fri Jul 01 00:00:00 EDT 2016

Inhibition of influenza A virus matrix and nonstructural gene expression using RNA interference.

Virology

McMillen CM,Beezhold DH,Blachere FM,Othumpangat S,Kashon ML,Noti JD

PA532243 was used in western blot to evaluate the use of small interfering RNA molecules as an anti-influenza virus therapy

Sat Oct 01 00:00:00 EDT 2016

Infection of Mouse Macrophages by Seasonal Influenza Viruses Can Be Restricted at the Level of Virus Entry and at a Late Stage in the Virus Life Cycle.