Invitrogen

Influenza A PB1 Polyclonal Antibody

Influenza A PB1 Antibody in Immunocytochemistry (ICC/IF)

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Influenza A PB1 Antibody (PA5-34914) in ICC/IF

Influenza A Virus PB1 antibody detects PB1 protein at by immunofluorescent analysis. Sample: A/WSN/33 infected Vero cells were fixed in 4% paraformaldehyde at RT for 20 min. Green: PB1 protein stained by Influenza A Virus PB1 antibody (Product # PA5-34914) diluted at 1:500. Blue: DAPI staining. Yellow: WGA life stained at 37°C,30 min.

Influenza A PB1 Antibody in Western Blot (WB)

Influenza A PB1 Antibody in CUT&RUN (C&R)

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Product Details

PA5-34914

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 4 publications 4 publications

Immunocytochemistry (ICC/IF)

1:100-1:1,000

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

CUT&RUN (C&R)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Virus

Published species

Virus

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to N-terminal of Influenza A virus PB1 (A/Puerto Rico/8/1934(H1N1))

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol, 1% BSA

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2552264

Product Specific Information

Recommended positive controls: WSN virus infected DF1 10hr.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Influenza A virus contains eight single stranded, negative sense RNAs. Each RNA is associated with viral RNA-dependent RNA polymerase which consists of PA, PB1 and PB2 proteins. PB1 is the subunit involved in the catalytic activity of nucleotide polymerization and is involved in the initiation of transcription. Both PB1 and PB2 can be crosslinked to synthetic RNA with the 3' terminal sequence of viral RNA. PB1 and PB2 may also be involved in recognition of the promoter and/or replication origin on template viral RNA. PB2 is the cap 1-recognition protein, which binds to the cap structure of host mRNA. The cap structure then acts as a primer for transcription to produce viral mRNA.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A universal influenza mRNA vaccine candidate boosts T cell responses and reduces zoonotic influenza virus disease in ferrets.

Science advances

van de Ven K,Lanfermeijer J,van Dijken H,Muramatsu H,Vilas Boas de Melo C,Lenz S,Peters F,Beattie MB,Lin PJC,Ferreira JA,van den Brand J,van Baarle D,Pardi N,de Jonge J

PA5-34914 was used in Western Blotting to show that mRNA vaccines encoding internal influenza virus proteins represent a promising strategy to induce broadly protective T cell immunity against influenza viruses.

Wed Dec 14 00:00:00 EST 2022

Cell type- and replication stage-specific influenza virus responses in vivo.

PLoS pathogens

Fay EJ,Aron SL,Macchietto MG,Markman MW,Esser-Nobis K,Gale M,Shen S,Langlois RA

PA5-34914 was used in Western Blotting to characterize the early immune response to IAV in vitro and in vivo.

Sat Aug 01 00:00:00 EDT 2020

CUT&RUN detects distinct DNA footprints of RNA polymerase II near the transcription start sites.

Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology

Miura M,Chen H

PA5-34914 was used in CUT&RUN sequencing to show that the positioning of the large-size-class DNA footprints around the short-fragment peak was associated with the directionality of transcription, demonstrating the biological significance of distinct CUT&RUN footprints of RNA polymerase II.

Tue Dec 01 00:00:00 EST 2020

CUT&RUN detects distinct DNA footprints of RNA polymerase II near the transcription start sites.

Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology

Miura M,Chen H

Tue Dec 01 00:00:00 EST 2020

CUT&RUN detects distinct DNA footprints of RNA polymerase II near the transcription start sites.

Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology

Miura M,Chen H

Tue Dec 01 00:00:00 EST 2020

Influenza virus polymerase subunits co-evolve to ensure proper levels of dimerization of the heterotrimer.

PLoS pathogens

Chen KY,Santos Afonso ED,Enouf V,Isel C,Naffakh N

PA5-34914 was used in Western Blot to point to influenza polymerase dimerization as a feature that is controlled by a complex interplay of genetic determinants, can restrict genetic reassortment, and could become a target for antiviral drug development.

Tue Oct 01 00:00:00 EDT 2019

Species specific differences in use of ANP32 proteins by influenza A virus.

eLife

Long JS,Idoko-Akoh A,Mistry B,Goldhill D,Staller E,Schreyer J,Ross C,Goodbourn S,Shelton H,Skinner MA,Sang H,McGrew MJ,Barclay W

PA5-34914 was used in Western Blotting to understand ANP32 function to help develop antiviral strategies and aid the design of influenza virus resilient genome edited chickens.

Tue Jun 04 00:00:00 EDT 2019

Bioinformatics

Protein Aliases: Influenza PB1

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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Influenza A PB1 Polyclonal Antibody

Influenza A PB1 Antibody (PA5-34914) in ICC/IF

Product Details

PA5-34914

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Miscellaneous PubMed (Misc)

CUT&RUN (C&R)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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