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Influenza A PB2 Antibody (PA5-32221) in ICC/IF
Influenza A Virus PB2 antibody detects PB2 protein at by immunofluorescent analysis. Sample: A/WSN/33 infected Vero cells were fixed in 4% paraformaldehyde at RT for 20 min. Green: PB2 protein stained by Influenza A Virus PB2 antibody (Product # PA5-32221) diluted at 1:500. Blue: DAPI staining. Yellow: WGA life stained at 37°C,30 min.
Product Details
PA5-32221
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Virus
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein fragment of influenza A virus PB2 (A/Puerto Rico/8/1934(H1N1))
Conjugate
Form
Liquid
Concentration
1.27 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2549694
Product Specific Information
Recommended positive controls: WSN virus infected DF1, H1N1-infected H1299.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Influenza A virus contains eight single stranded, negative sense RNAs. Each RNA is associated with viral RNA-dependent RNA polymerase which consists of PA, PB1 and PB2 proteins. PB1 is the subunit involved in the catalytic activity of nucleotide polymerization and is involved in the initiation of transcription. Both PB1 and PB2 can be crosslinked to synthetic RNA with the 3' terminal sequence of viral RNA. PB1 and PB2 may also be involved in recognition of the promoter and/or replication origin on template viral RNA. PB2 is the cap 1-recognition protein, which binds to the cap structure of host mRNA. The cap structure then acts as a primer for transcription to produce viral mRNA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Influenza PB2
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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