Rockland

Insulin Monoclonal Antibody (2D11.H5)

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

200-301-389

Applications
Tested Dilution
Publications

Western Blot (WB)

1:50-1:200
-

Immunohistochemistry (IHC)

1:50-1:200
-

ELISA (ELISA)

1:5,000-1:25,000
-

Immunoprecipitation (IP)

1:50-1:200
-

Dot blot (DB)

1:400
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

2D11.H5

Immunogen

This protein A purified monoclonal antibody was produced by repeated immunizations with purified human insulin coupled to bovine serum albumin (BSA).
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Reacts specifically with insulin from human and swine sources. Cross reactivity with insulin from mouse and rat does not occur. Cross reactivity with insulin from other sources has not been determined.

Target Information

Insulin is a peptide hormone secreted by beta cells of the pancreatic islets. It regulates carbohydrate, protein and lipid metabolism by enhancing membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Insulin is 51-amino acid polypeptide product produced from a precursor peptide, proinsulin. Proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds and one molecule of C-peptide. Insulin deficiency results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of b-cell origin such as insulinoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Ins; Insulin; preproinsulin; proinsulin

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Gene Aliases: IDDM; IDDM1; IDDM2; ILPR; INS; IRDN; MODY10

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UniProt ID: (Human) P01308

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Entrez Gene ID: (Human) 3630

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Function(s)
protease binding insulin receptor binding insulin-like growth factor receptor binding hormone activity protein binding identical protein binding growth factor
Process(es)
MAPK cascade negative regulation of acute inflammatory response glucose metabolic process regulation of transcription, DNA-templated regulation of cellular amino acid metabolic process ER to Golgi vesicle-mediated transport acute-phase response G-protein coupled receptor signaling pathway cell-cell signaling positive regulation of cell proliferation insulin receptor signaling pathway positive regulation of gene expression positive regulation of phosphatidylinositol 3-kinase signaling glucose transport regulation of transmembrane transporter activity positive regulation of cell growth positive regulation of cell migration positive regulation of protein autophosphorylation activation of protein kinase B activity positive regulation of cellular protein metabolic process negative regulation of protein oligomerization regulation of protein localization negative regulation of NAD(P)H oxidase activity wound healing negative regulation of protein catabolic process glucose homeostasis positive regulation of MAPK cascade cellular protein metabolic process positive regulation of nitric oxide biosynthetic process positive regulation of cell differentiation negative regulation of gluconeogenesis positive regulation of glycogen biosynthetic process positive regulation of DNA replication negative regulation of glycogen catabolic process positive regulation of glycolytic process positive regulation of mitotic nuclear division negative regulation of proteolysis negative regulation of vasodilation positive regulation of vasodilation negative regulation of fatty acid metabolic process positive regulation of glucose import positive regulation of insulin receptor signaling pathway alpha-beta T cell activation positive regulation of lipid biosynthetic process regulation of protein secretion negative regulation of protein secretion positive regulation of cytokine secretion positive regulation of peptidyl-tyrosine phosphorylation negative regulation of lipid catabolic process positive regulation of nitric-oxide synthase activity positive regulation of NF-kappaB transcription factor activity positive regulation of protein kinase B signaling fatty acid homeostasis negative regulation of respiratory burst involved in inflammatory response positive regulation of respiratory burst positive regulation of peptide hormone secretion positive regulation of brown fat cell differentiation positive regulation of protein localization to nucleus negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway negative regulation of feeding behavior
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